[1]屈小玲,刘德峰,刘建平.AT2R基因在体电穿孔转染抑制大鼠颈总动脉新生内膜增生[J].第三军医大学学报,2011,33(06):562-565.
 Qu Xiaoling,Liu Defeng,Liu Jianping.Electroporation-mediated in vivo AT2R gene transfection inhibits neointimal hyperplasia after balloon angioplasty in rat common carotid arteries[J].J Third Mil Med Univ,2011,33(06):562-565.
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AT2R基因在体电穿孔转染抑制大鼠颈总动脉新生内膜增生(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第06期
页码:
562-565
栏目:
论著
出版日期:
2011-03-30

文章信息/Info

Title:
Electroporation-mediated in vivo AT2R gene transfection inhibits neointimal hyperplasia after balloon angioplasty in rat common carotid arteries
作者:
屈小玲刘德峰刘建平
第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所
Author(s):
Qu Xiaoling Liu Defeng Liu Jianping
Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
基因 AT2R基因转染内膜增生损伤
Keywords:
angiotensin Ⅱ type 2 receptor gene transfection neointimal hyperplasia injured common carotid artery
分类号:
R543.402;R364.31;R394.2
文献标志码:
A
摘要:
目的    探讨AngⅡ2型受体(AT2R)基因局部电穿孔转染对大鼠颈总动脉球囊损伤后新生内膜增生的影响。    方法    建立大鼠颈总动脉球囊损伤模型,用局部电穿孔方法转染AT2R真核表达载体 (pEGFP-AT2R)或空载体(pEGFP-N2),分别于术后7、14 d和21 d采用HE染色及RT-PCR方法进行AT2R、AngⅡ1型受体(AT1R)在颈动脉壁中表达的变化和组织形态学分析,检测其对在体血管新生内膜的影响作用。    结果    球囊损伤21 d后,pEGFP-AT2R组大鼠颈动脉AT2R mRNA表达为(1.262±0.317),pEGFP-N2组为(0.396±0.100),单纯损伤组为(0.410±0.053),pEGFP-AT2R组与pEGFP-N2组和单纯损伤组相比差异有统计学意义(P<0.01);球囊损伤后21 d时AT1R的表达,pEGFP-AT2R组为(0.469±0.065)、pEGFP-N2组为(0.363±0.046)、单纯损伤组为(0.373±0.045),pEGFP-AT2R组较pEGFP-N2组和单纯损伤组相比差异有统计学意义(P<0.05),pEGFP-N2组和单纯损伤组间无统计学差异(P>0.05);球囊损伤后21 d,pEGFP-AT2R组的内膜面积与中膜面积比(I/M)为(0.828±0.101),pEGFP-N2组为(1.432±0.086),单纯损伤组为(1.515±0.078),pEGFP-AT2R组较pEGFP-N2组和单纯损伤组相比差异有统计学意义(P<0.01)。pEGFP-AT2R组与单纯损伤组相比,使实验动物新生内膜增生平均受抑制率达45.35%。    结论    在体血管局部电穿孔转染AT2R基因可使AT2R在损伤血管组织表达较单纯损伤组明显增加。AT2R基因与AT1R基因之间在表达量上不存在此消彼长的关系。
Abstract:
Objective    To study the effect of electroporation-mediated in vivo local transfection of angiotensin Ⅱ type 2 receptor (AT2R) gene on neointimal hyperplasia in rat common carotid arteries after balloon angioplasty.     Methods     Seventy-two healthy SD rats were randomly and evenly divided into a control group, an injured group, an empty plasmid group (pEGFP-N2 group), and an AT2R transfection group (pEGFP-AT2R group). Balloon catheter denudation of the endothelia of rat common carotid arteries was routinely used to construct a restenosis model. The transfection of plasmids pEGFP-AT2R and pEGFP-N2 was carried out by local electroporation. The injured arteries were collected 7 d, 14 d, and 21 d after transfection. AT2R and angiotensin Ⅱ type 1 receptor (AT1R) expression levels in the carotid artery walls as well as histomorphological characteristics were analyzed with RT-PCR and HE staining. The effect of AT2R on neointimal hyperplasia of in vivo arteries was evaluated.     Results    The AT2R mRNA expression level in the injured rat common carotid arteries of the pEGFP-AT2R group was (1.262±0.317), indicating remarkable difference from those of the pEGFP-N2 group (0.396±0.100) and injured group (0.410±0.053) 21 d after transfection (P<0.01). The AT1R mRNA expression level in the pEGFP-AT2R group was 0.469±0.065, statistically higher than those of the pEGFP-N2 group (0.363±0.046) and injured group (0.373±0.045) 21 d after transfection (P<0.05). The intima-to-media (I/M) area ratio of the pEGFP-AT2R group (0.828±0.101) showed obvious difference from those of the pEGFP-N2 group (1.432±0.086) and injured group (1.515±0.078) 21 d after transfection (P<0.01). Compared with the injured group, AT2R transfection in the pEGFP-AT2R group inhibited neointimal hyperplasia in experimental animals at a rate as high as 45.35%.     Conclusion    Electroporation-mediated in vivo AT2R gene transfection can significantly up-regulate AT2R expression in injured common carotid artery tissues of rats. There is no negative correlation between the expression levels of AT2R and AT1R.

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更新日期/Last Update: 2011-03-15