[1]王均,黄延风,张爱华,等.儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型[J].第三军医大学学报,2011,33(13):1408-1410.
 Wang Jun,Huang Yanfeng,Zhang Aihua,et al.Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis[J].J Third Mil Med Univ,2011,33(13):1408-1410.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第13期
页码:
1408-1410
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis
作者:
王均黄延风张爱华许红梅
重庆医科大学附属儿童医院:儿童发育疾病研究省部共建教育部重点实验室,感染消化科
Author(s):
Wang Jun Huang Yanfeng Zhang Aihua Xu Hongmei
Key Laboratory of Developmental Diseases in Childhood Cofounded by Chongqing and Ministry of Education,Department of Infection and Digestion, Children’s Hospital, Chongqing Medical University, Chongqing, 400014, China
关键词:
结核分枝杆菌多位点可变数目串联重复序列基因分型
Keywords:
Mycobacterium tuberculosis multiple locus variable number tandem repeats analysis genotyping
分类号:
R372; R378.911; R394.3
文献标志码:
A
摘要:
目的    了解重庆地区儿童结核病中结核分枝杆菌临床分离株的数目可变串联重复序列(variable number tandem repeats, VNTR)的分布特征,寻找适合的VNTR位点组合。    方法      采用多位点串联重复序列(multiple locus VNTR analysis, MLVA)分型方法,选择标化的24个VNTR位点,对101例结核分枝杆菌临床分离株DNA进行检测,结果采用BioNumerics 6.1数据库软件进行聚类分析和单位点Hunter-Gaston分辨率指数(Hunter-Gaston index, HGI)分析,并比较分析国际推荐的分组(12、15、24位点)的基因分型鉴定能力。    结果    MLVA分析结果显示24个VNTR位点在不同菌株中存在明显的多态性,101例结核分枝杆菌临床分离株可分为1个基因群83种基因型,其中69种基因型只有1个菌株,占68.32%(69/101),另有32株临床分离株表现出14种基因型,占31.68%(32/101),成簇率为17.82%; 24个VNTR位点的HGI具有较大差异(0.168~0.829),HGI能达到0.5以上的VNTR位点数有16个;24个VNTR位点进行不同的位点组合:12、15个和24个位点组合的HGI分别为0.995、0.996、0.996。    结论      重庆地区儿童结核病中结核分枝杆菌存在基因多态性,国际推荐的15个VNTR位点组合的MLVA分型方法适用于其流行病学的研究。
Abstract:
Objective    To study the distribution of the variable number tandem repeats (VNTR) in clinically isolated strains Mycobacterium tuberculosis (M. tuberculosis) from pediatrics tuberculosis in Chongqing and explore the effective loci combination of them by multiple locus VNTR analysis (MLVA).     Methods    Twenty-four standardized MLVA loci was used to type the 101 clinically isolated M. tuberculosis strains, and the results were analyzed by BioNumerics 6.1 software, which including dendrogram and Hunter-Gaston index (HGI) of 24 loci. The HGI of different loci set (12 loci, 15 loci, and 24 loci) was evaluated.     Results    Twenty-four loci showed different polymorphism. All the 101 clinical isolates were typed into one group that displaying 83 genotypes, in which 69 strains (68.32%, 69/101) were typed 69 genotypes respectively, and 32 strains (31.68%) were typed 14 genotypes which showed cluster rate of 17.82%. The HGI of 24 loci was from 0.168 to 0.829, the number of VNTR loci with HGI higher than 0.5 was 16. Three loci set displayed different polymorphism. HGI of 12 loci, 15 loci, and 24 loci set were 0.995, 0.996, and 0.996 respectively.     Conclusion    The clinical strains of M. tuberculosis isolated from pediatrics tuberculosis in Chongqing show definite polymorphism and the standardization 15 loci VNTR set of MLVA can be used in the molecular epidemiological study of these strains in Chongqing.

参考文献/References:

王均, 黄延风, 张爱华, 等. 儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型[J].第三军医大学学报,2011,33(13):1408-1410.

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更新日期/Last Update: 2011-06-29