[1]王颖,万跃,谭兵,等.霉酚酸酯抑制转化生长因子-β在肺成纤维细胞中的表达及应用价值[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1413-1416.
 Wang Ying,Wan Yue,Tan Bing,et al.Inhibitory effect of mycophenolate mofetil on expression of TGF-β in pulmonary fibroblasts and its application value[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1413-1416.
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霉酚酸酯抑制转化生长因子-β在肺成纤维细胞中的表达及应用价值(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第13期
页码:
1413-1416
栏目:
论著
出版日期:
2010-07-15

文章信息/Info

Title:
Inhibitory effect of mycophenolate mofetil on expression of TGF-β in pulmonary fibroblasts and its application value
作者:
王颖万跃谭兵蒋勇白玉吴永忠
重庆市肿瘤研究所放疗科
Author(s):
Wang Ying Wan Yue Tan Bing Jiang Yong Bai Yu Wu Yongzhong
Department of Radiology, Chongqing Cancer Institute, Chongqing 400030,China
关键词:
MMF转化生长因子-β肺成纤维细胞
Keywords:
mycophenolate mofetilTGF-βWI26 cell
分类号:
R322.35;R394.2;R979.5
文献标志码:
A
摘要:
目的    探讨霉酚酸酯(mycophenolate mofetil,MMF)抑制转化生长因子-β(transforming growth factor-β,TGF-β)在肺成纤维细胞WI26中的表达和应用价值。    方法    肺成纤维细胞WI26常规细胞培养后分4组:对照组、MMF组、TGF-β组、MMF+TGF-β组。采用逆转录聚合酶链反应(RT-PCR) 检测TGF-β介导相关基因COL1A1的表达,Western blot和氯霉素乙酰基转移酶实验(chloramphenicol acetyl transferase,CAT)方法检测COL1蛋白的表达;胶原基质收缩实验和细胞伤痕实验观察胶原纤维的收缩和细胞移行能力的差异。    结果    MMF作用细胞24、48 h后,COL1A1 mRNA表达分别为对照组的(77.0±2.9)% 和(38.0±3.7)%,MMF降低了COL1A1 mRNA水平(P<0.05);TGF-β作用细胞24、48 h后,COL1A1 mRNA表达分别为对照组的(134.0±3.1)%和(189.0±2.4)%,TGF-β提高了COL1A1 mRNA水平(P<0.05);MMF+TGF-β作用细胞24,48 h 后,COL1A1 mRNA表达分别为对照组的(95.0±2.7)%和(71.0±3.3)%(P<0.05),48 h后MMF阻止了TGF-β诱导的COL1A1 mRNA表达。48 h后MMF组COL1蛋白表达为对照组的(44.0±1.9)%(P<0.05);TGF-β组COL1蛋白表达为对照组的(145.0±1.5)%(P<0.05);MMF+TGF-β组COL1蛋白表达为对照组的(79.0±2.5)%,MMF阻止TGF-β诱导的COL1蛋白表达。胶原基质收缩实验(第0、1、5天观察)和细胞伤痕实验(第0、8、24小时观察)结果显示:MMF重建成纤维细胞的接触抑制生长。    结论    MMF能阻止TGF-β介导的细胞纤维化。
Abstract:
Objective    To study the inhibitory effect of mycophenolate mofetil (MMF) on expression of TGF-β in pulmonary fibroblasts (WI26 cells) and its application value.     Methods    Pulmonary fibroblasts were divided into control group, MMF group, TGF-β group, and MMF+TGF-β group, after routine culture. Expression of TGF-β-induced COL1A1 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Expression of COL1 proteins was detected by Western blot analysis and chloramphenicol acetyl transferase assay, respectively. Difference in contractility of collagen fibers and migration of cells was observed in collagen matrix contraction test and cell scratch test.     Results    The expression level of COL1A1 mRNA was higher in MMF group than in control group 24 and 48 h after treatment with MMF (77.0%±2.9% vs 38.0%±3.7%), and was lower in MMF group than in control group 24 and 48 h after treatment with TGF-β and MMF+ TGF-β (134.0%±3.1% vs 189.0%±2.4%, and 95.0%±2.7% vs 71.0%±3.3%, P<0.05), indicating that MMF can inhibit the expression of COL1A1 mRNA 48 h after treatment with TGF-β.  The expression level of COL1A1 protein was 44.0%±1.9%, 145.0%±1.5%, and 79.0%±2.5% lower in MMF, TGF-β, and MMF+ TGF-β groups, respectively, than in control group, 48 h after treatment with TGF-β, indicating that MMF can suppress the expression of COL1A1 protein. Collagen matrix contraction test and cell scratch test showed that MMF can inhibit the growth of fibroblasts.     Conclusion    MMF can protect cells against TGF-β-induced fibrosis.

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更新日期/Last Update: 2010-07-09