[1]魏平,梁瑞凯,谭明红,等.2型糖尿病肾病大鼠肾脏MCP-1与氧化应激相关性研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(19):2021-2024.
 Wei Ping,Liang Ruikai,Tan Minghong,et al.Correlation on renal MCP-1 and oxidative stress in rats with type 2 diabetic nephropathy[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(19):2021-2024.
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2型糖尿病肾病大鼠肾脏MCP-1与氧化应激相关性研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第19期
页码:
2021-2024
栏目:
论著
出版日期:
2011-10-15

文章信息/Info

Title:
Correlation on renal MCP-1 and oxidative stress in rats with type 2 diabetic nephropathy
作者:
魏平梁瑞凯谭明红匡剑韧宁宁
第三军医大学西南医院内分泌科
Author(s):
Wei Ping Liang Ruikai Tan Minghong Kuang Jianren Ning Ning
Department of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
普罗布考单核细胞趋化蛋白-1核因子-κB2型糖尿病糖尿病肾病
Keywords:
Probucolmonocyte chemotactic protein-1NF-κBdiabetes mellitusdiabetic nephropathy
分类号:
R587.2; R341; R979.9
文献标志码:
A
摘要:
目的     观察2型糖尿病肾病(diabetic nephropathy,DN)大鼠肾脏氧化应激指标与单核细胞趋化蛋白-1(monocyte chemotactic protein-1, MCP-1)表达水平的相关性,并通过普罗布考早期干预来探讨抗氧化剂对糖尿病肾脏的保护机制。     方法       设立对照(N组,健康对照组)的同时,采用链脲佐菌素(STZ) 35 mg/kg体质量剂量腹腔注射,构建2型糖尿病肾病大鼠模型,将造模成功的2型糖尿病大鼠分别给予普罗布考(P组,普罗布考组)和NADPH氧化酶抑制剂(Apocynin)(A组,Apocynin组)干预。12周时,测量24 h尿蛋白、尿微量白蛋白以及FPG、Cr、BUN;然后处死观察肾脏病理及超微病理改变;免疫组化(S-P)法检测肾组织NT和MCP-1的表达量;RT-PCR半定量NF-κB mRNA表达,并作相关分析。     结果      ①P组和A组较DN组可以明显改善尿白蛋白、血Cr、BUN水平和肾脏肥大指数(P<0.05)。②DN组大鼠肾脏NT和MCP-1的表达呈明显正相关(r=0.0),且显著高于N组(P<0.05)。③P组肾脏NT、 MCP-1表达较DN组明显下降(P<0.05),但和A组无显著差异。     结论       普罗布考可能通过抑制氧化应激反应下调MCP-1过表达对肾脏的损伤,起到保护肾脏的作用。
Abstract:
Objective       To investigate the correlation between monocyte chemotactic protein-1 (MCP-1) expression and oxidative stress indices in kidney of type 2 diabetic nephropathy (DN) rat model, and through probucol early intervention to explore the protective mechanism of antioxidants on diabetic kidney.      Methods       Twenty-four male Wistar rats of 8 weeks age were randomly divided into a normal control group (n=6) and a model group (n=18). Rats of the normal control group were fed with regular diet, while rats of the model group were fed with high-fat high-sugar diet and 4 weeks later were given an intraperitoneal injection of 35 mg/kg streptozotocin (SZT). The successfully constructed type 2 diabetic nephropathy rat models were then randomly divided into a DN group (n=5), a P group (n=6) and an A group (n=6). Rats of the P group were given 61 mg/kg Probucol and high-fat high-sugar diet. Rats of the A group were given 200 mg/kg Apocynin (NAPDH oxidase inhibitor) and high-fat high-sugar diet. At week 12, the levels of 24-hour urine protein, urinary micro albumin, serum creatinine and blood urea nitrogen were measured. The rats were then sacrificed. Renal and ultrasturctural pathological changes were examined. Renal tissue NT and MCP-1 expressions were measured by immunohistochemical assay. NF-κB mRNA expression was semi-quantified by RT-PCR, and correlation analysis was performed.      Results        ①Compared with the DN group, the P group and A group had significantly lower levels of urinary micro albumin, serum creatinine, blood urea nitrogen and kidney hypertrophy index (P<0.05). ②MCP-1 expression in the DN group was positively correlated with NT expression and renal function (r=0.0); both MCP-1 and NT levels were significantly higher than those in the N group (P<0.05). ③NT and MCP-1 expressions in the P group were significantly decreased as compared with those in the DN group (P<0.05), but not significantly different from those in the A group.      Conclusion       Antioxidant may down-regulate MCP-1 expression through inhibition of oxidative stress in diabetic nephropathy and therefore protect renal function by reducing injury resulted from MCP-1 overexpression.

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更新日期/Last Update: 2011-10-12