[1]胡良安,李岱容,罗永艾,等.肺结核患者外周血中CD4+CD25+FoxP3+调节T细胞增多并抑制抗结核免疫[J].第三军医大学学报,2011,33(20):2124-2127.
 Hu Liangan,Li Dairong,Luo Yongai,et al.Peripheral blood CD4+CD25+FoxP3+ regulatory T cells in active pulmonary tuberculosis patients[J].J Third Mil Med Univ,2011,33(20):2124-2127.
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肺结核患者外周血中CD4+CD25+FoxP3+调节T细胞增多并抑制抗结核免疫(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第20期
页码:
2124-2127
栏目:
论著
出版日期:
2011-10-30

文章信息/Info

Title:
Peripheral blood CD4+CD25+FoxP3+ regulatory T cells in active pulmonary tuberculosis patients
作者:
胡良安李岱容罗永艾杨相梅黄习臣
重庆医科大学附属第一医院肺科
Author(s):
Hu Liang’an Li Dairong Luo Yong’ai Yang XiangmeiHuang Xichen
Department of Lung Medicine, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
T淋巴细胞CD4+CD25+FoxP3+调节T细胞肺结核 分枝杆菌 结核免疫
Keywords:
T-lymphocytesCD4+CD25+FoxP3+regulatory T cells pulmonary tuberculosis Mycobacterium tuberculosisimmunity
分类号:
R521.02;R392.11;R392.32
文献标志码:
A
摘要:
目的      研究肺结核患者外周血中CD4+CD25+FoxP3+调节T细胞是否增多,这些调节T细胞是否抑制结核的特异细胞免疫反应。      方法      使用细胞分离、流式细胞分析及细胞增殖及增殖抑制等实验方法,对20例肺结核患者及17例健康人群外周血中CD4+CD25+FoxP3+调节T细胞的数量及免疫学特征作研究。      结果      肺结核患者外周血中CD4+CD25+FoxP3+调节T细胞数占CD4+细胞总数的比例显著高于健康人群(P<0.01)。在体外,肺结核患者外周血单个核细胞在BCG及ESAT-6的刺激下增殖能力比健康对照人群明显增强(P<0.01),产生的γ-干扰素比健康人群也明显增强(P<0.05);清除调节T细胞后的肺结核患者外周血单个核细胞对BCG刺激下产生γ-干扰素及白介素-10比没有清除时显著增强(P<0.05);从肺结核患者外周血分离CD4+CD25+调节T细胞和CD4+CD25-细胞,CD4+CD25-细胞对BCG及ESAT-6刺激产生γ-干扰素和白介素-10的能力因CD4+CD25+调节T细胞加入后而显著减弱(P<0.05)。       结论      肺结核患者CD4+CD25+FoxP3+调节T细胞增多,通过抑制结核患者细胞免疫反应,参与肺结核的发病。
Abstract:
Objective      To determine whether CD4+CD25+FoxP3+regulatory T cells(Treg) are increased in patients with active pulmonary tuberculosis(TB) and whether they suppress specific cellular immune responses.       Methods      The frequency and the immune function of circulating regulatory T cells were compared in 20 untreated patients with active pulmonary TB and 17 healthy matched control by using cell separation, flow cytometry analysis, proliferation assays and cytokines determination.       Results      The proportion of CD4+CD25+FoxP3+regulatory T cells within CD4+ T cells was significantly increased in the blood of active pulmonary TB patients  than heathy control subjects (P<0.01). Responsing to both Bacille Calmette Guerin (BCG) and early secretory antigenic target-6 (ESAT-6), the peripheral blood mononuclear cells (PBMCs) of pulmonary TB patient had significantly higher cellular proliferation  compared to the PBMCs from healthy donors (both P<0.01), and had higher IFN-γ production(both P<0.05). PBMCs from pulmonary TB patient had significantly higher BCG-induced IFN-γ and IL-10 production after CD4+CD25+regulatory T cells depletion  than before (both P<0.05). CD4+CD25- cells isolated from TB patients had significantly lower BCG-stimulated and ESAT-6-stimulated IFN-γ production  and IL-10 production  with CD4+CD25+ T cells from TB patients than in the cells without CD4+CD25+ T cells (both P<0.05).       Conclusion      CD4+CD25+FoxP3+ regulatory T cells are expanded in patients with active pulmonary TB, and therefore contribute to the pathogensis of human TB by suppressing specific Mycobacterium tuberculosis cellular immune responses.

参考文献/References:

胡良安, 李岱容, 罗永艾, 等. 肺结核患者外周血中CD4+CD25+FoxP3+调节T细胞增多并抑制抗结核免疫[J].第三军医大学学报,2011,33(20):2124-2127.

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更新日期/Last Update: 2011-10-17