[1]卓燕,余荣杰,赵洪雯,等.阿托伐他汀抑制ApoE-/-小鼠腹腔巨噬细胞LPS刺激下NF-κB p65核转位[J].第三军医大学学报,2011,33(20):2120-2123.
 Zhuo Yan,Yu Rongjie,Zhou Hongwen,et al.Atorvastatin suppresses nuclear translocation of NF-κB p65 in LPS-induced abdominal macrophage of ApoE-/- mouse[J].J Third Mil Med Univ,2011,33(20):2120-2123.
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阿托伐他汀抑制ApoE-/-小鼠腹腔巨噬细胞LPS刺激下NF-κB p65核转位(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第20期
页码:
2120-2123
栏目:
论著
出版日期:
2011-10-30

文章信息/Info

Title:
Atorvastatin suppresses nuclear translocation of NF-κB p65 in LPS-induced abdominal macrophage of ApoE-/- mouse
作者:
卓燕余荣杰赵洪雯吴雄飞
第三军医大学西南医院肾科
Author(s):
Zhuo Yan Yu Rongjie Zhou Hongwen Wu Xiongfei
Department of Kidney, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
阿托伐他汀脂多糖巨噬细胞NF-κB p65小鼠基因敲除
Keywords:
atorvastatinLipopolysaccharidesabdominal macrophageNF-κB p65 mice knockout
分类号:
R692;R966;R972.6
文献标志码:
A
摘要:
目的      观察阿托伐他汀对脂多糖(lipopolysaccharides,LPS)刺激载脂蛋白E基因缺陷(apolipoprotein E knockout,ApoE-/-)小鼠腹腔巨噬细胞NF-κB p65核转位的影响。      方法      采用LPS 10 ng/ml刺激分离纯化的ApoE-/-小鼠腹腔巨噬细胞,并给予1、5、10 μmol/L不同浓度的阿托伐他汀进行干预,免疫荧光染色结合激光共聚焦显微镜观察NF-κB p65核转位,Western blot法检测NF-κB p65核内表达,ELASA法检测细胞上清液IL-6水平。      结果      激光共聚焦及Western blot均观察到阴性对照组核内NF-κB p65表达较低,其平均荧光强度为(3.71±0.26);阳性对照组核内NF-κB p65表达较阴性对照组显著增高[(16.12±1.28),P<0.05];不同剂量阿托伐他汀干预组细胞核内NF-κB p65表达呈剂量依耐性逐渐降低,其平均荧光强度分别为(10.95±0.71)、(7.61±0.73)、(4.56±0.39),差异具有统计学意义(P<0.05);同时可发现药物干预组细胞上清液IL-6分泌量也呈剂量依耐性逐渐降低(P<0.05)。      结论      阿托伐他汀能抑制ApoE-/-小鼠腹腔巨噬细胞LPS刺激下NF-κB  p65核转位及其随后炎性介质IL-6分泌。
Abstract:
Objective      To determine the effects of atorvastatin on the translocation of NF-κB p65 into the nuclear induced by LPS in abdominal macrophage isolated from apolipoprotein E knockout (ApoE-/-) mouse.       Methods      Abdominal macrophage were isolated and purified from ApoE-/- mouse, and then cultivated and treated by atorvastatin (10 μmol/L), LPS (10 ng/ml), and atorvastatin (1, 5 and 10 μmol/L) combined with LPS treatment. The nuclear translocation of NF-κB p65 was observed by laser scanning confocal microscopy and Western blotting, while the IL-6 levels in culture supernatants was measured by ELASA.       Results      Laser scanning confocal microscopy and Western blotting showed that TRITC-labeled NF-κB p65 was mainly located in the plasma of macrophage and mildly expressed in the nuclear in atorvastatin group (3.71±0.26), but was mainly located in the nuclei and strongly expressed in LPS group (16.12±1.28, P<0.05). When macrophage were cultivated and induced by LPS and different concentrations atorvastatin, NF-κB p65 was expressed in the nuclear in a negative dose-dependent manner (10.95±0.71, 7.61±0.73, and 4.56±0.39, respectively, P<0.05). At the same time, the IL-6 levels in culture supernatants was also reduced in a negative dose-dependent manner in atorvastatin intervention groups(P<0.05).       Conclusion      Atorvastatin inhibits the nuclear translocation of NF-κB p65 and secretion of inflammatory mediators IL-6 in abdominal macrophage of ApoE-/- mouse induced by LPS.

参考文献/References:

卓燕, 余荣杰, 赵洪雯, 等. 阿托伐他汀抑制ApoE-/-小鼠腹腔巨噬细胞LPS刺激下NF-κB p65核转位[J].第三军医大学学报,2011,33(20):2120-2123.

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更新日期/Last Update: 2011-10-17