[1]徐光旭,徐曼,耿卫朴,等.PD-L1+HeLa细胞对人外周血活化T细胞的免疫调节作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(16):1710-1713.
　Xu Guangxu,Xu Man,Geng Weipu,et al.Immunoregulatory effect of PD-L1+ HeLa cells on activated human peripheral blood T lymphocytes[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(16):1710-1713.
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PD-L1+HeLa细胞对人外周血活化T细胞的免疫调节作用(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第16期
页码:
1710-1713
栏目:
论著
出版日期:
2011-08-30

文章信息/Info

Title:
Immunoregulatory effect of PD-L1+ HeLa cells on activated human peripheral blood T lymphocytes
作者:
徐光旭; 徐曼; 耿卫朴; 陈瑜; 赵涌
重庆医科大学基础医学院病理学教研室,分子医学与肿瘤研究中心
Author(s):
Xu Guangxu;  Xu Man;  Geng Weipu;  Chen Yu;  Zhao Yong
Department of Pathology, Molecular Medicine and Tumor Research Center, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
PD-L1; T细胞; 增殖; 凋亡; IFN-γ
Keywords:
PD-L1;  T lymphocyte;  proliferation;  apoptosis;  IFN-γ
分类号:
R392.12；R392.3；R737.33
文献标志码:
A
摘要:
目的     探讨人宫颈癌细胞表达PD-L1对人外周血T细胞的免疫调节作用。     方法     采用PD-L1质粒转染人宫颈癌HeLa细胞株，将PD-L1+HeLa细胞与PHA刺激活化的人外周血T淋巴细胞混合培养，MTT法检测T细胞的增殖水平，流式细胞术分析T细胞凋亡率和CD8+/CD4+T细胞比例; ELISA检测PD-L1+HeLa细胞与PHA刺激活化的人外周血T淋巴细胞混合培养上清液IFN-γ的含量。     结果     MTT检测显示与PD-L1+HeLa混合培养组的T细胞增殖光密度值为（0.31±0.02），明显低于与PD-L1-HeLa混合培养组的T细胞和对照组T细胞，分别为[（0.62±0.02）和（0.59±0.03），P<0.01]；T细胞凋亡率明显高于与PD-L1-HeLa混合培养组和对照组T细胞，分别为(32.7%、17.9%和18.3%)； CD8+/CD4+T细胞比值稍低于PD-L1-HeLa混合培养组和对照组T细胞(分别为0.86、0.91和0.89)；与PD-L1+HeLa混合的T细胞培养上清液IFN-γ的含量低于与PD-L1-HeLa混合培养组和对照组T细胞，分别为[(801.1±20.5) pg/ml、(1 006.2±30.2 )pg/ml和(1 070.2±113.3) pg/ml,P<0.01]。     结论     PD-L1明显抑制人外周血活化T细胞增殖促进其凋亡并下调其免疫功能。
Abstract:
Objective     To study the immunoregulatory effect of PD-L1 expressed by human cervical carcinoma cell line HeLa on activated human peripheral blood T lymphocytes in vitro.      Methods     HeLa cells were transfected with PD-L1+ plasmid, and then the cells (PD-L1+ HeLa cells) were co-cultured with PHA-activated human peripheral blood T lymphocytes. The proliferation level of T lymphocytes was determined by MTT assay. The apoptosis rate of T lymphocytes and the ratio of CD8+ T lymphocytes to CD4+ T lymphocytes (CD8+T/CD4+T) were analyzed by flow cytometry. The IFN-γ concentration in the culture supernatant of T lymphocytes was measured by ELISA.      Results     The optical density (OD) of T lymphocytes co-cultured with PD-L1+ HeLa cells (0.31 ±0.02) was significantly lower than those of T lymphocytes co-cultured with HeLa cells free of PD-L1 (PD-L1- HeLa cells) (0.62±0.02) and T lymphocytes cultured alone (0.59±0.03) (P<0.01). The apoptosis rate of T lymphocytes co-cultured with PD-L1+ HeLa cells (32.7%) was significantly higher than those of T lymphocytes co-cultured with PD-L1- HeLa cells (17.9%) and T lymphocytes cultured alone (18.3%). The CD8+T/CD4+T ratio of T lymphocytes co-cultured with PD-L1+ HeLa cells (0.86) was slightly lower than those of T lymphocytes co-culture with PD-L1- HeLa cells (0.91) and T lymphocytes cultured alone (0.89). The IFN-γ concentration in the culture supernatant of T lymphocytes co-cultured with PD-L1+ HeLa cells (801.1±20.5 pg/ml) was significantly lower than those in the T lymphocytes co-cultured with PD-L1-HeLa cells (1006.2±30.2 pg/ml) and T lymphocytes cultured alone (1070.2±113.3 pg/ml) (P<0.01).      Conclusion     PD-L1 significantly inhibits the proliferation in active peripheral blood T lymphocytes, promotes their apoptosis, and down-regulates their immune response.

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更新日期/Last Update: 2011-08-30