[1]方针强,刘永亮,王祥卫,等.hBMP-7修饰的骨髓间充质干细胞对肾脏BMP-7的影响[J].第三军医大学学报,2011,33(15):1590-1593.
 Fang Zhenqiang,Liu Yongliang,Wang Xiangwei,et al.hBMP-7-modified bone marrow mesenchymal stem cells attenuate kidney ischemia-reperfusion injury in rabbits[J].J Third Mil Med Univ,2011,33(15):1590-1593.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第15期
页码:
1590-1593
栏目:
论著
出版日期:
2011-08-15

文章信息/Info

Title:
hBMP-7-modified bone marrow mesenchymal stem cells attenuate kidney ischemia-reperfusion injury in rabbits
作者:
方针强刘永亮王祥卫易善红贾维胜陈伟何凡沈崇星叶钢
第三军医大学新桥医院泌尿外科,全军肾脏病中心
Author(s):
Fang Zhenqiang Liu Yongliang Wang Xiangwei Yi Shanhong Jia Weisheng Chen Wei He Fan Shen Chongxing Ye Gang
Center of Renal Diseases, Department of Urinary Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
骨形态发生蛋白-7骨髓间充质干细胞肾脏缺血再灌注损伤移植
Keywords:
bone morphogenetic protein-7 bone marrow mesenchymal stem cells kidney ischemia-reperfusion injury transplantation
分类号:
R692; R364.12; R394.2
文献标志码:
A
摘要:
目的     观察人骨形态发生蛋白-7(hBMP-7)基因修饰的骨髓间充质干细胞(BM-MSCs)移植对缺血再灌注损伤(ischemia-reperfusion injury, IRI)肾脏内源和外源BMP-7表达的影响,探讨其对IRI肾脏的保护作用。     方法      45只兔采用随机数字表的方法分为对照组(Ⅰ组)、单纯BM-MSCs移植组(Ⅱ组)和基因修饰的BM-MSCs移植组(Ⅲ组)(n=15)。构建hBMP-7重组腺病毒并转染兔BM-MSCs,建立肾脏IRI模型后,移植组于肾血流恢复后经肾动脉推注单纯和基因修饰的自体BM-MSCs,对照组同法推注等量生理盐水。3组分别于术后3、7、14 d采用随机数字表的方法取5只兔经耳缘静脉采血,检测血清肌酐(Crea)和尿素(Urea),观察肾功能情况;同时取肾组织进行HE染色行肾小管损伤评分,ELISA和RT-PCR法分别检测肾脏hBMP-7和BMP-7的蛋白浓度和mRNA表达。      结果      肾脏IRI后3、7、14 d,Ⅲ组Crea和Urea明显低于Ⅰ组和Ⅱ组(P<0.05);Ⅲ组肾小管损伤评分均低于Ⅰ组和Ⅱ组(P<0.05);Ⅲ组检测到第3、7、14天hBMP-7蛋白[(81.8±25.5)、(107.8±33.4)、(49.6±17.8)pg/mg]和mRNA(0.82±0.12、0.69±0.13、0.42±0.10)的表达并维持至少2周;Ⅲ组第3、7、14天BMP-7 mRNA(1.08±0.33、1.36±0.17、1.64±0.21)和BMP-7蛋白(95.9±21.3、137.0±14.9、192.1±18.0)的表达均高于Ⅰ组和Ⅱ组(P<0.05)。      结论      hBMP-7基因修饰的BM-MSCs移植既能携带外源性hBMP-7在缺血再灌注损伤肾脏中表达,又能提高内源性BMP-7的表达,减轻肾脏损伤,促进肾脏修复,改善肾脏功能。
Abstract:
Objective      To determine the effect of  transplantaiton of human bone morphogenetic protein-7 (BMP-7) expressed bone marrow mesenchymal stem cells (BM-MSCs) on the expression of endogenous and exogenous hBMP-7 in the kidney after ischemia-reperfusion injury (IRI) , and evaluate the protective effects of hBMP-7-expressed BM-MSCs transplantation.       Methods       A total of 45 rabbits were divided randomly into three groups (n=15): control group, BM-MSCs transplantation group and hBMP-7-expressed BM-MSCs transplantation group. After the establishment of kidney IRI in the left kidney, the animals received an infusion via renal artery of 1 ml normal saline, BM-MSCs or hBMP-7-expressed BM-MSCs (2×107). Blood samples and kidney was taken at the time points of 3, 7 and 14 d after the injury. Serum levels of creatinine and urea were detected to evaluate renal function. The damage of renal tubules was evaluated by HE staining. The expression of BMP-7 and hBMP-7 were detected with ELISA and RT-PCR methods.      Results       Serum levels of creatinine and urea were significantly lower in the group injected with hBMP-7-expressed BM-MSCs than in the other 2 groups (P<0.05), so did the damage in renal tubules at the 3 time points (P<0.05). The protein level of hBMP-7 was 81.8±25.5, 107.8±33.4 and 49.6±17.8 pg/mg respectively in the group at the 3 time points, while that of mRNA was 0.82±0.12, 0.69±0.13 and 0.42±0.10 respectively, both of them were detected for 2 weeks. The protein level of BMP-7 in the group was 95.9±21.3,137.0±14.9, and 192.1±18.0 in the group and that of mRNA was 1.08±0.33, 1.36±0.17 and 1.64±0.21 at the 3 time points, both of which were higher than those in the other group (P<0.05).      Conclusion       Not only can hBMP-7-expressed BM-MSCs take exogenous hBMP-7 to IRI kidney, but also improve the expression of endogenous BMP-7, thereby, attenuate the renal damage and promote the renal functional repair.

参考文献/References:

方针强, 刘永亮, 王祥卫, 等. hBMP-7修饰的骨髓间充质干细胞对肾脏BMP-7的影响[J].第三军医大学学报,2011,33(15):1590-1593.

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更新日期/Last Update: 2011-07-21