[1]马蓉,刘建,伍丽,等.妊娠糖尿病患者胎盘组织脂联素及受体与PPAR-γ和IL-6变化的关系[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1392-1395.
　Ma Rong,Liu Jian,Wu Li,et al.Relationship of placental adiponectin and its receptors with PPAR-γ and IL-6 in pregnancies with gestational diabetes mellitus[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1392-1395.
点击复制

妊娠糖尿病患者胎盘组织脂联素及受体与PPAR-γ和IL-6变化的关系(/HTML )
分享到：

陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第13期
页码:
1392-1395
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Relationship of placental adiponectin and its receptors with PPAR-γ and IL-6 in pregnancies with gestational diabetes mellitus
作者:
马蓉; 刘建; 伍丽; 孙江川; 肖晓秋
重庆医科大学附属第二医院妇产科；重庆医科大学生命科学研究院
Author(s):
Ma Rong;  Liu Jian;  Wu Li;  Sun Jiangchuan; Xiao Xiaoqiu
Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010;Institute of Life Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
妊娠期糖尿病; 脂联素;  脂联素受体; 胰岛素抵抗; 过氧化物酶体增殖物激活受体; 白介素-6
Keywords:
gestational diabetes mellitus;  adiponectin;  adiponectin receptors;  insulin resistance;  peroxisome proliferatoR-activated receptor;  interleukin-6
分类号:
R714.256；R587.1；R363
文献标志码:
A
摘要:
目的    研究妊娠糖尿病（gestational diabetes mellitus, GDM）患者胎盘组织中脂联素(adiponectin，APN)及其受体（Adipo R）与过氧化物酶体增殖物激活受体(PPAR-γ)、白介素-6（IL-6）变化关系。    方法      GDM和糖耐量正常孕妇(CON)各20例，采用全自动生化分析仪检测血清生化指标，荧光定量PCR法检测胎盘组织APN及其受体Adipo-R1、Adipo-R2、PPAR-γ、IL-6 mRNA水平的表达，Western blot法检测胎盘组织中PPAR-γ、IL-6蛋白水平变化。    结果      ①与CON组相比，GDM组APN mRNA表达明显降低[(1.08±0.11)vs(0.68±0.09)，t=2.79,P<0.05]，Adipo-R1 mRNA表达无显著变化[（1.06±0.09）vs(1.13±0.11),t=0.48， P>0.05）]，Adipo-R2 mRNA表达明显增加[（1.03±0.11）vs(2.29±0.42),t=0.29, P<0.05）]，PPAR-γ mRNA表达明显降低 [(1.02±0.16)vs(0.61±0.09),t=2.28，P<0.05)]，IL-6 mRNA 表达明显增加 [(1.07±0.10)vs(2.64±0.43),t=3.55,P<0.05）]。②与CON组相比，GDM组患者胎盘组织中PPAR-γ蛋白表达明显降低[（1.00±0.05）vs (0.53±0.06)，t=5.31，P<0.05]，而IL-6蛋白表达明显变化明显增加[（1.00±0.05）vs （2.26±0.22），t=5.51，P<0.05]。②脂联素 mRNA和空腹胰岛素水平、IL-6 mRNA和蛋白水平表达变化均负相关（r=-0.39, r=-0.60, r=-0.70,P<0.05），与PPAR-γmRNA水平和蛋白水平变化均呈正相关（r=0.59, r=0.60,P<0.05）。    结论      GDM患者胎盘局部脂联素的变化可能参与了胰岛素抵抗的发生。GDM患者胎盘组织中脂联素的mRNA表达变化与PPAR-γ及IL-6表达有关。
Abstract:
Objective    To investigate the mRNA and protein levels of adiponectin, adiponectin receptors, PPAR-γ and IL-6 in placental tissues from pregnant women with gestational diabetes mellitus (GDM).     Methods    Pregnant women with identified GDM (n=20) and matched women with normal glucose tolerance (CON, n=20) who admitted in our department from February  to December 2010 were recruited for this study. They all received cesarean and their blood samples and placental tissues were collected. Automatic biochemical analyzer was used to examine serum biochemical parameters. Quantitative real time PCR was used to analyze placental adiponectin (APN), adiponectin receptors (Adipo-R), peroxisome proliferatoR-activated receptor γ (PPAR-γ), and interleukin-6 (IL-6) mRNA levels; Western blot assay was applied to test placental PPAR-γ and IL-6 protein levels.     Results    Compared with CON group, APN mRNA was significantly decreased in GDM group (1.08±0.11 vs 0.68±0.09, t=2.79, P<0.05), Adipo-R2 was significantly increased (1.03±0.11 vs 2.29±0.42, t=0.29, P<0.05), but that of Adipo-R1 had no change (1.06±0.09 vs 1.13±0.11, t=0.48, P>0.05). PPAR-γ mRNA and protein levels were significantly lower (1.02±0.16 vs 0.61±0.09, t=2.28; 1.00±0.05 vs 0.53±0.06, t=5.31; P<0.05). IL-6 mRNA and protein were significantly increased (1.07±0.10 vs 2.64±0.43, t=3.55; 1.00±0.05 vs 2.26±0.22, t=5.51, P<0.05). APN mRNA was negatively correlated with fasting insulin levels and IL-6 mRNA expression and protein levels (r=-0.39, r=-0.60, r=-0.70, P<0.05), but positively correlated with PPAR-γ mRNA and protein (r=0.59, r=0.60, P<0.05).     Conclusion      Local changes of adiponectin in GDM placenta may be involved in insulin resistance. These changes are positively correlated with PPAR-γ expression, but negatively with IL-6 expression.

