[1]刘安全,吴小候,罗春丽.HepaCAM蛋白通过exosomes途径分泌到细胞外并抑制肿瘤细胞增殖[J].第三军医大学学报,2012,34(02):168-171.
 Liu Anquan,Wu Xiaohou,Luo Chunli.HepaCAM secreted by renal cell carcinoma 786-0 cells through exosomes pathway inhibits cell proliferation[J].J Third Mil Med Univ,2012,34(02):168-171.
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HepaCAM蛋白通过exosomes途径分泌到细胞外并抑制肿瘤细胞增殖(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第02期
页码:
168-171
栏目:
论著
出版日期:
2012-01-30

文章信息/Info

Title:
HepaCAM secreted by renal cell carcinoma 786-0 cells through exosomes pathway inhibits cell proliferation
作者:
刘安全吴小候罗春丽
重庆医科大学:附属第一医院泌尿外科,检验医学院
Author(s):
Liu Anquan Wu Xiaohou Luo Chunli
Department of Urology, First Affiliated Hospital, Faculty of Clinical Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China
关键词:
肝细胞粘附分子exosomes分泌增殖
Keywords:
hepatocyte cell adhesion molecule exosomes secretion proliferation
分类号:
R73-36;R737.11;R394.2
文献标志码:
A
摘要:
目的      探讨肝细胞黏附分子(hepatocyte cell adhesion molecule, hepaCAM)在肾癌786-0细胞中通过exosomes途径分泌到细胞外及是否可以抑制肿瘤细胞增殖。      方法      将携带hepaCAM基因的重组腺病毒质粒瞬时转染786-0细胞,Western blot鉴定hepaCAM基因在786-0中的表达;莫能星(monensin)和二甲基阿米洛利(DMA)处理转染后肿瘤细胞,检测肿瘤细胞上清液及exosomes中hepaCAM蛋白的量的变化。MTT法检测hepaCAM蛋白是否可以抑制肿瘤细胞增殖。      结果      成功构建并转染hepaCAM重组腺病毒质粒,在肿瘤细胞上清液中检测到hepaCAM 蛋白的分泌,经药物处理后,莫能星明显增加了转染后肿瘤细胞的hepaCAM蛋白的分泌量,而二甲基阿米洛利处理转染后肿瘤细胞,能显著抑制细胞上清液中hepaCAM的分泌量。Western blot进一步证明hepaCAM 蛋白存在于exosomes上。成功转染hepaCAM基因的肾癌细胞分泌的exosomes明显抑制了肿瘤细胞增殖。      结论      hepaCAM蛋白可能通过exosomes途径分泌到肿瘤细胞外并抑制肿瘤细胞增殖。
Abstract:
Objective      To investigate that whether hepatocyte cell adhesion molecule (hepaCAM) is actively secreted from renal cell carcinoma 786-0 cells by a pathway involving exosomes and whether hepaCAM could inhibit proliferation in 786-0 cells.       Methods      A recombinant adenovirus vector plasmid containing hepaCAM was transfected into 786-0 cells. The expression of hepaCAM was detected by Western blotting. The transfected cells were treated with monensin and 5-(N-N-Dimetyl)-amiloride hydrochloride (DMA), and then extracellular culture medium and exosomes were used to investigate the content of hepaCAM by Bradford protein assay. MTT assay was used to detect the cell viability after treatment of exosomes cotaining hepaCAM.       Results      HepaCAM adenovirus vector plasmid was successfully constructed and stably transfected into 786-0 cells. Monensin enhanced the release of hepaCAM while DMA inhibited the process in the transfected 786-0 cells. Exosomes purityed from the supernatant of transfected 786-0 cells inhibited the proliferation of 786-0 cells obvously.       Conclusion      HepaCAM is secreted from renal cell carcinoma cells by a pathway involving exosomes, and inhibits cell proliferation at the same time.

参考文献/References:

刘安全, 吴小候, 罗春丽. HepaCAM蛋白通过exosomes途径分泌到细胞外并抑制肿瘤细胞增殖[J].第三军医大学学报,2012,34(2):168-171.

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更新日期/Last Update: 2012-01-13