[1]叶平,杨波,唐文,等.p38MAPK信号通路对乙醛刺激的大鼠肝星状细胞增殖、凋亡及细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2197-2200.
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p38MAPK信号通路对乙醛刺激的大鼠肝星状细胞增殖、凋亡及细胞周期的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第20期
页码:
2197-2200
栏目:
论著
出版日期:
2010-10-30

文章信息/Info

Title:
p38MAPK signal pathway modulates proliferation, apoptosis and cell cycle of rat hepatic stellate cells stimulated by acetaldehyde
作者:
叶平杨波唐文卓强郑人源蒋明德
成都军区总医院消化内科;第三军医大学研究生管理大队;成都医学院门诊部
Author(s):
Ye Ping Yang Bo Tang Wen Zhuo Qiang Zheng Renyuan Jiang Mingde
Department of Gastroenterology,General Hospital of Chengdu Military Command, ChengDu, Sichuan Province, 610083; Department of Outpatients, Chengdu Medical College, Chengdu, Sichuan Province, 610083, China
关键词:
p38MAPK肝星状细胞乙醛细胞增殖细胞凋亡细胞周期
Keywords:
p38MAPK hepatic stellate cells acetaldehyde cell proliferation apoptosis cell cycle
分类号:
R322.47;R329.28;R363.13
文献标志码:
A
摘要:
目的   观察用特异性p38丝裂原活化蛋白激酶抑制剂SB203580阻断p38MAPK信号通路对乙醛刺激的大鼠肝星状细胞(hepatic stellate cell,HSC)增殖、凋亡及细胞周期的影响。   方法   体外培养大鼠HSC株,用SB203580(浓度为5、10、20 μmol /L)对乙醛(浓度200 μmol /L)刺激的大鼠HSC进行干预,分别以酶标仪及流式细胞仪检测HSC增殖、凋亡及周期的变化。   结果   不同浓度SB203580对HSC增殖有抑制作用,增殖抑制率分别为21.6%、27.0%、31.5%,与乙醛对照组有显著差异(P<0.05),且呈剂量效应关系;对HSC凋亡有促进作用,凋亡率分别为(13.7±0.3)%、(14.9±0.2)%、(16.1±0.2)%,凋亡率逐渐增加,与乙醛对照组有显著差异(P<0.05),且呈剂量效应关系;可以明显阻滞乙醛刺激的HSC由G1期进入S期,G1/G0期细胞比例逐渐增高[(29.1±1.9)%、(35.5±3.4)%、(46.0±2.6)%],S期细胞比例逐渐降低[(53.4±2.6)%、(48.4±3.6)%、(43.8±1.1)%],与乙醛对照组有显著差异(P<0.05),呈剂量效应关系。   结论   SB203580特异性阻断p38MAPK信号通路后,乙醛刺激的HSC增殖受到抑制,凋亡得到促进,细胞周期由G1期进入S期受到阻滞。
Abstract:
Objective  To investigate the effects of blocking p38MAPK by its specific blocker, SB203580, on the proliferation, apoptosis and cell cycle of rat hepatic stellate cells (HSCs).    Methods  Rat HSCs stimulated by acetaldehyde (200 μmol/L) were incubated with SB203580 at concentrations of 5, 10 and 20 μmol/L respectively. Cellular proliferation inhibition rate (CPIR) were analyzed by MTT assay, and the changes of apoptotic rate and cell cycle were analyzed by flow cytometry.    Results  Compared with acetaldehyde control group, SB203580 at different concentrations inhibited the proliferation of HSC significantly and the CPIR was 21.6%, 27.0% and 31.5% respectively (P<0.05). SB203580 also promoted the apoptosis of HSC significantly with the apoptotic rate of (13.7±0.3)%, (14.9±0.2)%, and (16.1±0.2)% respectively (P<0.05). And the cell percentage of G1/G0 was significantly increased by (29.1±1.9)%, (35.5±3.4)% and (46.0±2.6)% respectively (P<0.05), while, cell percentage of S was significantly decreased by (53.4±2.6)%, (48.4±3.6)% and (43.8±1.1)%(P<0.05).    Conclusion  After p38MAPK is blocked by SB203580 specifically in HSC stimulated by acetaldehyde, cell proliferation is inhibited but apoptosis promoted, and the cell cycle from G1 to S is arrested.

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更新日期/Last Update: 2010-10-19