[1]谢家印,杜佳,李梦侠,等.RNA干扰骨髓基质细胞和/或骨髓瘤细胞APE1表达对共培养骨髓瘤细胞增殖及凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):1008-1011.
 Xie Jiayin,Du Jia,Li Mengxia,et al.Effects of RNA interference inhibiting APE1 expression in bone marrow stromal cells and/or U266 cells on proliferation and apoptosis of co-cultured U266[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):1008-1011.
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RNA干扰骨髓基质细胞和/或骨髓瘤细胞APE1表达对共培养骨髓瘤细胞增殖及凋亡的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第10期
页码:
1008-1011
栏目:
论著
出版日期:
2011-05-30

文章信息/Info

Title:
Effects of RNA interference inhibiting APE1 expression in bone marrow stromal cells and/or U266 cells on proliferation and apoptosis of co-cultured U266
作者:
谢家印杜佳李梦侠卿毅关伟曾林立王东
第三军医大学大坪医院野战外科研究所肿瘤中心
Author(s):
Xie Jiayin Du Jia Li Mengxia Qing Yi Guan Wei Zeng Linli Wang Dong
Cancer Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042,China
关键词:
多发性骨髓瘤细胞凋亡APE1基因RNA干扰
Keywords:
multiple myeloma apoptosis APE1 gene RNA interference
分类号:
R733.3;R73-351;R394.2
文献标志码:
A
摘要:
目的    探讨RNA干扰骨髓基质细胞(bone marrow stromal cells,BMSCs)及骨髓瘤细胞株U266 APE1表达对共培养的U266细胞增殖、凋亡的影响。    方法    将构建的APE1 siRNA表达载体分别导入BMSCs及U266细胞中。Western blot法检测2种细胞中APE1蛋白表达;2株细胞经APE1 siRNA处理后采用Transwell插入式培养皿构建BMSCs与骨髓瘤细胞共培养模型,采用MTT法、Annexin V-PE/7-AAD双染法、RT-PCR分别检测U266细胞增殖、凋亡及细胞中IL-6/IL-8 mRNA表达水平。    结果    APE1 siRNA可明显降低BMSCs及U266细胞APE1蛋白表达,与对照组相比差异具有统计学意义(P<0.01);在APE1 siRNA同时处理BMSCs和U266细胞后的共培养体系中,U266细胞增殖抑制及细胞凋亡明显高于单一细胞APE1敲低组及APE1 siRNA未处理组(P<0.01);U266细胞中IL-6及IL-8 mRNA表达水平亦降低(P<0.01)。    结论    抑制APE1在BMSCs和/或骨髓瘤细胞株U266的表达,可明显抑制共培养体系中U266细胞的增殖活性并促进其凋亡。
Abstract:
Objective    To study the effect of RNA interference inhibiting apurinic/apyrimidinic endonuclease 1 (APE1) expression in bone marrow stromal cells (BMSCs) and myeloma cell line U266 on the proliferation and apoptosis of co-cultured U266 cells.     Methods    Constructed APE1 siRNA expression vector Ad5v-APE1 siRNA was used to transfect BMSCs and U266 cells. A co-culture system of BMSCs and U266 cells was established with Transwell insert culture dish. The co-culture system of BMSCs and U266 cells were divided into four groups: a control group (untransfected BMSCs and U266 cells), a first single treatment group (transfected BMSCs and untransfected U266 cells), a second single treatment group (untransfected BMSCs and transfected U266 cells), and a dual treatment group (transfected BMSCs and U266 cells). The expression levels of APE1 were detected by Western blotting. The proliferation, apoptosis, and mRNA expression levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) of co-cultured U266 cells were examined by MTT assay, flow cytometry with annexin V-PE/7-AAD double staining, and RT-PCR, respectively.     Results    BMSCs and U266 cells transfected with APE1 siRNA had significantly lower protein expression of APE1 than untransfected cells (P<0.01). Compared with the two single treatment groups and the control group, the proliferation inhibition and apoptosis degrees of U266 cells in the dual treatment group significantly increased (P<0.01). Compared with the control group, the mRNA expression levels of IL-6 and IL-8 in U266 cells significantly decreased in the two single treatment groups (P<0.01) and the dual treatment group (P<0.01), showing greater decrease in the dual treatment group (P<0.01).     Conclusion    Inhibiting APE1 expression in BMSCs and/or U266 cells in a co-culture system suppresses the proliferation and promotes the apoptosis of U266 cells.

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更新日期/Last Update: 2011-05-12