[1]邹正渝,陈英华,孙双双,等.hS100A6抑制卵巢癌细胞HO8910和HO8910 PM增殖、促进凋亡并上调其Wnt/β-catenin 活性[J].陆军军医大学学报(原第三军医大学学报),2012,34(03):214-218.
 Zou Zhengyu,Chen Yinghua,Sun Shuangshuang,et al.hS100A6 inhibits proliferation, induces apoptosis and up-regulates Wnt/β-catenin activity in ovarian cancer cell lines HO8910 and HO8910 PM[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(03):214-218.
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hS100A6抑制卵巢癌细胞HO8910和HO8910 PM增殖、促进凋亡并上调其Wnt/β-catenin 活性(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第03期
页码:
214-218
栏目:
论著
出版日期:
2012-02-15

文章信息/Info

Title:
hS100A6 inhibits proliferation, induces apoptosis and up-regulates Wnt/β-catenin activity in ovarian cancer cell lines HO8910 and HO8910 PM
作者:
邹正渝陈英华孙双双黎玉叶叶立伟段亮武睿杨霞罗进勇周兰
重庆医科大学医学检验系临床检验诊断学教育部重点实验室;重庆市第四人民医院肿瘤科;重庆医科大学附属口腔医院口腔黏膜科
Author(s):
Zou Zhengyu Chen Yinghua Sun Shuangshuang Li Yuye Ye Liwei Duan Liang Wu Rui Yang Xia Luo Jinyong Zhou Lan
Key Laboratory of Laboratory Medical Diagnostics Cofounded by Chongqing and Ministry of Education, Faculty of Medical Laboratory, Chongqing Medical University, Chongqing, 400016; Department of Oncology, Fourth People’s Hospital of Chongqing, Chongqing, 400015; Department of Periodontal Mucosa, Affiliated Hospital of Stomatological, Chongqing Medical University, Chongqing, 400015, China
关键词:
S100A6卵巢癌Wnt/β-catenin信号途径
Keywords:
S100A6 ovarian cancer Wnt/β-catenin signaling pathway
分类号:
R341; R730.23; R737.31
文献标志码:
A
摘要:
目的      研究hS100A6(human S100A6)对人卵巢癌细胞系(低转移系HO8910和高转移系HO8910 PM)增殖、凋亡和Wnt/β-catenin信号途径的影响。      方法        通过MTT以及台盼蓝染色检测细胞增殖的变化;Hoechst检测细胞凋亡的变化;提取细胞总蛋白及核蛋白Western blot检测hS100A6对β-catenin的影响。       结果        MTT及台盼蓝染色结果显示hS100A6抑制HO8910细胞和HO8910 PM细胞的增殖(P<0.05),Hoechst染色结果显示hS100A6同时促进这两系细胞的凋亡(P<0.05);Western blot显示hS100A6增加β-catenin的水平并促进其发生核转位。       结论        hS100A6能抑制人卵巢癌细胞系HO8910和HO8910 PM增殖,促进其凋亡,同时激活Wnt/β-catenin信号途径。
Abstract:
Objective        To investigate the effect of human S100A6 (hS100A6) on the proliferation, apoptosis and Wnt/β-catenin signaling pathway of ovarian cancer cell line HO8910 and HO8910 PM.       Methods       MTT assay and trypan blue exclusion assay, Hoechst staining and Western blotting were used to detect cell proliferation, apoptosis and expression and distribution of β-catenin, respectively.       Results        ① hS100A6 inhibited proliferation of HO8910 and HO8910 PM cells in a dose- and time-dependent manner (P<0.05); ② hS100A6 induced apoptosis in HO8910 and HO8910 PM cells (P<0.05); ③ Level of β-catenin was increased by hS100A6 in HO8910 and HO8910 PM cells, and β-catenin translocated from cytoplasm to nucleus.       Conclusion        hS100A6 can inhibit cell proliferation, induce cell apoptosis and up-regulate Wnt/β-catenin activity in ovarian cancer HO8910 and HO8910 PM cells.

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更新日期/Last Update: 2012-02-08