[1]童欣,刘地川,吴泓权,等.超声微泡介导H-FABP基因改善慢性心衰大鼠的心功能[J].第三军医大学学报,2012,34(05):396-400.
 Tong Xin,Liu Dichuan,Wu Hongquan,et al.Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats[J].J Third Mil Med Univ,2012,34(05):396-400.
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超声微泡介导H-FABP基因改善慢性心衰大鼠的心功能(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第05期
页码:
396-400
栏目:
论著
出版日期:
2012-03-15

文章信息/Info

Title:
Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats
作者:
童欣刘地川吴泓权蔡敏
重庆医科大学附属第二医院心内科
Author(s):
Tong Xin Liu Dichuan Wu Hongquan Cai Min
Department of Cardiology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, China
关键词:
超声微泡脂肪酸结合蛋白心力衰竭游离脂肪酸
Keywords:
ultrasound microbubble fatty acid-binding protein heart failure free fatty acid
分类号:
R541.610.5;R445.1;R459.9
文献标志码:
A
摘要:
目的      研究超声靶向破坏微泡(ultrasound targeted microbubble destruction,UTMD)技术转染H-FABP基因对心肌梗死后心功能的影响。      方法        通过结扎成年雄性Wistar大鼠46只,体质量(220±20)g,冠状动脉前降支建立心肌梗死模型。于心肌梗死8周后,将存活的31只心衰大鼠随机分为4组:①H-FABP+超声+微泡组(H-FABP+US/MB组,n=8),用超声破坏携H-FABP基因微泡转染大鼠心肌;②超声+微泡组(US/MB组,n=8),用超声破坏不含基因的微泡;③H-FABP+生理盐水组(H-FABP+NS组,n=8),由颈静脉输入含基因的生理盐水;④单纯手术组(SA组,n=7), 仅由颈静脉输入生理盐水;⑤假手术组(SS组,n=6)。基因转染14 d后测定各组大鼠心功能,Western blot检测左心室非梗死区H-FABP表达,ELISA法检测静脉血及非梗死区心肌游离脂肪酸(FFA)含量,硫代巴比妥酸反应物(TBARS)法检测非梗死区心肌组织脂质过氧化水平,免疫组织化学法检测非梗死区心肌诱导型一氧化氮合酶(iNOS)表达。      结果       心肌梗死大鼠各组与假手术组比较心功能明显下降(P<0.05),H-FABP表达明显下降(P<0.05),静脉血及心肌FFA含量明显升高(P<0.05),丙二醛(MDA)水平明显升高(P<0.05),iNOS表达明显升高(P<0.05)。H-FABP+超声+微泡组与心肌梗死大鼠中的另外3组比较心功能明显改善(P<0.05),H-FABP表达升高(P<0.05),心肌FFA含量降低(P<0.05),MDA水平降低(P<0.05),iNOS表达受抑制(P<0.05)。      结论        超声微泡介导的H-FABP转染可改善大鼠慢性心衰时心功能。可能与通过提高H-FABP表达、降低心肌FFA含量、改善心肌氧化应激水平、进而抑制iNOS的表达有关。
Abstract:
Objective        To determine the effect of heart fatty acid binding protein (H-FABP) transfected by ultrasound targeted microbubble destruction (UTMD) technology on cardiac function after post-infarction.       Methods        Myocardial infarction (MI) was induced in Wistar male rats (n=46, 220±20 g) by ligating the left anterior descending coronary artery. After 8 weeks, the 31 surviving rats with heart failure were randomly divided into 4 groups, H-FABP+ultrasound+microbubble group (H-FABP+US/MB group, n=8, ultrasound-targeted destruction microbubble loaded with H-FABP gene was transfected to rat heart), ultrasound+microbubble group (US/MB group, n=8, ultrasonic destruction of microbubbles loaded without gene), H-FABP+normal saline group (H-FABP+NS group, n=8, normal saline containing gene were infused from jugular vein), surgery alone group (SA group, n=7, only normal saline were infused from jugular vein), and sham-operation group (SS group, n=6). In 14 d after transfection, the cardiac function of all the rats were measured, H-FABP expression in non-infarct region of left ventricular was detected by Western blotting, free fatty acid (FFA) content of venous blood and non-infarct region myocardium were detected by ELISA, lipid peroxidation in non-infarct region of left ventricular was detected by thiobarbituric acid reactive substances (TBARS) method, and the iNOS expression in non-infarct region of left ventricular was detected by immunohistochemical assay.       Results       Compared with sham-operation group, left ventricular ejection fraction (LVEF) and the H-FABP expression were decreased significantly (P<0.05), free fatty acid content and malondialdehyde (MDA) level and the iNOS expression were obviously increased (P<0.05) in the MI groups. Compared with US/MB, H-FABP+NS and SA groups, LVEF and the H-FABP expression were apparently increased (P<0.05), free fatty acid content of myocardium and MDA level were obviously decreased (P<0.05) and the iNOS expression was markedly inhibited (P<0.05) in H-FABP+US/MB group.       Conclusion       Ultrasound microbubble-mediated H-FABP transfection improves cardiac function via upregulating H-FABP, redu-cing myocardial FFA, ameliorating myocardial oxidative stress and inhibiting iNOS expression.

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更新日期/Last Update: 2012-03-01