[1]李颖,陈建斌,宋君君,等.甲基化修饰致PCDH10基因在多发性骨髓瘤中沉默[J].第三军医大学学报,2012,34(09):817-820.
 Li Ying,Chen Jianbin,Song Junjun,et al.Methylation induces protocadherin-10 silence in multiple myeloma[J].J Third Mil Med Univ,2012,34(09):817-820.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第09期
页码:
817-820
栏目:
论著
出版日期:
2012-05-15

文章信息/Info

Title:
Methylation induces protocadherin-10 silence in multiple myeloma
作者:
李颖陈建斌宋君君杨泽松向廷秀黄宗干
重庆医科大学附属第一医院:血液科,血液科实验室,分子肿瘤及表观遗传学实验室
Author(s):
Li Ying Chen Jianbin Song Junjun Yang Zesong Xiang Tingxiu Huang Zonggan
Department of Hematology, Laboratory of Hematology, Laboratory of Molecular Oncology and Epigenetics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
关键词:
多发性骨髓瘤PCDH10DNA甲基化
Keywords:
multiple myelomaprotocadherin-10DNA methylation
分类号:
R394-33; R73-362; R738.1
文献标志码:
A
摘要:
目的       研究PCDH10基因在多发性骨髓瘤(multiple myeloma,MM)患者及细胞系KM3、RPMI-8226中的甲基化状态,分析甲基化对其沉默的影响。      方法        常规骨髓穿刺术获取MM患者骨髓样本,培养骨髓瘤细胞系KM3、RPMI-8226,以增生性骨髓象骨髓样本作为对照。RT-PCR检测其中PCDH10基因表达水平,甲基化特异性PCR(methylation-sensitive PCR,MSP)法检测PCDH10基因甲基化状态,通过5-氮杂-2′-脱氧胞苷和组蛋白去乙酰化酶抑制剂[5-aza-2′-deoxycytidine (Aza) and trichostatin A (TSA),A+T]处理MM细胞系,检测药物处理前后PCDH10甲基化状态及表达水平。      结果        PCDH10基因在正常人群中表达,但在MM患者骨髓及细胞系中广泛低表达,且77.27%(34/44)的MM患者PCDH10呈甲基化状态。PCDH10基因可经A+T试验逆转甲基化状态并恢复表达。      结论        DNA甲基化可能在多发性骨髓瘤的PCDH10基因沉默中发挥作用,从而参与骨髓瘤的发生。
Abstract:
Objective        To determine the methylation status of protocadherin-10 (PCDH10) in multiple myeloma (MM) patients and MM cell lines KM3 and RPMI-8226, and investigate the effect of its methylation on PCDH10 silence.       Methods        A total of 44 MM tissue samples from January to December 2010 hospitalized in our department were collected. Bone marrow tissues of 9 age- and sex-matched patients with mild anemia obtained by conventional bone marrow biopsy served as control. The expression of PCDH10 gene was detected by RT-PCR in above tissue samples, and multiple myeloma cell lines KM3 and RPMI-8226. Methylation-sensitive PCR (MSP) was used to test the methylation of PCDH10 CpG islands. Then, after the KM3 and RPMI-8226 cells were treated with 5-aza-2′-deoxycytidine and trichostatin A (Aza and TSA, A+T), the changes of PCDH10 were studied by RT-PCR and MSP.       Results        PCDH10 was expressed well in normal population while extensive mildly expressed in MM patients and cell lines. Among the 44 patients (23 females, 21 males), 77.27% (34/44) whom presented methylation of PCDH10. Furthermore, A+T restored PCDH10 expression in myeloma cell lines.       Conclusion        DNA methylation may play a key role in the silence of PCDH10 gene in multiple myeloma, and may be involved in the incidence of myeloma.

参考文献/References:

李颖, 陈建斌, 宋君君, 等. 甲基化修饰致PCDH10基因在多发性骨髓瘤中沉默[J].第三军医大学学报,2012,34(9):817-820.

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更新日期/Last Update: 2012-05-07