[1]崔馨,贺翔鸽,谢琳,等.Oligo基因芯片筛选视网膜慢性高眼压损伤差异表达基因的初步数据分析[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1333-1337.
　Cui Xin,He Xiangge,Xie Lin,et al.Differential expression in retina of chronic high intraocular pressure SD rats by large-scale Oligo microarray[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1333-1337.
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第13期
页码:
1333-1337
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Differential expression in retina of chronic high intraocular pressure SD rats by large-scale Oligo microarray
作者:
崔馨; 贺翔鸽; 谢琳; 葛成国; 喻红
第三军医大学大坪医院野战外科研究所：眼科，泌尿外科
Author(s):
Cui Xin;  He Xiangge;  Xie Lin; Ge Chengguo;  Yu Hong
Department of Ophthalmology,Department of Urinary Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
基因芯片; 视网膜; 慢性高眼压; 数据分析
Keywords:
gene chip; retina; chronic high intraocular pressure; data analysis
分类号:
R774.1;R446.9;R394.3
文献标志码:
A
摘要:
目的    采用35 000点的高密度Oligo基因芯片对大鼠慢性高眼压导致视网膜损害的6个时间点(7、35、60、90、180、360 d)的基因表达情况加以研究，探讨慢性高眼压影响下视网膜基因表达改变的情况。    方法      将基因分成“活动”基因和“差异”基因：在“活动”基因中，要求6个时间点中最大Ratio值/最小Ratio值的比值大于2。将差异基因再分为高、低表达及过表达基因，每一类基因按照其生物学功能进行功能检索、分组。对与轴突生长、导向、突触形成相关基因的功能进行初步探讨。    结果      差异表达基因1 692个：高表达的基因719个(Ratio≥2),包括6个过表达基因(Ratio≥7)；低表达的基因973个(Ratio≤0.5)。719个高表达基因中，功能完全未知者占54%，其余功能已知或部分已知者共分为14组，在损伤过程中所占比例最大的是代谢和物质转运组。轴突生长、导向及突触形成相关的基因，抑制轴突生长的基因及神经元的迁移、轴突的生长相关基因在损伤过程中均有呈高表达。    结论      建立了大鼠慢性高眼压损伤视网膜差异基因表达库，差异表达基因在慢性高眼压视网膜损害中可能有重要的意义。
Abstract:
Objective    To determine the differentially expressed genes in the retina of SD rats with chronic high intraocular pressure (IOP) by a large-scale Oligo microarray.     Methods    After the high IOP SD rat model was established, the rats were sacrificed in 7, 35, 60, 90, 180 and 360 d respectively, and their retina was resected for total RNA. After large-scale Oligo microarray, the obtained genes were identified as “active” or “differentiated” for the subsequent analysis. Active genes were those which were expressed and had no obvious change in all above-mentioned 6 time points. “Differentiated” gene must be “active”, and the difference between the maximum and minimum of the 6 time points was greater than 2-fold change (≥2 or ≤0.5).  Among the total 35 000 genes on the gene chip, the differential genes were divided to higher expressed (Ratio≥2) and lower expressed genes (Ratio≤0.5), and were further categorized according to their functions. Some important genes facilitating to axon growth, orientation and guidance were further analyzed.     Results    There were 1 692 genes identified as differentiated, with 719 genes of high expression (Ratio ≥2), including 6 of over-expression (Ratio ≥7), and with 973 genes of low expression (Ratio≤0.5). Among the highly expressed 719 genes, there were 54% with no idea about their functions, and the left with known or partially known functions were categorized into 14 groups. During the retinal injury repairing, the genes related to protein synthesis, material transduction and metabolism were most significant, and the genes facilitating to axon growth, orientation and guidance, related to suppress axon growth and neuron migration were also significant.     Conclusion      A differential genes bank of chronic high IOP injury of SD rat retina is established, and these differential genes might play vital roles in the retinal injury induced by high IOP.

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更新日期/Last Update: 2011-06-28