[1]贾朝莉,吴婷婷,龙方懿,等.低氧诱导金属硫蛋白对甲状腺乳头状癌上皮间质转化的影响[J].第三军医大学学报,2011,33(07):671-674.
 Jia Chaoli,Wu Tingting,Long Fangyi,et al.Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J].J Third Mil Med Univ,2011,33(07):671-674.
点击复制

低氧诱导金属硫蛋白对甲状腺乳头状癌上皮间质转化的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第07期
页码:
671-674
栏目:
论著
出版日期:
2011-04-15

文章信息/Info

Title:
Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells
作者:
贾朝莉吴婷婷龙方懿刘智敏
重庆医科大学基础医学院生物化学与分子生物学教研室,分子医学与肿瘤研究中心
Author(s):
Jia Chaoli Wu Tingting Long Fangyi Liu Zhimin
Department of Biochemistry and Molecular Biology, Institute of Molecular Medicine and Cancer, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
关键词:
金属硫蛋白上皮间质转化低氧甲状腺乳头状癌
Keywords:
metallothioneins epithelial-mesenchymal transition hypoxia papillary thyroid carcinoma
分类号:
R736.1; R364.4; R730.2
文献标志码:
A
摘要:
目的    研究低氧诱导金属硫蛋白(metallothioneins, MTs)对甲状腺乳头状癌(papillary thyroid carcinoma,PTC)上皮间质转化(epithelial-mesenchymal transition, EMT)的影响。    方法      以人甲状腺乳头状癌NPA细胞为研究模型,将其分为正常氧组(21%O2)、低氧组(5%O2)和MTs-siRNA干扰低氧组,Western blot、RT-PCR方法检测各组细胞MTs、上皮细胞钙粘蛋白(E-cadherin)和间质细胞波形蛋白(Vimentin)的表达,Transwell/Matrigel方法检测各组细胞侵袭转移细胞数。    结果      低氧可诱导NPA细胞表达MTs,并伴随有EMT标志性分子E-cadherin表达的下调和Vimentin表达的升高,侵袭细胞数由正常氧组15.6±2.8增加到40.3±5.1,迁移细胞数由25.1±5.6增加到48.7±7.6,均有统计学差异(P<0.01);MTs靶向干扰可有效抑制低氧诱导的MTs蛋白表达,同时E-cadherin表达回升,Vimentin表达下降,侵袭细胞数仅增加到25.7±4.2,迁移细胞数仅增加到35.5±5.4,与低氧组比较均有统计学差异(P<0.05)。     结论      低氧所诱导表达的MTs对人PTC细胞发生EMT有促进作用,MTs靶向干扰可使EMT受到部分抑制。
Abstract:
Objective     To determine the effects of metallothioneins (MTs) induced by hypoxia on epithelial-mesenchymal transition (EMT) in human papillary thyroid carcinoma NPA cells.     Methods     NPA cells were divided into 3 groups, normoxia (21% O2), hypoxia (5% O2) and hypoxia with MTs-siRNA. The expression levels of MTs, E-cadherin and Vimentin in the 3 groups of NPA cells were analyzed by Western blotting and RT-PCR. The invasion and metastasis potential of these groups were detected by Transwell/Matrigel assay.     Results     Hypoxia treatment led to inducible expression of MTs, down-regulated of E-cadherin and up-regulated Vimentin at the same time, which were marker molecules of EMT in NPA cells. Meanwhile, invasive cell population of hypoxia group was increased to 40.3±5.1 from 15.6±2.8 of normoxia group (P<0.01). Migrating cell population was increased to 48.7±7.6 from 25.1±5.6 of normoxia group (P<0.01). However, the inducible expression of MTs was effectively inhibited by transfection of MTs-siRNA vector in NPA cells, followed by increased expression of E-cadherin and decreased expression of Vimentin. Moreover, invasive and migrating cell population of the hypoxia group with MTs-siRNA were only increased to 25.7±4.2 and 35.5±5.4(P<0.05).     Conclusion     The expression of MTs induced by hypoxia can promote EMT in human papillary thyroid carcinoma. siRNA targeting MTs can partially inhibit EMT promoted by hypoxia.

参考文献/References:

贾朝莉,吴婷婷,龙方懿,等. 低氧诱导金属硫蛋白对甲状腺乳头状癌上皮间质转化的影响[J].第三军医大学学报,2011,33(7):671-674.

