[1]贾朝莉,龙方懿,刘智敏,等.氯化钴对甲状腺乳头状癌NPA细胞上皮间质转化的影响及其机制[J].第三军医大学学报,2012,34(02):150-153.
 Jia Chaoli,Long Fangyi,Liu Zhimin,et al.CoCl2 improves epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J].J Third Mil Med Univ,2012,34(02):150-153.
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氯化钴对甲状腺乳头状癌NPA细胞上皮间质转化的影响及其机制(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第02期
页码:
150-153
栏目:
论著
出版日期:
2012-01-30

文章信息/Info

Title:
CoCl2 improves epithelial-mesenchymal transition of human papillary thyroid carcinoma cells
作者:
贾朝莉龙方懿刘智敏杨俊艳刘小花董超然王妮
重庆医科大学基础医学院生物化学与分子生物学教研室,分子医学与肿瘤研究中心
Author(s):
Jia Chaoli Long Fangyi Liu Zhimin Yang Junyan Liu Xiaohua Dong ChaoranWang Ni
Department of Biochemistry and Molecular Biology, Institute of Molecular Medicine and Cancer, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
低氧诱导因子-1α上皮间质转化甲状腺乳头状癌
Keywords:
hypoxia-inducible factor-1α epithelial-mesenchymal transition papillary thyroid carcinoma
分类号:
R363.25;R730.23;R736.1
文献标志码:
A
摘要:
目的      研究氯化钴(CoCl2)诱导的低氧对甲状腺乳头状癌NPA细胞上皮间质转化的影响,初步探讨HIF-1α在其中的机制。      方法      MTT检测CoCl2(50、100、150、200、250 μmol/L)对细胞活力的影响,倒置显微镜观察低氧(150 μmol/L的CoCl2分别作用0、12、24、48 h)对细胞形态的影响,Western blot检测低氧(150 μmol/L的CoCl2分别作用0、3、6、12、24 h)对HIF-1α、E-cadherin和Vimentin表达的影响,Transwell法检测低氧(150 μmol/L的CoCl2分别作用0、6 h)对细胞侵袭、转移的影响,用免疫荧光对HIF-1α蛋白进行亚细胞定位。      结果      低氧模拟剂CoCl2可抑制NPA细胞活力。随着CoCl2低氧处理时间的延长,NPA细胞逐渐获得间质细胞的形态特征,HIF-1α、Vimentin表达在0~6 h逐渐增加,随后开始下降,E-cadherin表达持续降低,低氧0 h与6 h相比各蛋白表达均具有统计学差异(P<0.01)。低氧组侵袭、转移细胞数目较对照组明显增加(P<0.01),同时低氧组细胞HIF-1α发生核转位。      结论      CoCl2模拟化学性低氧可促进NPA细胞上皮间质转化及侵袭、转移,其分子机制可能涉及HIF-1α介导的信号通路对E-cadherin、Vimentin基因表达的调控。
Abstract:
Objective      To determine the effect of CoCl2 induced hypoxia on the epithelial-mesenchymal transition in human papillary thyroid carcinoma and investigate the role of HIF-1α in this process.       Methods      Cell vitality was evaluated by MTT assay in human papillary thyroid carcinoma NPA cells after the treatment of 50, 100, 150, 200 and 250 μmol/L CoCl2. Morphological changes of NPA cells were observed by inverted microscopy after 150 μmol/L CoCl2 treatment for 0, 12, 24 and 48 h. The expression levels of HIF-1α, E-cadherin and Vimentin were analyzed by Western blotting in NPA cells after 150 μmol/L CoCl2 treatment for 0, 3, 6, 12, and 24 h. The invasion and metastasis potential in NPA cells after 150 μmol/L CoCl2 treatment for 0 and 6 h was detected by Transwell assay. Immunofluorescence was used to detect the location of HIF-1α in NPA cells.       Results      CoCl2 treatment resulted in suppression of NPA cell vitality in a dose-dependent manner. The longer CoCl2 was treated, NPA cells gradually acquired the morphological characteristics of mesenchymal cells. The expression of HIF-1α, E-cadherin and Vimentin was gradually enhanced in 0 to 6 h after treatment, and then declined, especially in E-cadherin. Significant differences were found in the expression levels of these proteins at 0 h and 6 h (P<0.01). There were more cells that penetrated through the Transwell membrane in hypoxia group than in normoxic group (P<0.01). HIF-1α translocated to the nucleus in hypoxia group, while it didn’t occur in normoxia group.       Conclusion      CoCl2 treatment, which mimics chemical hypoxia improves the epithelial-mesenchymal transition, invasion and migration of NPA cells, which might be due to that HIF-1α mediated signal pathway regulates E-cadherin and Vimentin.

参考文献/References:

贾朝莉, 龙方懿, 刘智敏, 等. 氯化钴对甲状腺乳头状癌NPA细胞上皮间质转化的影响及其机制[J].第三军医大学学报,2012,34(2):150-153.

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更新日期/Last Update: 2012-01-13