[1]张子焕,许民辉,赖西南,等.爆炸致舱内大鼠高加速度负载时脑损伤的病理特点[J].第三军医大学学报,2010,32(19):2045-2048.
 Zhang Zihuan,Xu Minhui,Lai Xinan,et al.Pathological characteristics of brain injuries of rats exposed to blast high acceleration in airtight cabin[J].J Third Mil Med Univ,2010,32(19):2045-2048.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第19期
页码:
2045-2048
栏目:
论著
出版日期:
2010-10-15

文章信息/Info

Title:
Pathological characteristics of brain injuries of rats exposed to blast high acceleration in airtight cabin
作者:
张子焕许民辉赖西南王丽丽许明伟崔红
第三军医大学大坪医院野战外科研究所:神经外科,第六研究室,创伤、烧伤与复合伤国家重点实验室
Author(s):
Zhang Zihuan Xu Minhui Lai Xinan Wang Lili Xu Mingwei Cui Hong
State Key Laboratory of Trauma, Burns and Combined Injury, Department of Neurosurgery, Department 6, Institute of Surgery Research,  Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
密闭舱室加速度脑损伤病理特点
Keywords:
airtight cabin acceleration brain injury pathological characteristics
分类号:
R-322;R642;R651.15
文献标志码:
A
摘要:
目的 观察爆炸致舱内大鼠高加速度负载时脑损伤的病理特点,探讨其相关机制。 方法  引爆密闭舱室下方聚能盘内点爆源,模拟装甲车触雷爆炸,制作爆炸致舱内大鼠(坐姿)高加速度负载时脑损伤模型;48只SD雄性大鼠随机分为对照组、400 mg TNT爆炸当量致伤组(400 mg组)及800 mg TNT爆炸当量致伤组(800 mg组),每组16只,其中8只用于测量舱室内座位与大鼠头颅的加速度峰值(PA)及持续作用时间(TD),于伤后6 h处死,取脑、脊髓组织行病理检查,对海马CA1区正常锥体细胞记数及组织损伤分级,另8只行Morris水迷宫实验检测大鼠空间学习记忆功能。 结果  400 mg组PA座位为(5745±1036)g,TD座位为(6.87±0.58)ms,PA头颅为(701±309)g,TD头颅为(1.00±0.14)ms;800 mg组PA座位为(13 109±1167)g,TD座位为(11.08±1.43)ms,PA头颅为(3383±935)g,TD头颅为(1.32±0.18)ms,两组数据对应参数之间差异显著(P<0.01)。伤后6 h大鼠大体解剖无明显异常,但光镜下大脑、脊髓存在神经元急性损伤改变,800 mg组电镜下还见大脑毛细血管、轴索损伤。海马CA1组织学损伤分级:800 mg组>400 mg组>对照组;神经元密度:800 mg组<400 mg组<对照组(P<0.01)。与对照组比较,第1~4天 800 mg组逃避潜伏期延长(P<0.05),学习记忆曲线右移,延后1 d趋于稳定,经过平台次数及跨越目标象限时间减少(P<0.05);400 mg组介于两者之间。 结论  爆炸致舱内大鼠高加速度负载时大鼠可出现轻度弥漫性脑损伤,可能产生相应的早期症状或晚期后遗症,可推得装甲车辆触雷爆炸底板未击穿、变形小时乘员存在脑损伤可能。
Abstract:
Objective   To observe the pathological characteristics of brain injuries of rats exposed to blast high acceleration in airtight cabin and to study the relevant mechanism.  Methods   Columnar explosive in a metal energy concentration disk under airtight cabin was detonated, imitating armored vehicle mine blast, to establish a model of brain injuries induced by blast high acceleration in airtight cabin. Forty-eight rats were randomly divided into control group, 400 mg TNT explosion equivalent group (400 mg group) and 800 mg TNT explosion equivalent group (800 mg group), 16 rats in each group. Eight rats in each injury group were used to record the peak acceleration (PA) and the time of acceleration duration (TD) of the seat and skull as evaluation indexes of the acceleration load on the rats. Six hours after explosion, pathological changes of spinal cord and brain tissues in rats were examined under light microscope or transmission electron microscope (TEM). Meanwhile, the number of intact pyramidal cells per 1 mm linear length of CA1 was determined and neuronal damage to the hippocampal CA1 subfield was semi-quantitatively graded using a 0-3 rating system according to the method of Kato et al. The spatial learning and memory of another 8 rats were assessed with Morris water maze (MWM) after explosion.  Results   The PA and TD of seat were (5 745±1 036)g and (6.87±0.58)ms, (701±309) g and (1.00±0.14)ms of skull respectively in 400 mg group,(13 109±1167)g and(11.08±1.43)ms,(3 383±935)g  and (1.32±0.18)ms of skull respectively in 800 mg group (P<0.01). No visible change was observed in brain of injury groups, but acute neuronal damage to cerebral cortex, hippocampus and spinal cord was observed under microscope. Damage to capillary and axon was observed in 800 mg group under TEM. The damage score was the highest in 800 mg group and the lowest in control group. The neuronal density of hippocampal CA1 subfield was the lowest in 800 mg group and the highest in control group (P<0.01). During the first 4 d after explosion, the escape latency was significantly longer in 800 mg group than in control group (P<0.05). The learning and memory curve was shifted right in 800 mg group and became stable after 1 d. The frequency of crossing the target platform was lower and the time spent in crossing the target quadrant was longer in 800 mg group than in control group (P<0.05). The value of these 2 parameters was a median in 400 mg group.  Conclusion   Rats exposed to blast high acceleration in airtight cabin may suffer from mild diffused brain injury which may induce early clinical symptoms or late sequel. Occupants may suffer from brain injuries while armored vehicles can be attacked by landmines in the absence of a bottom penetration and large displacement, which should attract more attention of armored vehicle designers and clinicians.

参考文献/References:

张子焕, 许民辉, 赖西南, 等. 爆炸致舱内大鼠高加速度负载时脑损伤的病理特点[J].第三军医大学学报,2010,32(19):2045-2048.

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更新日期/Last Update: 2010-10-08