[1]韩勇军,吴新荣.ptges3a基因在斑马鱼早期胚胎发育过程中的表达[J].第三军医大学学报,2011,33(04):389-391.
 Han Yongjun,Wu Xinrong.Expression of ptges3a during early development in zebrafish[J].J Third Mil Med Univ,2011,33(04):389-391.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第04期
页码:
389-391
栏目:
论著
出版日期:
2011-02-28

文章信息/Info

Title:
Expression of ptges3a during early development in zebrafish
作者:
韩勇军吴新荣
广州军区广州总医院药剂科
Author(s):
Han Yongjun Wu Xinrong
Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong Province, 510010, China
关键词:
斑马鱼前列腺素E合成酶胚胎发育
Keywords:
zebrafishprostaglandin E synthase 3aembryonic development
分类号:
R321;R345;R394.2
文献标志码:
A
摘要:
目的   克隆斑马鱼前列腺素E合酶3a(prostaglandin E synthase 3a, ptges3a)基因,研究其在斑马鱼胚胎早期发育中的表达情况。   方法   提取斑马鱼胚胎的总RNA,制备DIG标记的ptges3a RNA反义探针,整胚原位杂交研究ptges3a 在斑马鱼胚胎早期发育过程的表达。   结果   ptges3a在shield期前普遍性表达,10体节期在后脑神经龙骨处有特异性表达,18体节期在后脑有特异性表达,在26 hpf时期头部表达较多,并且在肾小管有特异性表达,36 hpf ptges3a在头部较高表达。   结论   ptges3a可能参与了脑部发育和肾小管形成。
Abstract:
Objective   To clone prostaglandin E synthase 3a (ptges3a) gene and investigate its temporal and spatial expression pattern in zebrafish during early development.   Methods   The total RNA of different phases of zebrafish embryos was extracted. Digoxin-labeled RNA probe of ptges3a was prepared. Whole embryo mount in situ hybridization was employed to investigate the expression pattern of ptges3a during zebrafish embryogenesis.    Results   ptges3a transcript was distributed ubiquitously before shield stage. At 10-somite there were detectable expression of ptges3a gene in the hindbrain neural keel, and at 18-somite it was expressed in the hindbrain. From 26 to 36 h after post fertilization (hpf), high expression of ptges3a was found in the brain. ptges3a was also detectable in the renal tubule at 26 hpf.   Conclusion   The expression pattern of ptges3a during zebrafish embryogenesis suggests that it may be critical for the development of brain and renal tubule. Further investigation on the function of ptges3a is needed.

参考文献/References:

韩勇军, 吴新荣. ptges3a基因在斑马鱼早期胚胎发育过程中的表达[J].第三军医大学学报,2011,33(4):389-391.

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更新日期/Last Update: 2011-02-18