[1]赵海霞,牟东,薛世祥,等.结直肠癌组织中核干因子和增殖细胞核抗原的表达与血清叶酸水平的关系[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):995-997.
　Zhao Haixia,Mu Dong,Xue Shixiang,et al.Correlation between expression of nucleostemin and proliferating cell nuclear antigen in colorectal cancer tissues and serum folic acid concentrations[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):995-997.
点击复制

结直肠癌组织中核干因子和增殖细胞核抗原的表达与血清叶酸水平的关系(/HTML )
分享到：

陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第10期
页码:
995-997
栏目:
论著
出版日期:
2011-05-30

文章信息/Info

Title:
Correlation between expression of nucleostemin and proliferating cell nuclear antigen in colorectal cancer tissues and serum folic acid concentrations
作者:
赵海霞; 牟东; 薛世祥; 代剑华; 袁月; 彭贵勇; 房殿春
第三军医大学西南医院全军消化病研究所
Author(s):
Zhao Haixia;  Mu Dong;  Xue Shixiang;  Dai Jianhua;  Yuan Yue;  Peng Guiyong;  Fang Dianchun
Institute of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
核干因子; 增殖细胞核抗原; 血清叶酸; 结直肠癌
Keywords:
nucleostemin; proliferating cell nuclear antigen; serum folic acid concentrations;  colorectal cancer
分类号:
R735.34;R735.35;R730.2
文献标志码:
A
摘要:
目的    探讨核干因子(nucleostemin, NS)和增殖细胞核抗原（proliferating cell nuclear antigen, PCNA)在结直肠癌组织中的表达与血清叶酸水平的关系。    方法    2009年3-12月在肠镜下收集50例结直肠癌患者的组织，根据国际结直肠癌的病理分类标准分为高分化组(12例)、中分化组（17例）、低分化组(21例)和正常肠黏膜组(50例)，同时取受检者静脉血5 ml；采用免疫组织化学法检测NS和PCNA结直肠癌组织和正常肠黏膜的表达，采用磁性颗粒化学发光免疫法检测血清叶酸水平。    结果    NS和PCNA在结直肠癌阳性表达率分别是70.0%（35/50）和72.0%(36/50）,两者在正常肠黏膜中未见表达。NS和PCNA在高分化结直肠癌阳性率为41.7%(5/12）和33.3%(4/12)，分别显著低于中分化结直肠癌阳性率[76.5%(13/17)和82.4%（14/17）]与低分化结直肠癌阳性率[81.0%(17/21)和85.7%(18/21）,P<0.05]。患者血清叶酸水平在结直肠癌NS和PCNA阳性组与阴性组无显著差别 (P>0.05)。    结论    NS和PCNA在结直肠癌组织中有表达，且与结直肠癌的恶性程度密切相关,但血清叶酸水平与NS和PCNA在结直肠癌组织中的表达无关。
Abstract:
Objective    To investigate the correlation between the expression of nucleostemin (NS) and proliferating cell nuclear antigen (PCNA) in colorectal cancer tissues and serum folic acid concentrations.     Methods    From March to December 2009, colorectal cancer tissues were collected from 50 patients through colonoscopy. According to international colorectal cancer classification standard for the pathological, which divided into well-differentiated group(n=12), moderately differentiated (n=17), poorly differentiated(n=21)and corresponding normal intestinal mucosa tissues. At the same time, venous blood of 5 ml was collected from each of the above patients. Immunohistochemistry was used to detect the expression of NS and PCNA in colorectal cancer tissues and normal intestinal mucosa tissues. Serum folic acid concentrations were determined with magnetic chemiluminescence immunoassay.     Results    The positive expression rates of NS and PCNA in colorectal cancer tissues were 70% (35/50) and 72% (36/50), while neither of NS and PCNA expression was detected in the normal intestinal mucosa tissues. The positive expression rates of NS and PCNA in well differentiated colorectal cancer tissues were 41.7% (5/12) and 33.3% (4/12), which were significantly lower than those [76.5%(13/17) and 82.4%（14/17）] in the  moderately differentiated colorectal cancer tissues and [81.0% (17/21) and 85.7% (18/21)] in the poorly differentiated colorectal cancer tissues (P<0.05). There was no significant difference in serum folic acid concentration between NS and PCNA positive expression group and negative expression group (P>0.05).     Conclusion    NS and PCNA are expressed in colorectal cancer tissues, and their expression levels are correlated with the malignant degree of colorectal cancer. Serum folic acid concentration is not related to NS and PCNA expression in colorectal cancer.

相似文献/References:

[1]张冬,文利,邹利光,等.星形细胞瘤CT动态扫描指标与PCNA表达的关系[J].陆军军医大学学报(原第三军医大学学报),2006,28(01):15.
[2]王立媛,杨贺庆,安友仲.联合应用谷氨酰胺和L-精氨酸对饥饿大鼠肠屏障作用的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):272.
[3]赖晃文,赖声礼,杨传红,等.低功率毫米波辐照对HL60细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):831.
　LAI Huang-wen,LAI Sheng li,YANG Chuang-hong,et al.Low power millimeter waves irradiation improves proliferation of leukemia cells HL60[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):831.
[4]陈彦平,杨凯成,梁玉林,等.PPARγ和PCNA在口腔鳞癌组织的表达及临床意义[J].陆军军医大学学报(原第三军医大学学报),2011,33(16):1718.
　Chen Yanping,Yang Kaicheng,Liang Yulin,et al.Upregulation of PPARγ and PCNA in oral squamous cell carcinoma tissue and its clinical significance[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):1718.
[5]代远斌,曾希志,向志.人类大肠癌SSTR2 mRNA表达及其与细胞增殖相关性研究[J].陆军军医大学学报(原第三军医大学学报),2005,27(23):2363.
[6]王莉,刘风花,刘若男.新辅助化疗对宫颈鳞癌细胞增殖与凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2005,27(15):1599.
[7]刁鑫伟,叶明福,张哉根,等.前列腺癌AR表达与细胞增殖的关系[J].陆军军医大学学报(原第三军医大学学报),2005,27(17):1799.
[8]唐伟,李家兵.HSP70和HSP90α在人膀胱癌中的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2005,27(06):560.
[9]张建忠,周政,刘俊,等.NF-κB在兔蛛网膜下腔出血模型基底动脉中的表达及其与血管平滑肌增殖的关系[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1976.
　Zhang Jianzhong,Zhou Zheng,Liu Jun,et al.Relationship of NF-κB expression with vascular proliferation in basilar artery of subarachnoid hemorrhage rabbits[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(10):1976.
[10]丁雅明,方廖琼,周兰,等.不同孕期小鼠羊水对小鼠肝癌细胞H22增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1136.
　DING Ya-ming,FANG Liao-qiong,ZHOU Lan,et al.Effects of mouse amniotic fluid collected on different gestational days on proliferation and apoptosis of H22 cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):1136.

更新日期/Last Update: 2011-05-12