[1]周云涛,刘静,王志国,等.类风湿关节炎外周血CD4+CD161+T细胞亚群频数情况[J].陆军军医大学学报(原第三军医大学学报),2012,34(05):438-441.
 Zhou Yuntao,Liu Jing,Wang Zhiguo,et al.Frequency of peripheral blood CD161+CD4+ T cells in rheumatoid arthritis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(05):438-441.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第05期
页码:
438-441
栏目:
论著
出版日期:
2012-03-15

文章信息/Info

Title:
Frequency of peripheral blood CD161+CD4+ T cells in rheumatoid arthritis
作者:
周云涛刘静王志国郝丽宁刘畅周玉秀佟胜全田卫
唐山市工人医院:中心实验室,风湿病科
Author(s):
Zhou Yuntao Liu Jing Wang Zhiguo Hao Lining Liu Chang Zhou Yuxiu Tong Shengquan Tian Wei
Laboratory Center,Department of Rheumatic Diseases, Tangshan Gongren Hospital, Tangshan, Hebei Province, 063000, China
关键词:
类风湿关节炎CD161CD4+ T 细胞外周血单个核细胞
Keywords:
rheumatoid arthritis CD161 CD4+ T cell peripheral blood mononuclear cells
分类号:
R593.22; R446.113; R392.1
文献标志码:
A
摘要:
目的      检测外周血单个核细胞(peripheral blood mononuclear cells, PBMCs)中CD161+ T细胞的频数变化,探讨其在类风湿关节炎(RA)发生、发展中的作用。      方法        收集唐山市工人医院风湿病科2009年6月至2010年6月21例类风湿关节炎患者,其中男性7例,女性14例,平均年龄55岁。采用PBMCs体外活化技术,应用流式细胞术检测RA患者外周血PBMCs中CD4+CD161+ T细胞的频数情况。      结果       与年龄、性别匹配的健康人群相,RA患者PBMCs中CD161+CD4+ T和CD161+ Th1细胞的百分比显著降低[(27.53±7.34)% vs(22.75±5.11)%,(P<0.05);(11.35±4.36)% vs(8.21±3.93)%,(P<0.05)],导致RA患者CD161+与CD161- Th1细胞的比率下降,二者差异有显著性[(0.76±0.29)% vs(0.60±0.30)%,(P<0.05)]。      结论        RA患者滑膜炎性反应导致外周血CD161+CD4+T细胞及其Th1亚群与相应阴性T细胞群体的平衡紊乱,CD161+CD4+ T细胞及其Th1亚群在RA的病理过程密切相关。
Abstract:
Objective       To investigate the frequency of CD161+ T cells in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis (RA).       Methods       A total of 21 patients diagnosed as RA (14 women and 7 men, at an average age of 55) who hospitalized in our department from June 2009 to June 2010 were enrolled in this study. Another 21 age- and sex-matched healthy volunteers served as normal control. PBMCs were obtained from RA patients and healthy subjects. CD161 positive T cells were identified by a four-color flow cytometer after ex vivo stimulation with phorbol myristate acetate and ionomycin in the present of brefeldin A (BFA).       Results       The frequencies of CD161+ T cells and CD161+ Th1 cells were significantly decreased within CD4+ T cells of PBMCs from RA patients, respectively [(27.53±7.34)% vs (22.75±5.11)%, P<0.05; (11.35±4.36)% vs (8.21±3.93)%, P<0.05], which led to a decreased percentage of CD161+ and CD161-Th1 cells in RA patients.       Conclusion       The inflammatory response of synovial membrane in RA patients results in the changes of CD161+CD4+ T cells and CD161+ Th1 cells, rather than those of the corresponding CD161-T cells, which suggests CD161+ T cells might play an important role in the development of RA.

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更新日期/Last Update: 2012-03-02