[1]朱剑武,夏蕾,杨剑,等.白藜芦醇抑制A549人肺腺癌细胞增殖与IGF-ⅠR的关系[J].第三军医大学学报,2011,33(24):2567-2570.
 Zhu Jianwu,Xia Lei,Yang Jian,et al.Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR[J].J Third Mil Med Univ,2011,33(24):2567-2570.
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白藜芦醇抑制A549人肺腺癌细胞增殖与IGF-ⅠR的关系(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第24期
页码:
2567-2570
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR
作者:
朱剑武夏蕾杨剑罗维李娟周芊曾林立王东杨镇洲
第三军医大学大坪医院野战外科研究所:肿瘤中心,营养科
Author(s):
Zhu Jianwu Xia Lei Yang Jian Luo Wei Li Juan Zhou Qian Zeng Linli Wang Dong Yang Zhenzhou
Center of Oncology, Department of Nutrition, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042,  China
关键词:
白藜芦醇胰岛素样生长因子-1胰岛素样生长因子-1受体人肺腺癌细胞A549
Keywords:
resveratrol IGF-Ⅰ IGF-ⅠR A549 human lung adenocarcinoma cells
分类号:
R730.23;R734.2;R979.19
文献标志码:
A
摘要:
目的      探讨白藜芦醇对人肺腺癌A549细胞增殖的抑制效应及其与IGF-ⅠR的关系。      方法      以人肺腺癌A549细胞株为研究对象,采用MTT方法测定细胞增殖,用RT-PCR与免疫印迹方法测定IGF-Ⅰ受体mRNA与蛋白表达水平。      结果      2.5~15 nmol/L IGF-Ⅰ可显著促进A549细胞增殖(P<0.05),给予12.5、25.0、50 μmol/L白藜芦醇对A549细胞增殖具有明显抑制作用(P<0.05)。白藜芦醇(6.25~50 μmol/L)与10 nmol/L IGF-Ⅰ共作用于A549细胞,均可抑制IGF-Ⅰ的促A549细胞增殖作用,其中尤以25、50 μmol/L白藜芦醇的抑制增殖作用显著(P<0.05),白藜芦醇可有效降低IGF-ⅠR mRNA与蛋白表达。      结论      白藜芦醇可通过降低IGF-ⅠR mRNA与蛋白表达有效抑制IGF-Ⅰ介导的A549肺癌细胞增殖。
Abstract:
Objective      To study the correlation between inhibitory effect of resveratrol on proliferation of A549 human lung adenocarcinoma cells and IGF-ⅠR.       Methods      Proliferation of A549 human lung andenocarcinoma cells was detected by methyl thiazolyl tetrazolium (MTT) assay. Expression level of IGF-ⅠR mRNA and protein was measured by RT-PCR and Western blotting, respectively.       Results      IGF-Ⅰ at the concentration of 2.5 to 15 nmol/L significantly increased the proliferation of A549 human lung andenocarcinoma cells (P<0.05) and resveratrol at the concentrations of 12.5, 25.0  and 50 μmol/L remarkably inhibited the proliferation of A549 human lung andenocarcinoma cells (P<0.05). Combined resveratrol (6.25 to 50 μmol/L) and IGF-Ⅰ (10 nmol/L) could inhibit the IGF-ⅠR-induced proliferation of A549 human lung andenocarcinoma cells, especially at the concentrations of 25.0  and 50.0 μmol/L (P<0.05). Resveratrol could effectively down-regulate the expression of IGF-ⅠR mRNA and protein.       Conclusion      Resveratrol can effectively inhibit the IGF-ⅠR-induced proliferation of A549 human lung adenocarcinoma cells by down-regulating the expression of IGF-ⅠR mRNA and protein.

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更新日期/Last Update: 2011-12-15