[1]杨孟雪,甘华,沈清,等.1, 25-(OH)2D3及LPS对2型糖尿病肾病尿毒症患者单核细胞维生素D受体表达的影响[J].第三军医大学学报,2011,33(16):1731-1735.
 Yang Mengxue,Gang Hua,Sheng Qing,et al.Effect of 1,25-(OH)2D3 and LPS on expression of vitamin D receptor in human monocytes incubated with sera from type 2 diabetes patients and diabetic nephropathy patients with uremia[J].J Third Mil Med Univ,2011,33(16):1731-1735.
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1, 25-(OH)2D3及LPS对2型糖尿病肾病尿毒症患者单核细胞维生素D受体表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第16期
页码:
1731-1735
栏目:
论著
出版日期:
2011-08-30

文章信息/Info

Title:
Effect of 1,25-(OH)2D3 and LPS on expression of vitamin D receptor in human monocytes incubated with sera from type 2 diabetes patients and diabetic nephropathy patients with uremia
作者:
杨孟雪甘华沈清陈丹燕汤为学杨梅
重庆医科大学附属第一医院:肾内科,重庆市神经病学重点实验室;遵义医学院内分泌科
Author(s):
Yang Mengxue Gang Hua  Sheng Qing Chen Danyan Tang Weixue Yang Mei
Department of Nephrology,Key Laboratory of Neurology of Chongqing,First Affilitated Hospital, Chongqing Medical University, Chongqing, 400016;Department of Endocrinology, First Affilitated Hospital, Zunyi Medical College, Zunyi, Guizhon Province 563003, China
关键词:
2型糖尿病糖尿病肾病尿毒症125-(OH)2D3脂多糖维生素D受体
Keywords:
type 2 diabetes diabetic nephropathy uremia 125-(OH)2D3  lipopolysaccharide vitamin D receptor
分类号:
R587.1;R392.12;R977.24
文献标志码:
A
摘要:
目的     探讨1,25-(OH)2D3及TLR4配体(脂多糖,LPS)对2型糖尿病(T2DM)和糖尿病肾病(diabetic nephropathy, DN)尿毒症患者血清干预的单核细胞维生素D受体(VDR)表达的影响,进一步探索1,25-(OH)2D3在T2DM和DN炎症性免疫反应中的作用。     方法     分离研究对象(健康对照组、T2DM组和DN尿毒症组)外周血血清,孵育THP-1单核细胞,然后于含或不含10-7 mol/L的1,25-(OH)2D3培养液中培养48 h后,再用终浓度为1 μg/ml的 LPS干预24 h,收集单核细胞和培养上清。采用RT-PCR检测VDR mRNA表达,Western blot、免疫荧光检测THP-1单核细胞内VDR蛋白表达。ELISA法检测细胞培养上清IL-6和IL-10浓度。     结果     与正常对照组比较,在LPS的刺激下T2DM组和DN尿毒症组THP-1单核细胞内VDR mRNA水平下调[对照组(0.99±0.25);T2DM组(0.65±0.24);DN尿毒症组(0.62±0.27),P<0.05];DN尿毒症组THP-1单核细胞内VDR蛋白表达比正常对照组和T2DM组显著下调[对照组(0.48±0.05);T2DM组(0.50±0.06);DN尿毒症组(0.20±0.01),P<0.01],且LPS增强以上患者血清孵育的THP-1单核细胞炎症细胞因子IL-6的分泌[对照组(15.13±1.61);T2DM组(24.06±2.92);DN尿毒症组(70.77±5.48),P<0.05];而1,25-(OH)2D3可部分阻断上述作用。     结论     LPS能下调T2DM和DN尿毒症患者单核细胞VDR mRNA和蛋白的表达,引起促炎和抗炎细胞因子失调。1,25-(OH)2D3可部分逆转LPS的作用,对T2DM和DN尿毒症可能具有一定的保护作用。
Abstract:
Objective     To investigate the effects of 1,25-(OH)2D3 and TLR4 ligand lipopolysaccharide (LPS) on the expression of vitamin D receptor (VDR) in THP-1 monocytes incubated with the sera from type 2 diabetes mellitus (T2DM) patients and diabetic nephropathy (DN) patients with uremia, and to explore the role of 1,25-(OH)2D3 in the inflammatory immune response of T2DM and DN.      Methods     Peripheral blood sera of the subjects in healthy control group, T2DM group, and DN uremia group were isolated. THP-1 monocytes were incubated with the sera separately, cultured in media with or without 10-7 mol/L 1,25-(OH)2D3 for 48 h, and treated with 1 μg/ml LPS for 24 h. The monocytes and culture supernatant were collected. The mRNA and protein expression of VDR in THP-1 monocytes was evaluated by RT-PCR as well as Western blotting and immunofluorescence assay. ELISA was used to measure the IL-6 and IL-10 contents in the culture supernatant.      Results     With LPS treatment, T2DM group and DN uremia group showed down-regulated VDR mRNA expression in THP-1 monocytes as compared with the healthy control group (healthy control group 0.99±0.25; T2DM group 0.65±0.24; DN uremia group 0.62±0.27; P<0.05), and DN uremia group showed significantly down-regulated VDR protein expression in THP-1 monocytes as compared with the healthy control group and T2DM group (healthy control group 0.48±0.05; T2DM group 0.50±0.06; DN uremia group 0.20±0.01; P<0.01). LPS enhanced IL-6 secretion of THP-1 monocytes in the T2DM group and DN uremia group as compared with the healthy control group (healthy control group 15.13±1.61; T2DM group 24.06±2.92; DN uremia group 70.77±5.48; P<0.05). The above effects of LPS were partially blocked by 1,25-(OH)2D3.      Conclusion     LPS can down-regulate the mRNA and protein expression of VDR in monocytes in T2DM patients and DN patients with uremia, and cause pro-inflammatory and anti-inflammatory cytokine disorder. The effects of LPS can be partially reversed by 1,25-(OH)2D3, indicating the protective effects of 1,25-(OH)2D3 on T2DM patients and DN patients with uremia.

参考文献/References:

杨孟雪, 甘华, 沈清, 等. 1, 25-(OH)2D3及LPS对2型糖尿病肾病尿毒症患者单核细胞维生素D受体表达的影响[J].第三军医大学学报,2011,33(16):1731-1735.

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更新日期/Last Update: 2011-08-30