[1]彭慧霞,潘艳艳,邢兰瑛,等.槲皮素联合顺铂对人卵巢癌SKOV-3细胞增殖及凋亡的影响[J].第三军医大学学报,2011,33(15):1606-1609.
 Peng Huixia,Pan Yanyan,Xing Lanying,et al.Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells[J].J Third Mil Med Univ,2011,33(15):1606-1609.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第15期
页码:
1606-1609
栏目:
论著
出版日期:
2011-08-15

文章信息/Info

Title:
Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells
作者:
彭慧霞潘艳艳邢兰瑛王艳华蔡东阁杨雪梅李虹
西安交通大学医学院第二附属医院妇产科;陕西省妇幼保健院超声科
Author(s):
Peng Huixia Pan Yanyan Xing Lanying Wang Yanhua Cai Dongge Yang Xuemei Li Hong
Department of Gynaecology and Obstetrics, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province,710004;Maternal and Child Health Hospital of Shaanxi Province, Xi’an, Shaanxi Province, 710003, China
关键词:
槲皮素顺铂卵巢癌增殖凋亡
Keywords:
quercetin cisplatin ovarian cancer proliferation apoptosis
分类号:
R737-31; R73-361; R979.11
文献标志码:
A
摘要:
目的     探讨槲皮素单独及联合顺铂对人卵巢癌SKOV-3细胞增殖与凋亡的影响,并初步探讨其作用机制。     方法      选取对数生长期的卵巢癌SKOV-3细胞作为研究对象,实验分6组:阴性对照组(生理盐水),槲皮素低(25 μmol/L)、中(50 μmol/L)、高剂量组(100 μmol/L),顺铂组(10 μmol/L),联合组(槲皮素50 μmol/L+顺铂10 μmol/L)。采用MTT法检测不同浓度槲皮素单独及联合顺铂对SKOV-3细胞的增殖抑制作用;流式细胞仪检测细胞周期分布及细胞凋亡率;免疫细胞化学法检测槲皮素作用于SKOV-3细胞Bcl-2及MtP53蛋白表达水平的变化。     结果      与阴性对照组相比,槲皮素可明显抑制SKOV-3细胞的增殖,并具有显著的时间和剂量依赖关系,槲皮素高剂量组在72 h的抑制率可达63.49%(P<0.05);流式细胞仪分析发现槲皮素可使G0/G1期细胞明显增多,S期细胞明显减少(P<0.05),提示其将细胞周期阻滞于G1期;槲皮素还可明显诱导SKOV-3细胞凋亡,高剂量组的凋亡率可达29.51%;槲皮素作用后,Bcl-2及MtP53表达则明显降低(P<0.05)。     结论      槲皮素在体外可抑制SKOV-3细胞增殖,阻滞细胞周期,诱导细胞凋亡,其机制可能与降低凋亡相关蛋白MtP53和Bcl-2的蛋白表达水平有关;槲皮素与顺铂联用有协同作用。
Abstract:
Objective      To determine the effect of quercetin combined with cisplatin on the proliferation and apoptosis in SKOV-3 ovarian cancer cells.      Methods      Ovarian cancer SKOV-3 cells at the logarithmic growth phase were treated with quercetin at 25, 50, or 100 μmol/L respectively, with 10 μmol/L DDP, or with 50 μmol/L quercetin and 10 μmol/L DDP together, respectively for 24, 48 and 72 h. The cell proliferation was evaluated by MTT assay, the rate of cell apoptosis and the cell-cycle distribution was detected by flow cytometry, and the expression of P53 and Bcl-2 were analyzed by immunocytochemical assay.      Results        Quercetin resulted in an decreased of the proliferation in SKOV-3 cells in a dose- and time-dependent manner, and the inhibitory rate in the 100 μmol/L quercetin for 72 h was 63.49% (P<0.05). Flow cytometry showed that the proportions of G0/G1 phase and of S phase were reduced after quercetin exposure(P<0.05). Quercetin also increased the apoptotic rate of SKOV-3 cells significantly and the highest apoptotic rate was 29.51%(P<0.05). Immunocytochemical assay showed that the expression of MtP53 and Bcl-2 proteins in the treated cells was significantly decreased compared with the control(P<0.05).      Conclusion      Quercetin, especially combined with cisplatin, significantly inhibits the proliferation and induces apoptosis of cervical cancer cells, which might be due to the down-regulation of Bcl-2 and MtP53.

参考文献/References:

彭慧霞, 潘艳艳, 邢兰瑛, 等. 槲皮素联合顺铂对人卵巢癌SKOV-3细胞增殖及凋亡的影响[J].第三军医大学学报,2011,33(15):1606-1609.

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更新日期/Last Update: 2011-07-21