[1]杨雨晗,邓缅.PTEN基因调控口腔鳞癌细胞TCA-8113增殖及凋亡[J].第三军医大学学报,2011,33(14):1484-1487.
 Yang Yuhan,Deng Mian.PTEN gene expression inhibits proliferation and promotes apoptosis in human oral squamous carcinoma cell line TCA-8113[J].J Third Mil Med Univ,2011,33(14):1484-1487.
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PTEN基因调控口腔鳞癌细胞TCA-8113增殖及凋亡(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第14期
页码:
1484-1487
栏目:
论著
出版日期:
2011-07-30

文章信息/Info

Title:
PTEN gene expression inhibits proliferation and promotes apoptosis in human oral squamous carcinoma cell line TCA-8113
作者:
杨雨晗邓缅
成都医学院生物医学系:细胞生物学和遗传学教研室,生物化学与分子生物学教研室
Author(s):
Yang Yuhan Deng Mian
Department of Cell Biology and Genetics,Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Chengdu Medical College, Chengdu, Sichuan Province, 610083, China
关键词:
口腔鳞癌PTEN增殖凋亡
Keywords:
oral squamous carcinoma PTEN proliferation apoptosis
分类号:
R739.85;R394.2;R730.23
文献标志码:
A
摘要:
目的    评估PTEN基因在人口腔鳞癌细胞系TCA-8113中的增殖抑制和促凋亡功能。    方法    利用PTEN真核表达质粒转染TCA-8113细胞,另设PBS处理作为空白组,转染pcDNA3.1质粒和Lipofectamine处理作为对照组。采用MTT和克隆形成实验检测细胞增殖情况,采用流式细胞术、TUNEL和Western blot法检测细胞凋亡。    结果    pcDNA3.1-PTEN转染TCA-8113细胞得到高效的外源性表达。克隆形成实验结果表明,空白组、Lipofectamine处理组、空载体质粒组和PTEN转染组的克隆数分别为(107.34±4.62)、(79.27±3.71)、(72.03±2.38)和(19.73±1.02),PTEN转染组抑制率为72.60%,与空载体质粒组相比,差异有统计学意义(P<0.05);MTT实验结果显示PTEN基因的表达对TCA-8113细胞的抑制率在48 h和72 h分别为18.16%和42.74%,PTEN转染组与空载体质粒组比较有统计学差异(P<0.05);流式细胞术检测结果显示,PTEN基因能引起62.12%的细胞死亡;TUNEL检测PTEN基因高表达能引起(45.67±2.31)%的细胞凋亡;Western blot检测结果表明,PTEN能抑制Bcl-2的表达并激活Bax的表达。    结论    PTEN在TCA-8113细胞中的表达能显著抑制细胞增殖,促进细胞凋亡。
Abstract:
Objective    To evaluate the effects of PTEN gene expression on inhibiting the proliferation and promoting the apoptosis of human oral squamous carcinoma cell line TCA-8113.     Methods    TCA-8113 cells were transfected with eukaryotic expression plasmid pcDNA3.1-PTEN to obtain an experimental group. Besides, TCA-8113 cells were also treated with PBS, plasmid pcDNA3.1 and Lipofectamine to obtain a blank group and two control groups (a pcDNA3.1-transfected group and a Lipofectamine-treated group). The proliferation of TCA-8113 cells was detected by MTT assay and colony-forming assay. The apoptosis of TCA-8113 cells was evaluated by flow cytometry, TUNEL assay and Western blotting.     Results    TCA-8113 cells transfected with plasmid pcDNA3.1-PTEN had high intracellular expression of PTEN. Colony-forming assay showed that the clone numbers of the blank group, Lipofectamine-treated group, pcDNA3.1-transfected group and pcDNA3.1-PTEN-transfected group were 107.34±4.62, 79.27±3.71, 72.03±2.38 and 19.73±1.02, respectively. The inhibitive rate of TCA-8113 cells in the pcDNA3.1-PTEN-transfected group was 72.60%, significantly higher than that in the pcDNA3.1-transfected group (P<0.05). MTT assay showed that the inhibitive rates of TCA-8113 cells in the pcDNA3.1-PTEN-transfected group were 18.16% and 42.74% at 48 h and 72 h after transfection, significantly higher than those in the pcDNA3.1-transfected group (P<0.05). Flow cytometry showed that the death rate of TCA-8113 cells in the pcDNA3.1-PTEN-transfected group was 62.12%, significantly higher than that in the pcDNA3.1-transfected group (P<0.05). TUNEL assay showed that the apoptosis of TCA-8113 cells in the pcDNA3.1-PTEN-transfected group was (45.67±2.31)%, significantly higher than that in the pcDNA3.1-transfected group (P<0.05). Furthermore, PTEN inhibited Bcl-2 expression and activated Bax expression, as proven by Western blotting.     Conclusion    The expression of PTEN gene can remarkably inhibit the proliferation and promote the apoptosis of TCA-8113 cells.

参考文献/References:

杨雨晗, 邓缅. PTEN基因调控口腔鳞癌细胞TCA-8113增殖及凋亡[J].第三军医大学学报,2011,33(14):1484-1487.

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更新日期/Last Update: 2011-07-12