[1]邢晓婧,宋秋荷,钟白玉,等.mEGF乙醇脂质体的制备和特性研究[J].第三军医大学学报,2011,33(07):719-722.
 Xing Xiaojing,Song Qiuhe,Zhong Baiyu,et al.Preparation of mEGF ethosome and its properties[J].J Third Mil Med Univ,2011,33(07):719-722.
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mEGF乙醇脂质体的制备和特性研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第07期
页码:
719-722
栏目:
论著
出版日期:
2011-04-15

文章信息/Info

Title:
Preparation of mEGF ethosome and its properties
作者:
邢晓婧宋秋荷钟白玉郝飞
第三军医大学西南医院皮肤科
Author(s):
Xing Xiaojing Song Qiuhe Zhong Baiyu Hao Fei
Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
表皮生长因子乙醇脂质体高效液相色谱法原子力显微镜
Keywords:
epidermal growth factorethosome high pressure liquid chromatographyatomic force microscope
分类号:
R943; R961
文献标志码:
A
摘要:
目的    制备mEGF乙醇脂质体,并研究mEGF乙醇脂质体囊泡的包封率和物理性质。    方法     采用高效液相色谱法(HPLC)测定mEGF乙醇脂质体的包封率、精密度、回收率、稳定性,运用扫描电镜、原子力显微镜、透射电镜和激光粒度分析仪观察和分析mEGF乙醇脂质体囊泡的外观、粒径和均一度。    结果     mEGF乙醇脂质体的包封率大约为38%,精密度小于5%,30 d内包封率波动在36%~38%之间。运用原子力显微镜和透射电镜观察到粒径分布于40~60 nm 的乙醇脂质体囊泡。并且在激光动态光散射仪下测定显示囊泡的粒度分布较为均一,mEGF乙醇脂质体的折光度为4.6,囊泡粒径大约分布在100~500 nm之间。    结论     成功制备了mEGF乙醇脂质体,建立了测定包封率的色谱方法,该给药系统稳定性较好,乙醇脂质体囊泡粒径分布均匀。
Abstract:
Objective    To prepare an ethosomal delivery system containing mouse epidermal growth factor (mEGF) and investigate its entrapment efficiency and physical properties.     Methods    The entrapment efficiency of mEGF ethosome (10 μg/g) was determined by high pressure liquid chromatography (HPLC). The mEGF ethosome vesicles were visualized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and transmission electron microscopy (TEM). And the size distribution of ethosome vesicles was investigated using dynamic light scattering (DLS).     Results     The mEGF ethosome had an entrapment efficiency of 38% and the RSD was less than 5%. The entrapment efficiency of mEGF ethosomal carriers ranged from 36% to 38% during 30 d after having been prepared. mEGF ethosomal carriers had rather small vesicles in a size ranging from 40 nm to 60 nm visualized by AFM and TEM. And the size of the mEGF ethosomal delivery system had uniform distribution ranging from 100 nm to 500 nm. Also, the refractive index of the mEGF ethosomal delivery system was 4.6.     Conclusion    A mEGF ethosomal delivery system is successfully prepared. A HPLC method is developed to determine mEGF content in ethosomal delivery system. The delivery system shows a well stability and a narrow particle size distribution.

参考文献/References:

邢晓婧,宋秋荷,钟白玉,等. mEGF乙醇脂质体的制备和特性研究[J].第三军医大学学报,2011,33(7):719-722.

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更新日期/Last Update: 2011-03-30