[1]熊剑,常慧君,李鹏,等.IBP促进人涎腺腺样囊性癌细胞对紫杉醇耐药[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):723-725.
 Xiong Jian,Chang Huijun,Li Peng,et al.IRF-4 binding protein promotes resistance of human salivary adenoid cystic carcinoma cells to taxol[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):723-725.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第08期
页码:
723-725
栏目:
论著
出版日期:
2012-04-30

文章信息/Info

Title:
IRF-4 binding protein promotes resistance of human salivary adenoid cystic carcinoma cells to taxol
作者:
熊剑常慧君李鹏范舒申涛简从相周继祥胡川闽
第三军医大学:西南医院口腔科,医学检验系临床生物化学教研室;咸宁学院口腔系;四川省军区门诊部口腔科
Author(s):
Xiong Jian Chang Huijun Li Peng Fan Shu Shen Tao Jian Congxiang Zhou Jixiang Hu Chuanmin
Department of Stomatology, Southwest Hospital,Department of Clinical Biochemistry, Faculty of Laboratory Medicine, Third Military Medical University, Chongqing, 400038; Department of Stomatology, Xianning University, Xianning, Hubei Province, 437100; Clinic of Stomatology, Department of Outpatients, Sichuan Military Command, Chengdu, Sichuan Province, 610041, China
关键词:
IBP紫杉醇免疫荧光染色Tubulin
Keywords:
IRF-4 binding protein taxol immune immunofluorescence staining tubulin
分类号:
R341;R739.87;R966
文献标志码:
A
摘要:
目的      探讨IBP对SACC细胞抗紫杉醇凋亡能力的影响及其机制。      方法      将ACC2转染IBP组和空白对照组各自根据不同紫杉醇浓度分成5组,紫杉醇作用72 h后,MTT法检测在紫杉醇作用下IBP对SACC细胞增殖的影响;通过微管蛋白Tubulin免疫荧光染色,观察紫杉醇作用前后ACC2细胞微管的变化及IBP与微管的关系。      结果      IBP使ACC2细胞对紫杉醇产生一定程度的耐药,在5 μg/ml的紫杉醇浓度下最为明显;紫杉醇开始作用后,ACC2-C1细胞的微管点状聚集成团,而ACC2-C1/IBP细胞的微管则出现明显的紊乱、断裂,IBP能促进微管的解聚;IBP所发的绿色荧光与微管的红色荧光糅合在一起呈黄色,IBP与微管存在一定程度的共定位。      结论      IBP促进SACC细胞对紫杉醇耐药。
Abstract:
Objective      To study the effect of IRF-4 binding protein (IBP) on resistance of salivary adenoid cystic carcinoma (SACC) cells to taxol and its mechanism.       Methods      ACC2 cells transfected with IBP and blank control cells (ACC2-C1 cells) were divided into 5 groups according to their taxol concentration. Effect of IBP on proliferation of SACC cells was detected by MTT assay 72 h after taxol was used. Occurrence of changes in microtubules of SACC cells and its relation with IBP were observed with tubulin immunofluorescence staining before and after taxol was used.       Results      IBP increased the resistance of ACC2 cells to taxol, especially at the concentration of 5 μg/ml. The point-like microtubules of ACC2-C1 cells aggregated into a clump while the microtubules of ACC2-C1/ IBP cells were arranged in disorder and fractured, indicating that IBP can promote depolymerization of microtubules. Green fluorescence of IBP and red fluorescence of microtubules were merged as a yellow color, showing that IBP and microtubules are located at the same site.       Conclusion      IBP promotes resistance of SACC cells to taxol.

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更新日期/Last Update: 2012-04-17