[1]邓永兵,唐文渊.过氧化物酶体增殖物激活受体γ激动剂吡格列酮对大鼠创伤性脑损伤的影响[J].第三军医大学学报,2010,32(15):1638-1641.
 Deng Yongbing,Tang Wenyuang.Effect of pioglitazone on nerves of rats following traumatic brain injury[J].J Third Mil Med Univ,2010,32(15):1638-1641.
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过氧化物酶体增殖物激活受体γ激动剂吡格列酮对大鼠创伤性脑损伤的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第15期
页码:
1638-1641
栏目:
论著
出版日期:
2010-08-15

文章信息/Info

Title:
Effect of pioglitazone on nerves of rats following traumatic brain injury
作者:
邓永兵唐文渊
重庆医科大学附属第一医院神经外科;重庆市急救医疗中心神经外科
Author(s):
Deng YongbingTang Wenyuang
Department of Neurosurgery, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016; Department of Neurosurgery, Chongqing First Aid Medical Center, Chongqing, 400014, China
关键词:
PPARγ吡格列酮脑损伤细胞凋亡神经保护
Keywords:
peroxisome proliferator-activated receptor gamma pioglitazone brain trauma apoptosis neural protection
分类号:
R-332;R651.15;R971
文献标志码:
A
摘要:
目的    观察PPARγ激动剂吡格列酮对大鼠创伤性脑损伤的神经保护作用。    方法    将72只SD大鼠按随机数字表法分为假致伤组、对照组、吡格列酮治疗组,每组24只。采用改良的Feeney法制作脑创伤模型,治疗组采用吡格列酮(10 mg/kg)灌胃,假致伤组和对照组用等量生理盐水灌胃。致伤后在相应时相点行大鼠神经功能评分后,用干湿质量法进行脑组织含水量测定,进行HE、Nissl及TUNEL染色观察脑组织损伤、迟发性神经元死亡及神经细胞凋亡程度。    结果    ①在伤后48 h、5 d,治疗组的神经功能评分[分别为(2.12±0.58)、(1.67±0.78)]好于对照组[(2.67±0.65)、(2.25±0.62),P<0.05];②伤后24 h治疗组与对照组脑组织含水量差异无统计学意义[分别为(78.84±1.92)%、(79.21±2.20)%,P>0.05];③伤后48 h,治疗组迟发性神经元死亡(38.59±1.97)%和神经细胞凋亡数(31.67±4.76)明显低于对照组[分别为(51.25±4.01)%、(45.33±4.68),P<0.05]。    结论    PPARγ激动剂吡格列酮能抑制创伤性脑损伤后的神经细胞凋亡,保护神经元,从而发挥神经保护作用。
Abstract:
Objective    To study the neural protective effect of pioglitazone, a peroxisome proliferator-activated receptor gamma (PPARγ) agonist, on nerves of rats following traumatic brain injury (TBI).     Methods    Seventy-two male Sprague-Dawley rats were randomly divided into pioglitazone treatment group, sham operation group, and control group (n=24 rats in each group). Rats in pioglitazone treatment received pioglitazone gastric perfusion (10 mg/kg) while rats in sham operation group underwent normal saline gastric perfusion (10 mg/kg). A rat model of brain injury was established with the Feeney’s free falling method. After neural function of rats was scored, water content in brain tissue was measured with the dry-wet weighing method. Injury of brain tissue, delayed death of neurons and apoptosis of nerve cells were observed with HE, TUNEL and Nissl’s staining, respectively.     Results    The neural function score was higher in pioglitazone treatment group than in control group in 48 h and 5 d after TBI (P<0.05). No significant difference was found in brain tissue water content between pioglitazone treatment and control groups in 48 h after TBI [(78.84±1.92)% vs (79.21±2.20)%, P>0.05]. The number of delayed dead and apoptotic nerve cells was significantly lesser in pioglitazone treatment group than in control group in 48 h after TBI (P<0.05).     Conclusion    Pioglitazone can effectively inhibit the apoptosis of nerve cells, thus playing a role in protecting nerve cells of rats following TBI.

参考文献/References:

邓永兵,唐文渊. 过氧化物酶体增殖物激活受体γ激动剂吡格列酮对大鼠创伤性脑损伤的影响[J].第三军医大学学报,2010,32(15):1638-1641.

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更新日期/Last Update: 2010-08-03