[1]胡长江,张艳,黄刚,等.TO901317抑制叉头盒蛋白M1表达并影响HepG2细胞增殖[J].陆军军医大学学报(原第三军医大学学报),2011,33(22):2345-2348.
 Hu Changjiang,Zhang Yan,Huang Gang,et al.TO901317 suppresses FOXM1 expression and proliferation in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(22):2345-2348.
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TO901317抑制叉头盒蛋白M1表达并影响HepG2细胞增殖(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第22期
页码:
2345-2348
栏目:
论著
出版日期:
2011-11-30

文章信息/Info

Title:
TO901317 suppresses FOXM1 expression and proliferation in HepG2 cells
作者:
胡长江张艳黄刚张立申晓冬高敏何谐曾益军何凤田
第三军医大学基础医学部生物化学与分子生物学教研室
Author(s):
Hu Changjiang Zhang Yan Huang Gang Zhang Li Shen Xiaodong Gao Min He Xie Zeng Yijun He Fengtian
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
关键词:
TO901317叉头盒蛋白M1HepG2细胞
Keywords:
TO901317 forkhead box protein M1 HepG2 cells
分类号:
R735.7;R394.3;R730.23
文献标志码:
A
摘要:
目的      探讨肝X受体(liver X receptors,LXR)特异性激动剂TO901317对HepG2细胞中叉头盒蛋白M1(forkhead box protein M1,FOXM1)的表达及对HepG2细胞的增殖及细胞周期的影响。      方法      TO901317(终浓度分别为0.5、5 μmol/L)处理HepG2细胞24 h后,采用RT-PCR和Western blot检测FOXM1  mRNA和蛋白表达情况;采用流式细胞术检测细胞周期的变化,并用CCK-8检测HepG2细胞增殖情况。      结果        经不同浓度的TO901317(终浓度0.5、5 μmol/L)处理HepG2细胞24 h后,FOXM1 mRNA和蛋白表达呈剂量依赖性下调(P<0.05);流式细胞仪检测结果显示,对照组G1期细胞数占间期所有细胞数的百分比为(52.98±2.53)%、0.5 μmol/L TO901317处理组为(61.32±2.36)%、5 μmol/L TO901317处理组为(70.40±4.65)%,表明TO901317能够剂量依赖性诱导HepG2细胞G1期阻滞(P<0.05);CCK-8检测结果显示,对照组D(450)为(1.53±0.07)、0.5 μmol/L TO901317处理组为(1.25±0.09)、5 μmol/L TO901317处理组为(1.06±0.06),表明TO901317能够剂量依赖性抑制HepG2细胞增殖(P<0.05)。      结论      肝X受体诱导HepG2细胞的G1期阻滞,并且抑制该细胞的增殖,其原因之一可能由于抑制了细胞周期蛋白关键调节因子FOXM1的表达。
Abstract:
Objective      To investigate the effect of treatment of live X receptors specific agonist TO901317 on the expression of FOXM1 and the cell cycle and proliferation in HepG2 cells.       Methods      HepG2 cells were treated for 24 h with DMSO and 0.5 or 5 μmol/L TO901317. RT-PCR and Western blotting was taken to detect the FOXM1 expression at mRNA and protein levels. Cell cycle analysis was examined by flow cytometry and cell proliferation was determined by cell counting kit-8 (CCK-8).       Results      FOXM1 mRNA and protein expression were remarkably reduced after the treatment of TO901317 at different concentrations (P<0.05). The percentage of cells at G1 stage were (61.32±2.36)% and (70.40±4.65)% respectively after 0.5 and 5 μmol/L TO901317 treatment, significantly higher than that of the control group (P<0.05). The OD value at 450 nm was  1.25±0.09 and 1.06±0.06 respectively after 0.5 and 5 μmol/L TO901317 treatment, significantly lower than the control group (1.53±0.07, P<0.05). TO901317 notablely inhibited the HepG2 cells proliferation.       Conclusion      TO901317 could suppress HepG2 proliferation and induce cell arrest at G1 cycle in a does-dependent manner, and it may be result from the downregulation of FOXM1,which is one of the key transcription regulators of cell cycle proteins.

相似文献/References:

[1]姜珊,熊浩君,何家琳,等.miR-134通过下调叉头盒蛋白M1抑制肝细胞癌细胞增殖[J].陆军军医大学学报(原第三军医大学学报),2017,39(01):1.
 Jiang Shan,Xiong Haojun,He Jialin,et al.miR-134 suppresses proliferation in hepatocellular carcinoma cells by down-regulating FOXM1[J].J Amry Med Univ (J Third Mil Med Univ),2017,39(22):1.

更新日期/Last Update: 2011-11-21