[1]刘熙,刘刚,张妮,等.LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响[J].第三军医大学学报,2011,33(07):690-694.
 Liu Xi,Liu Gang,Zhang Ni,et al.Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca2+ uptake/release in genioglossus of rabbits exposed to chronic hypoxia[J].J Third Mil Med Univ,2011,33(07):690-694.
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LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第07期
页码:
690-694
栏目:
论著
出版日期:
2011-04-15

文章信息/Info

Title:
Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca2+ uptake/release in genioglossus of rabbits exposed to chronic hypoxia
作者:
刘熙刘刚张妮欧娜张鹏
第三军医大学西南医院呼吸内科
Author(s):
Liu Xi Liu Gang Zhang Ni Ou Na Zhang Peng
Department of Respiratory Diseases, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
缺氧睡眠呼吸暂停阻塞性电刺激颏舌肌肌球蛋白重链肌浆网Ca2+
Keywords:
anoxia sleep apnea obstructive  electric stimulation genioglossus myosin heavy chain sarcoplasmic reticulum Ca2+
分类号:
R322.74; R337.5; R364.4
文献标志码:
A
摘要:
目的    研究超低频复合生理频率慢性电刺激(lower physiological frequency chronic electrical stimulation,LPCES)对慢性低压缺氧兔颏舌肌肌球蛋白重链(myosin heavy chain,MHC)亚型表达和肌浆网(sarcoplasmic reticulum,SR)Ca2+摄取-释放动力学的影响。    方法     成年日本大白兔24只,按随机数字表法分为4组(n=6):A:正常对照组,B:慢性低压缺氧组,C:2.5 Hz电刺激组,D:(2.5+40)Hz电刺激组。B、C、D组兔在构建慢性低压缺氧模型后,给予C、D 2组慢性电刺激,刺激频率分别为超低频2.5 Hz和超低频复合生理频率(2.5+40)Hz。采用Western blot分析4组兔颏舌肌MHC亚型表达变化,采用荧光光度测定法测定SR Ca2+摄取-释放动力学。    结果     ①同正常对照组[(72.16±2.98)%]比较,慢性低压缺氧组[(80.45±1.26)%]兔颏舌肌MHCⅡa亚型比例明显增加(P<0.05),MHCⅠ亚型比例明显减少[(10.86±1.26)% vs (18.61±3.19)%,P<0.05],SR Ca2+摄取-释放速率明显下降,摄取-释放量也明显减少(P<0.05)。②同慢性低压缺氧组比较,慢性电刺激后各组兔MHCⅡa和MHCⅠ亚型比例明显增加(P<0.05),MHCⅡb亚型比例明显减少(P<0.05),(2.5+40)Hz电刺激组较2.5 Hz电刺激组更为显著(P<0.05);SR Ca2+摄取-释放速率加快,摄取-释放量增加(P<0.05),(2.5+40)Hz电刺激组较2.5 Hz电刺激组更为明显(P<0.05)。同2.5 Hz电刺激组比较,(2.5+40)Hz电刺激组MHCⅡa和MHCⅠ亚型比例差异无统计学意义(P>0.05)。    结论     慢性电刺激后慢性低压缺氧兔颏舌肌MHC呈现Ⅱb型向Ⅱa型和Ⅰ型的转变,SR Ca2+摄取-释放功能增强,其中超低频复合生理频率组变化更加明显。
Abstract:
Objective    To identify the effect of chronic electrical stimulation at a lower physiological frequency on the expressions of myosin heavy chain (MHC) isoforms and kinetics of sarcoplasmic reticulum (SR) Ca2+ uptake/release in the genioglossus of rabbits exposed to chronic hypoxia.     Methods    Twenty-four adult rabbits were randomized into control group (A), chronic hypoxia group (B), 2.5 Hz electrical stimulation group (C) and (2.5+40) Hz electrical stimulation group (low frequency plus physical frequency, D). After the rabbits from group B, C and D had been fed with free access to food and water in a hypoxia cabin (simulating 5 000 m altitude) in 10 h a day for 4 weeks, the rabbits in group C and D received electrical stimulation in their genioglossus at a frequency of 2.5 Hz and (2.5+40) Hz respectively in 10 h per day for 14 d, while those in group B received no electrical stimulation. Expressions of MHC isoforms in the genioglossus of rabbits in 4 groups were detected by Western blotting, and Fura-2 fluorophotometry was used to assay the kinetics changes of SR Ca2+ uptake-release.     Results     The expression level of MHCⅡa was significantly higher while that of MHCⅠ was significantly lower in group B than that in group A (P<0.05). Meanwhile, the genioglossus SR Ca2+ uptake/release velocity in group B was significantly decreased compared with that in group A (P<0.05). The expression levels of MHCⅡa and MHCⅠ in group C and D after electrical stimulation were significantly higher, while those of MHCⅡb, especially in group D, were significantly lower than those in group B (P<0.05). The genioglossus SR Ca2+ uptake/release velocity in group C and D, especially in group D, was significantly increased compared with that in group B (P<0.05). No significant difference was found in expression levels of MHCⅡa and MHCⅠ between group C and D after electrical stimulation (P>0.05).     Conclusion    MHCⅡb in the genioglossus of rabbits with chronic hypoxia is transformed to MHCⅡa and MHCⅠ after electrical stimulation, and SR Ca2+ uptake/release velocity is increased, especially at a lower physiological frequency of (2.5+40) Hz.

参考文献/References:

刘熙,刘刚,张妮,等. LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响[J].第三军医大学学报,2011,33(7):690-694.

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更新日期/Last Update: 2011-03-30