相似文献/References:

[1]刘波,吴小翎,张霞,等.福辛普利对非酒精性脂肪性肝炎大鼠肝纤维化的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1646.
　Liu Bo,Wu Xiaoling,Zhang Xia,et al.Effects and mechanism of fosinopril on hepatic fibrosis in rats with nonalcoholic steatohepatitis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):1646.
[2]艾智华,房殿春,张忠辉.脂联素基因Gly15Gly多态性与糖尿病大血管病变的关系[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):536.
　AI Zhi-hua,FANG Dian-chun,ZHANG Zhong-hui.Association of adiponectin gene Gly15Gly polymorphism with primary macroangiopathy in diabetes mellitus[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(13):536.
[3]周静,姜政,孙伟,等.人脂联素真核表达载体的构建及其表达[J].陆军军医大学学报(原第三军医大学学报),2009,31(14):1413.
[4]庄旭东.肝硬化患者血清脂联素水平与炎性细胞因子和胰岛素抵抗的相关性研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(19):2080.
[5]杨缙,邓华聪,廖璞.老年2型糖尿病患者血清脂联素和早期动脉粥样硬化的关系[J].陆军军医大学学报(原第三军医大学学报),2010,32(04):372.
　Yang Jin,Deng Huacong,Liao Pu.Correlation between serum adiponectin and early atherosclerosis in aged patients with type 2 diabetes mellitus[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):372.
[6]郭文昀,宋耀明,黄岚,等.脂联素对TNF-α诱导的人脐静脉内皮细胞粘附因子mRNA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1876.
　GUO Wen-yun,SONG Yao-ming,HUANG Lan,et al.Influence of adiponectin on mRNA levels of adhesion factors in umbilical vein endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1876.
[7]孟凡萍,杨刚毅,李钶,等.腺病毒载体介导的siRNA对3T3-L1细胞脂联素表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(14):1312.
　MENG Fan-ping,YANG Gang-yi,LI Ke,et al.Effects of recombinant adenovirus-mediated siRNA on adiponectin expression in 3T3-L1 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(13):1312.
[8]李庆辉,杨天伦,谭碧峰,等.原发性高血压患者血清apelin水平变化及临床意义[J].陆军军医大学学报(原第三军医大学学报),2011,33(11):1197.
[9]王金霞,任建功,罗辉,等.AMPKɑ2基因多态性与2型糖尿病及血清脂联素、颈动脉粥样硬化的关系[J].陆军军医大学学报(原第三军医大学学报),2012,34(06):530.
　Wang Jinxia,Ren Jiangong,Luo Hui,et al.Relationship of AMPKɑ2 gene single nucleotide polymorphism with type 2 diabetes, serum adiponectin level and carotid atherosclerosis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):530.
[10]魏文茂,朱丽丽,罗华丽,等.双葛解酲丸对酒精性脂肪肝大鼠炎症及脂质代谢的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):609.
　Wei Wenmao,Zhu Lili,Luo Huali,et al.Effects of Shuangge Jiecheng Pill on inflammatory factors and lipid metabolism in alcoholic fatty liver rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):609.

更新日期/Last Update: 2011-06-29