[1]王渝琦,徐光旭,耿卫朴,等.人子宫颈癌PD-L1的表达及其与肿瘤内浸润T细胞的相关性研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1173-1176.
　Wang Yuqi,Xu Guangxu,Geng Weipu,et al.Expression of programmed death receptor-ligand 1 in human cervical carcinoma and its correlation with tumor infiltrating T cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1173-1176.
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人子宫颈癌PD-L1的表达及其与肿瘤内浸润T细胞的相关性研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第11期
页码:
1173-1176
栏目:
论著
出版日期:
2010-06-15

文章信息/Info

Title:
Expression of programmed death receptor-ligand 1 in human cervical carcinoma and its correlation with tumor infiltrating T cells
作者:
王渝琦; 徐光旭; 耿卫朴; 唐均英; 徐曼
重庆医科大学:基础医学院病理学教研室,分子医学与肿瘤研究中心,附属第一医院妇产科
Author(s):
Wang Yuqi;  Xu Guangxu;  Geng Weipu;  Tang Junying;  Xu Man
Department of Pathology，Molecular Medicine and Tumor Research Center，Department of Gynecology and Obstetrics, First Affiliated Hospital，Chongqing Medical University，Chongqing, 400016, China
关键词:
子宫颈癌; PD-L1; CD4+ T cell; CD8+ T cell; 凋亡
Keywords:
cervical carcinoma; programmed death receptor-ligand 1; CD4+T cell; CD8+T cell; apoptosis
分类号:
R737.33;R392.11;R392.3
文献标志码:
A
摘要:
目的    观察负性免疫调节分子程序性死亡配体1（programmed death receptor-ligand 1，PD-L1）在人子宫颈癌的表达及其与肿瘤内T淋巴细胞浸润的关系。    方法    免疫组织化学染色观察5例正常宫颈组织、7例高级别宫颈上皮内瘤变（cervical intraepithelial neoplasia，CINⅡ-Ⅲ）组织和67例子宫颈癌组织中PD-L1的表达和淋巴细胞PD-1的表达，免疫荧光染色观察相应组织内浸润的CD4+T和CD8+T细胞数量，TUNEL法检测淋巴细胞凋亡。    结果    正常子宫颈上皮不表达PD-L1；宫颈瘤变上皮不表达或弱阳性表达PD-L1，70%（47/67）的宫颈癌表达PD-L1。肿瘤内浸润的部分淋巴细胞表达PD-1。子宫颈瘤变上皮与子宫颈鳞状细胞癌PD-L1表达存在明显差异（P<0.01），浅表浸润的子宫颈鳞状细胞癌PD-L1 的表达略低于深部浸润的鳞状细胞癌，但无显著差异；PD-L1阳性病例肿瘤局部CD8+T细胞数量显著减少，且PD-L1 表达与CD8+T细胞数量明显负相关（r=-0.82，P<0.01），但与肿瘤局部CD4+T细胞数量无明显相关性（r=-0.05，P>0.05）；部分PD-L1阳性的子宫颈癌局部浸润的淋巴细胞存在凋亡现象。    结论    子宫颈癌细胞表达PD-L1，且PD-L1的表达与肿瘤局部浸润的CD8+T细胞数量减少有关，但与浸润的CD4+T细胞数量无关。癌细胞表达PD-L1可能促进了肿瘤内浸润的淋巴细胞凋亡。
Abstract:
Objective    To observe the expression of programmed death receptor-ligand 1 (PD-L1) in human cervical carcinoma and its correlation with tumor T cell infiltration.     Methods    Expression of PD-L1 and PD-1 was detected in 5 normal cervical tissue samples，7 high-level cervical intraepithelial neoplasia (CIN Ⅱ-Ⅲ) tissue samples and 67 cervical carcinoma tissue samples, respectively, with immunohistochemistry staining. The number of tumor infiltrating CD4+T and CD8+T cells was counted with immunofluorescent staining. Apoptotic tumor infiltration lymphocytes were examined by TUNEL assay.     Results    PD-L1 was not expressed in normal cervical epithelium and negatively or weakly expressed in cancerous cervical epithelium. PD-L1 was expressed in 70% (47/67) of cervical carcinoma tissue samples.  PD-1 was expressed in partial tumor infiltrating lymphocytes (P<0.01). The expression level of PD-L1 was slightly lower in superficial invasive cervical carcinoma tissue samples than in deep invasive carcinoma tissue samples. The number of CD8+T cells was significantly decreased in patients with positive PD-L1.The expression of PD-L1 was negatively correlated with the number of tumor infiltrating CD8+T cells (r=-0.82，P<0.01) but not with the number of CD4+T cells (r=-0.05，P> 0.05). Apoptosis occurred in partial tumor infiltrating lymphocytes of cervical carcinoma tissue samples.     Conclusion    PD-L1 is expressed in cervical carcinoma cells. The expression of PD-L1 is correlated with the reduced number of tumor infiltrating CD8+T cells but not with the number of CD4+T cells. PD-L1 expression in tumor cells may play role in apoptosis of tumor infiltrating lymphocytes.

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更新日期/Last Update: 2010-06-04