相似文献/References:

[1]臧春宝,曹姝,李芳,等.IL-6对食管癌细胞侵袭迁移及上皮间质转化的影响[J].第三军医大学学报,2013,35(04):302.
 Zang Chunbao,Cao Shu,Li Fang,et al.Effects of interleukin-6 on invasion, migration and epithelial-mesenchymal transition in human esophageal carcinoma cells[J].J Third Mil Med Univ,2013,35(07):302.
[2]任泂,李蓉,刘晶,等.金属硫蛋白和锌对急性贫铀中毒大鼠的解毒效果研究[J].第三军医大学学报,2010,32(04):311.
 Ren Jiong,Li Rong,Liu Jing,et al.Detoxifying effect of metallothionein and zinc on acute depleted uranium poisoning in rats[J].J Third Mil Med Univ,2010,32(07):311.
[3]何颖,张竹君.IBP通过上皮间质转化促进结肠癌细胞迁移和侵袭[J].第三军医大学学报,2016,38(11):1281.
 He Ying,Zhang Zhujun.IBP promotes migration and invasion in colon cancer cells via epithelial to mesenchymal transition[J].J Third Mil Med Univ,2016,38(07):1281.
[4]贾朝莉,龙方懿,刘智敏,等.氯化钴对甲状腺乳头状癌NPA细胞上皮间质转化的影响及其机制[J].第三军医大学学报,2012,34(02):150.
 Jia Chaoli,Long Fangyi,Liu Zhimin,et al.CoCl2 improves epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J].J Third Mil Med Univ,2012,34(07):150.
[5]王峰,曹蕾,庞丽娜,等.CXCR4通过EMT促进食管癌侵袭转移过程[J].第三军医大学学报,2013,35(22):2443.
 Wang Feng,Cao Lei,Pang Lina,et al.CXCR4 participates in invasion and metastasis of esophageal carcinoma through epithelial-mesenchymal transition[J].J Third Mil Med Univ,2013,35(07):2443.
[6]宗贝歌,周艳,杨新华,等.ERβ1影响乳腺癌上皮间质转化和侵袭迁移的初步实验研究[J].第三军医大学学报,2014,36(04):317.
 Zong Beige,Zhou Yan,Yang Xinhua,et al.ERβ1 inhibits epithelial-mesenchymal transition, invasion and migration of breast cancer cells[J].J Third Mil Med Univ,2014,36(07):317.
[7]刘树刚,孔祥英,左连富,等.锌对幼鼠脑金属硫蛋白表达的影响[J].第三军医大学学报,1997,19(04):0.[doi:10.16016/j.1000-5404.1997.04.022 ]
 Liu Shugang,Kong Xiangying,Zuo Lianfu,et al.[J].J Third Mil Med Univ,1997,19(07):0.[doi:10.16016/j.1000-5404.1997.04.022 ]
[8]王五艺,程勇,万林.AGEs通过JAK2/STAT3信号通路诱导结肠癌SW480细胞增殖、侵袭及上皮间质转化[J].第三军医大学学报,2017,39(24):2390.
 WANG Wuyi,CHENG Yong,WAN Lin.Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway[J].J Third Mil Med Univ,2017,39(07):2390.
[9]王力伟,周晓洲,陈志文,等.膀胱癌循环肿瘤细胞NANOG基因的表达与临床特征相关性的初步研究[J].第三军医大学学报,2018,40(08):711.
 WANG Liwei,ZHOU Xiaozhou,CHEN Zhiwen,et al.Correlation of Nanog expression in circulating tumor cells with clinical characteristics of patients with bladder cancer[J].J Third Mil Med Univ,2018,40(07):711.
[10]隆姝孜,周寒静,刘静姝,等.mir-151a-5p对肺癌A549细胞生物学功能和化疗敏感性的影响[J].第三军医大学学报,2019,41(08):770.
 LONG Shuzi,ZHOU Hanjing,LIU Jingshu,et al.Down-regulation of microRNA-151a-5p inhibits proliferation, migration and invasion and enhances chemosensitivity of lung cancer A549 cells [J].J Third Mil Med Univ,2019,41(07):770.

更新日期/Last Update: 2011-03-30