[1]陈雪梅,张学东,宗元娟,等.高糖对视网膜色素上皮细胞Toll样受体2表达的影响[J].第三军医大学学报,2012,34(01):70-73.
 Chen Xuemei,Zhang Xuedong,Zong Yuanjuan,et al.Effect of high glucose concentration on expression of toll-like receptor-2 in ARPE-19 cells[J].J Third Mil Med Univ,2012,34(01):70-73.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第01期
页码:
70-73
栏目:
论著
出版日期:
2012-01-15

文章信息/Info

Title:
Effect of high glucose concentration on expression of toll-like receptor-2 in ARPE-19 cells
作者:
陈雪梅张学东宗元娟沈强
重庆医科大学附属第一医院眼科,眼科学重庆市市级重点实验室
Author(s):
Chen Xuemei Zhang Xuedong Zong Yuanjuan Shen Qiang
Department of Ophthalmology, Chongqing Key Laboratory of Ophthalmology, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
糖尿病视网膜病变ARPE-19Toll样受体2高糖
Keywords:
diabetic retinopathyARPE-19toll-like receptor-2high glucose concentration
分类号:
R587.202; R774.102; R362
文献标志码:
A
摘要:
目的      观察高糖对体外培养的ARPE-19细胞Toll样受体2(toll-like receptor 2,TLR2)表达的影响及机制。      方法        各组ARPE-19(RPE细胞系)细胞分别刺激48 h,正常糖浓度组(5.5 mmol/L D-葡萄糖)、中度高糖组(15.5 mmol/L D-葡萄糖)、高糖组(25.5 mmol/L D-葡萄糖)、PKC-α抑制剂组(25.5 mmol/L D-葡萄糖加10 mmol/L Go6976 PKC-α抑制剂)、高渗对照组(5.5 mmol/L D-葡萄糖加20 mmol/L甘露醇)。Western blot检测各组ARPE-19细胞TLR2蛋白表达水平,以及PKC-α蛋白胞浆向胞膜的转位情况;Real-time PCR分析各组ARPE-19细胞TLR2的mRNA表达水平。      结果        与正常糖浓度组相比,高糖组和中度高糖组TLR2的mRNA和蛋白表达,以及PKC-α转位均增加(P<0.05),并且高糖组高于中度高糖组(P<0.05)。与高糖组比较,PKC-α抑制剂组的TLR2蛋白表达降低(P<0.05),PKC-α蛋白转位显著降低(P<0.01)。      结论        高糖可以上调ARPE-19细胞TLR2的mRNA和蛋白表达,刺激PKC-α转位;高糖可能是通过PKC-α通路上调ARPE-19细胞TLR2表达。
Abstract:
Objective       To observe the effect of different glucose concentrations on expression of toll-like receptor-2 (TLR-2) in ARPE-19 cells.       Methods        ARPE-19 cells were divided into 5.5 mmol/L glucose group, 15.5 mmol/L glucose group, 25.5 mmol/L glucose, 25.5 mmol/L glucose +10 mmol/L Go6976 PKC-α inhibition group, 5.5 mmol/L glucose + 20 mmol/L mannitol control group. Expression level of TLR-2 in ARPE-19 cells and translocation of PKC-α from cytoplasm to cytomembrane were measured by Western blotting, while that of TLR-2 mRNA in ARPE-19 cells was measured by real-time PCR.       Results        The expression level of TLR-2 mRNA and the translocation of PKC-α from cytoplasm to cytomembrane were higher in 25.5 mmol/L glucose group and 15.5 mmol/L glucose group than in control group (P<0.05). The expression level of TLR-2 protein and the translocation of PKC-α from cytoplasm to cytomembrane were significantly lower in PKC-α inhibitor inhalation group than in 25.5 mmol/L glucose group (P<0.01).       Conclusion        High glucose concentration can up-regulate the expression of TLR2 mRNA in ARPE-19 cells and promote the translocation of PKC-α from cytoplasm to cytomembrane through the PKC-α pathway.

参考文献/References:

陈雪梅, 张学东, 宗元娟, 等. 高糖对视网膜色素上皮细胞Toll样受体2表达的影响[J].第三军医大学学报,2012,34(1):70-73.

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更新日期/Last Update: 2012-01-04