[1]王永超,刘红,彭家和,等.法尼酯X受体上调肝细胞中成纤维细胞生长因子21的表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):912-915.
　Wang Yongchao,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor upregulates fibroblast growth factor 21 expression in cultured liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(09):912-915.
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第09期
页码:
912-915
栏目:
论著
出版日期:
2011-05-15

文章信息/Info

Title:
Farnesoid X receptor upregulates fibroblast growth factor 21 expression in cultured liver cells
作者:
王永超; 刘红; 彭家和; 李良鹏; 董金瑜; 张艳; 江渝
第三军医大学基础部生物化学与分子生物学教研室；第三军医大学新桥医院血液内科
Author(s):
Wang Yongchao;  Liu Hong;  Peng Jiahe;  Li Liangpeng;  Dong Jinyu;  Zhang Yan;  Jiang Yu
Department of Bioche-mistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038; Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
法尼酯X受体; 成纤维细胞生长因子21; 鹅脱氧胆酸; 肥胖
Keywords:
Farnesoid X receptor;  fibroblast growth factor21;  chenodesoxycholic acid; obesity
分类号:
R333.4; R341; R394.3
文献标志码:
A
摘要:
目的    研究法尼酯X受体（farnesoid X receptor，FXR）对成纤维细胞生长因子21（fibroblast growth factor,FGF21）表达的影响。    方法      用FXR特异性激动剂终浓度为0、50、100 μmol/L的CDCA和0、1、5 μmol/L的GW4064分别处理人肝癌细胞株HepG2和人胚胎肝细胞L02细胞后，用逆转录-聚合酶链法（RT-PCR）检测FXR特异性靶基因小异源二聚体配体（small heterodimer parterner,SHP）和FGF21 mRNA的表达变化，再用Western blot法检测FGF21蛋白表达水平的变化。     结果      用FXR特异性激动剂CDCA和GW4064刺激后，分别比较HepG2和L02细胞SHP与β-actin mRNA RT-PCR 产物光密度比值，比值均明显增高(P<0.05),反应HepG2和L02细胞内SHP mRNA水平均明显升高，表明FXR在2种细胞中被激活；分别比较HepG2和L02细胞FGF21与β-actin RT-PCR产物光密度比值以及FGF21与β-actin蛋白光密度比值，比值均明显升高（P<0.05），表明FGF21 mRNA和蛋白水平均明显升高。    结论     激活的FXR可以上调FGF21的表达。
Abstract:
Objective     To determine the effect of farnesoid X receptor (FXR) on the expression of fibroblast growth factor21 (FGF21) in human hepatoblastoma HepG2 cells and hepatocyte L02 cells.     Methods     After HepG2 cells and L02 cells were treated with FXR agonist chenodesoxycholic acid (CDCA) at 0, 50 or 100 μmol/L, or with another agonist GW4064 at 0, 1 or 5 μmol/L respectively. The FXR specific target gene small heterodimer partner (SHP) and the FGF21 mRNA level were detected by reverse transcription-polymerase chain reaction (RT-PCR), and FGF21 protein level was determined by Western blotting.     Results     After treated with CDCA and GW4064, the mRNA expression of SHP was significantly increased in the treated HepG2 and L02 cells when compared with the untreated cells respectively (P<0.05), which suggested that FXR was functionally activated in those cells. The mRNA and protein expressions of FGF21 were also increased in those treated cells (P<0.05).     Conclusion      FXR receptor upregulates the expression of FGF21 in hepatocytes.

相似文献/References:

[1]申丽丽,彭家和,刘红,等.法尼酯X受体对脂肪酸合酶表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2142.
　SHEN Li-li,PENG Jia-he,LIU Hong,et al.Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(09):2142.
[2]娄桂予,周度金.法尼酯X受体的研究进展[J].陆军军医大学学报(原第三军医大学学报),2005,27(20):2088.
[3]周婷婷,秦波,郑天鹏,等.非酒精性脂肪肝患者血浆FGF21水平及与肥胖、胰岛素抵抗关系研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(03):265.
　Zhou Tingting,Qin Bo,Zheng Tianpeng,et al.Correlation between plasma fibroblast growth factor 21 level, obesity and insulin resistance in nonalcoholic fatty liver disease patients[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(09):265.
[4]董金瑜,刘红,彭家和,等.法尼酯X受体可下调肝细胞中肝型脂肪酸结合蛋白的表达[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):458.
　Dong Jinyu,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor down-regulates expression of liver fatty acid-binding protein in liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(09):458.

更新日期/Last Update: 2011-05-04