[1]郭芮伶,吴国明,李海东,等.人小细胞肺癌多药耐药细胞H446/CDDP基因组DNA甲基化状态的变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(23):2533-2535.
 Guo Ruiling,Wu Guoming,Li Haidong,et al.Genomic DNA methylation in multidrug-resistant cell line H446/CDDP of human small-cell lung cancer[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(23):2533-2535.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第23期
页码:
2533-2535
栏目:
论著
出版日期:
2010-12-15

文章信息/Info

Title:
Genomic DNA methylation in multidrug-resistant cell line H446/CDDP of human small-cell lung cancer
作者:
郭芮伶吴国明李海东潘峰李文碧戢福云钱桂生
解放军第324医院呼吸内科;第三军医大学新桥医院全军呼吸内科研究所
Author(s):
Guo RuilingWu GuomingLi HaidongPan FengLi WenbiJi Fuyun Qian Guisheng
Department of Respiratory Diseases, No. 324 Hospital of PLA, Chongqing, 400020; Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
人小细胞肺癌多药耐药DNA甲基化甲基化敏感性随机引物PCR法
Keywords:
human small cell lung cancer multidrug-resistance DNA methylation methylation-sensitive arbitrarily primed PCR
分类号:
R394.2; R73-354; R734.2
文献标志码:
A
摘要:
目的    探讨基因组DNA甲基化状态与人小细胞肺癌(small cell lung cancer,SCLC)H446/CDDP细胞获得性多药耐药的关系。    方法     分别提取人SCLC多药耐药细胞H446/CDDP及其亲代细胞H446的基因组DNA,应用甲基化敏感性随机引物PCR(MS-AP-PCR)方法检测其甲基化状态。    结果     H446/CDDP细胞经酶解后的扩增谱带较H446细胞明显增多,且荧光强度较强,表明其基因组DNA甲基化水平较亲代细胞明显增高。    结论     H446/CDDP细胞多药耐药性的获得可能与其基因组DNA甲基化水平增高有关。
Abstract:
Objective    To study the correlation between genomic DNA methylation and acquired multidrug-resistance of human small cell lung cancer (SCLC) cell line H446/CDDP.     Methods     Genomic DNA was extracted from H446/CDDP cells and their parental cells H446. Genomic DNA methylation was detected by methylation-sensitive arbitrarily primed PCR (MS-AP-PCR).     Results    The amplified bands of H446/CDDP cells were significantly more than those of H446 cells and their fluorescence intensity was higher than that of H446 cells after enzymolysis, indicating that the genomic DNA methylation level is higher in H446/CDDP cells than in H446 cells.     Conclusion    The acquired multidrug-resistance of H446/CDDP cells is correlated with the high genomic DNA methylation level.

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更新日期/Last Update: 2010-12-06