[1]朱海涛,张礼均,李飞,等.模拟急进高原环境下小型猪创伤性脑出血模型的建立[J].第三军医大学学报,2011,33(09):869-873.
 Zhu Haitao,Zhang Lijun,Li Fei,et al.A model of traumatic intracerebral hemorrhage in miniature pigs exposed to simulated high altitude[J].J Third Mil Med Univ,2011,33(09):869-873.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第09期
页码:
869-873
栏目:
论著
出版日期:
2011-05-15

文章信息/Info

Title:
A model of traumatic intracerebral hemorrhage in miniature pigs exposed to simulated high altitude
作者:
朱海涛张礼均李飞邹明明袁轶恺薛兴森李兰冯华朱刚林江凯
第三军医大学西南医院神经外科,全军神经系统疾病微创诊治专科中心
Author(s):
Zhu Haitao Zhang Lijun Li Fei Zou Mingming Yuan Yikai Xue Xingsen Li Lan Feng Hua Zhu Gang Lin Jiangkai
Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
高海拔创伤性脑出血闭合性颅脑创伤小型猪动物模型
Keywords:
high altitude traumatic intracerebral hemorrhage closed brain injury miniature pigs animal model
分类号:
R-332; R651.15; R339.54
文献标志码:
A
摘要:
目的    建立模拟急进高原环境下的实验用小型猪创伤性脑出血的动物模型。     方法     按随机数字表法将29只小型猪分为高原注血组(n=11)、高原假手术组(n=4)、平原注血组(n=11)和平原假手术组(n=3)。用动物低压氧舱模拟高原环境,采用二次法缓慢注射3 ml自体动脉血于右侧基底节区建立脑出血模型,立即行头颅CT扫描,分别于注血后各时相点(2、24、72、168 h)连续监测动脉血血气、动物行为学,168 h后行脑组织大体切片并计算血肿体积,行HE、Nissl染色,了解模型建立的稳定性及可重复性。     结果     CT扫描、脑组织大体切片均显示脑内血肿形成,168 h后血肿体积明显减小(P<0.01),但高、平原两组血肿体积差异无统计学意义(P>0.05)。致伤后,在各时相点,高原注血组神经功能得分均大于平原注血组(P<0.01),且平、高原两注血组的主要血气参数值差异也均有统计学意义(P<0.01)。高原组的神经元细胞核、尼氏体的损伤及缺血改变重于平原组。     结论      本实验制备的高原创伤性脑出血模型能产生稳定的的血肿体积、明显的神经功能缺损,可操作性强、可重复性好。
Abstract:
Objective      To establish a model of traumatic intracerebral hemorrhage (tICH) in miniature pigs exposed to simulated high altitude.      Methods     Twenty-nine miniature pigs were randomly assigned to 4 groups, high altitude tICH group (n=11), plain tICH group (n=11), high altitude sham operation group (n=4), plain sham operation group (n=3). After healthy miniature pigs were raised in hypobaric chamber simulating 4 000 meter high altitude for 3 d, they were inflicted with a slow injection of 3 ml fresh autologous arterial blood into the right basal ganglia to mimic tICH. The minipigs who received same blood injection under normal plain condition served as control. CT scanning was carried out in 2 h before and immediately after tICH induction, then the cerebral samples were taken out in 168 h after tICH induction, and the hematoma volume was calculated by stereological method. Arterial blood gas were monitored in 2 h before and in 2, 24, 72 and 168 h after tICH induction. Animal behaviors were evaluated with Purdy neurological function assessment in 2 h before and in 24, 72 and 168 h after tICH induction. Morphological changes of brain tissues were examined by HE and Nissl stainings.     Results      Hematoma in the basal ganglia was successfully induced, and the volume of hematoma was distinctly diminished in 168 h (P<0.01). But there was no significant difference in the volume between high altitude tICH group and plain tICH group (P>0.05). In 24, 72 and 168 h after tICH induction, there were significant differences in blood gas analysis (P<0.01) and total neurological scores  between high altitude tICH group and plain tICH group (P<0.01). Injuries in neuron nuclei and Nissl bodies around the hematoma were more severe in high altitude tICH group than plain tICH group.     Conclusion      The model of tICH in miniature pigs exposed to simulated high altitude can produce stable hematoma volume and visible neurological deficits.

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更新日期/Last Update: 2011-05-04