[1]李砚,陈济安,赵清,等.谷胱甘肽转移酶基因多态性在微囊藻毒素致人群肝损伤发生中的作用[J].第三军医大学学报,2010,32(21):2322-2325.
 Li Yan,Chen Jian,Zhao Qing,et al.Role of glutathione S-transferase genetic polymorphisms in persons with liver damage due to microcystin exposure[J].J Third Mil Med Univ,2010,32(21):2322-2325.
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谷胱甘肽转移酶基因多态性在微囊藻毒素致人群肝损伤发生中的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第21期
页码:
2322-2325
栏目:
论著
出版日期:
2010-11-15

文章信息/Info

Title:
Role of glutathione S-transferase genetic polymorphisms in persons with liver damage due to microcystin exposure
作者:
李砚陈济安赵清邱志群许川曾慧舒为群
第三军医大学军事预防医学院环境卫生学教研室
Author(s):
Li Yan Chen Ji’an Zhao Qing Qiu Zhiqun Xu Chuan Zeng Hui Shu Weiqun
Department of Environmental Hygiene, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China
关键词:
微囊藻毒素肝损伤基因多态性谷胱甘肽转移酶M1谷胱甘肽转移酶T1
Keywords:
microcystin liver damage genetic polymorphism GSTM1 GSTT1
分类号:
R123; R394.5 R575
文献标志码:
A
摘要:
目的    探讨谷胱甘肽转移酶(glutathione S-transferase ,GST)M1(GSTM1)和T1(GSTT1)基因多态性在微囊藻毒素(microcystins,MCs)致人群肝损伤发生中的作用。    方法    在重庆涪陵区采用酶联免疫法检测饮用水和水产品中MCs浓度,并随机在MCs暴露人群中选择肝功异常人群81名,肝功正常人群92名为研究对象进行病例对照研究,多重PCR方法检测GSTM1和GSTTl基因多态性,采用χ2检验比较各组基因型的差异。    结果     饮用水中MCs的浓度连续4年超过国家规定标准,水产品中MCs阳性率为100%;GSTM1缺失频率在肝损伤组和对照组分别为43.2%和47.8%,两组间无显著统计学差异(P=1.00);GSTT1缺失频率在肝损伤组为60.5%,高于对照组39.1%,两组间有显著统计学差异(P=0.006);GSTM1和GSTT1共同缺失频率在肝损伤组为29.6%,同样高于对照组中16.3%,两组间有显著统计学差异(P=0.045)。    结论    GSTT1缺失可能是在MCs暴露人群肝损伤的易感因素;GSTM1和GSTT1共同缺失将会增加MCs暴露人群发生肝损伤的危险性。
Abstract:
Objective    To study the role of genetic polymorphisms of glutathione Stransferases (GSTT1, GSTM1) in persons with liver damage due to microcystin (MC) exposure.     Methods    Concentrations of MC in drinking water and aquatic products in Fuling District (Chongqing, China) were measured by enzyme-linked immunosorbent assay, and 81 persons with liver damage were randomly selected as an observation group and 92 healthy persons served as a control group. Genetic polymorphisms of GSTM1 and GSTT1 were assayed by multiplex PCR. Difference in genotypes was detected by Chi square test.     Results    The concentration of MC in drinking water was higher than the national standard in the past 4 consecutive years, and the positive MC rate was 100% for aquatic products. The frequency of GSTM1 null genotype was 43.2% in observation group and 47.8% in control group (P>0.05). The frequency of GSTT1 null genotype was 60.5% in observation group and 39.1% in control group (P<0.05). The percentage of GSTM1 and GSTT1 null genotypes was 29.6% in observation group and 39.1% in control group (P<0.05).     Conclusion     GSTT1 null genotype may be the susceptible factor for liver damage in persons exposed to MC. GSTM1 and GSTT1 null genotypes may increase the risk of developing liver damage in persons exposed to MC.

参考文献/References:

李砚, 陈济安, 赵清, 等. 谷胱甘肽转移酶基因多态性在微囊藻毒素致人群肝损伤发生中的作用[J].第三军医大学学报,2010,32(21):2322-2325.

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更新日期/Last Update: 2010-11-05