[1]沈婕,夏新新,邹俊杰,等.通络方剂对糖尿病大鼠肝纤维化相关血清学指标及形态学的影响[J].第三军医大学学报,2010,32(12):1296-1300.
 Shen Jie,Xia Xinxin,Zou Junjie,et al.Effects of Tongluo recipe on serum hepatic fibrosis markers and hepatic morphological changes in diabetic rats[J].J Third Mil Med Univ,2010,32(12):1296-1300.
点击复制

通络方剂对糖尿病大鼠肝纤维化相关血清学指标及形态学的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第12期
页码:
1296-1300
栏目:
论著
出版日期:
2010-06-30

文章信息/Info

Title:
Effects of Tongluo recipe on serum hepatic fibrosis markers and hepatic morphological changes in diabetic rats
作者:
沈婕夏新新邹俊杰石勇铨刘志民
第二军医大学长征医院内分泌科
Author(s):
Shen Jie Xia Xinxin Zou Junjie Shi Yongquan Liu Zhimin
Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China
关键词:
糖尿病肝纤维化通络方剂氧化性应激血清学试验组织学大鼠
Keywords:
diabetes mellitus liver cirrhosis tongluo recipe oxidative stress serologic tests histology rats
分类号:
R289.5;R587.2;R575.02
文献标志码:
A
摘要:
目的    探讨通络方剂(Tongluo recipe,TLR)对糖尿病大鼠肝纤维化相关血清学指标及形态学的影响。    方法    SD大鼠予链脲佐菌素(streptozotocin, STZ)60 mg/kg腹腔注射,制备糖尿病模型。模型动物按随机数字表法分为2组,每组10只:糖尿病对照组、通络方剂干预组[TLR溶于生理盐水,剂量为2.0 g/(kg·d)],同时取10只大鼠作为正常对照组,正常对照组和糖尿病对照组大鼠仅给予等量的生理盐水溶液,每日同一时间灌胃。造模12周后测血清肝纤维化指标,取肝组织测超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA),观察肝组织形态学改变。    结果    糖尿病大鼠血清透明质酸(hyaluronic acid ,HA)、Ⅳ型胶原(collagen type Ⅳ,Ⅳ-c)水平显著升高(P<0.01),肝血窦边缘及肝汇管区胶原纤维增生,Disse间隙贮脂细胞增生;糖尿病大鼠肝组织SOD活性显著降低,MDA水平显著升高(P<0.01)。经通络方剂治疗12周后,血清HA、Ⅳ-c水平显著降低(P<0.05),肝纤维化病理形态有明显改善;糖尿病大鼠肝组织SOD活性显著增加、MDA水平显著下降(P<0.01),氧化应激水平有所下降。    结论    通络方剂可降低糖尿病大鼠血清肝纤维化指标,改善肝纤维化的病理形态,其作用机制可能与其增强了肝组织抗氧化酶类活性以及清除脂质过氧化物有关。
Abstract:
Objective    To investigate the effects of Tongluo recipe (TLR), a compound ripe of traditional Chinese drugs, on serum hepatic fibrosis markers and hepatic morphological changes of diabetic rats.      Methods    Experimental diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg) in male Sprague-Dawley rats. The rats were divided into normal control (CN), diabetes mellitus control (DM), and TLR treatment group [TRL, 2.0 g/(kg·d), once a day, intragastrical gavage], 10 rats in each group. After 12 weeks of treatment, serum hepatic fibrosis markers hyaluronic acid (HA), collagen type Ⅳ (Ⅳ-c), laminin (LN), procollagen type Ⅲ (PC Ⅲ), and superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissues were measured. The morphological changes of liver tissues were observed with light microscopy and transmission electronic microscopy.      Results    The serum HA and Ⅳ-c levels of diabetic rats were increased (P<0.01) compared with CN group. Light microscopy and transmission electron microscopy displayed that there were collagen fibers hyperplasia in hepatic sinusoid and portal area, and fat-storing cells hyperplasia in Disse space. SOD activities were decreased and the MDA levels were increased in liver tissues of diabetic rats than in those of CN rats (P<0.01). After 12 weeks’ TLR treatment, the serum HA and Ⅳ-c levels were decreased than those in DM group (P<0.05) and morphological changes were ameliorated. TLR treatment obviously improved SOD activity and decreased the levels of MDA (P<0.01).      Conclusion    Tongluo recipe decreases the levels of serum hepatic fibrosis markers and ameliorates morphological changes of hepatic fibrosis in diabetic rats, which probably through increasing activities of antioxidatant and depleting lipid peroxidation products. 

参考文献/References:

沈婕,夏新新,邹俊杰,等.通络方剂对糖尿病大鼠肝纤维化相关血清学指标及形态学的影响[J].第三军医大学学报,2010,32(12):1296-1300.

相似文献/References:

[1]牟海刚,古平.莱姆病伴直立性低血压误诊为糖尿病心血管自主神经病1例[J].第三军医大学学报,2012,34(15):1542.
[2]刘波,吴小翎,张霞,等.福辛普利对非酒精性脂肪性肝炎大鼠肝纤维化的作用[J].第三军医大学学报,2012,34(16):1646.
 Liu Bo,Wu Xiaoling,Zhang Xia,et al.Effects and mechanism of fosinopril on hepatic fibrosis in rats with nonalcoholic steatohepatitis[J].J Third Mil Med Univ,2012,34(12):1646.
[3]张立婷,赵荣荣,陈红,等.HBV-DNA低复制慢性乙肝患者血清HA、LN、PcⅢ、CⅣ水平与肝纤维化病理分期相关性分析[J].第三军医大学学报,2012,34(17):1783.
 Zhang Liting,Zhao Rongrong,Chen Hong,et al.Correlation between serum levels of hyaluronic acid, laminin, procollagen Ⅲ and collagen Ⅳ and pathological stage of hepatic fibrosis in chronic hepatitis B patients with low duplication of HBV-DNA[J].J Third Mil Med Univ,2012,34(12):1783.
[4]周厚地,童强,段炼,等.胃大部切除术致糖尿病患者反复低血糖1例[J].第三军医大学学报,2012,34(17):1753.
[5]吴艳,王毅,曾绍凡,等.老年2型糖尿病瘦素、骨密度变化及相关因素的研究[J].第三军医大学学报,2007,29(22):2188.
 WU Yan,WANG Yi,ZENG Shao-fan,et al.Bone density, leptin level and other related factors in elder patients with type 2 diabetes[J].J Third Mil Med Univ,2007,29(12):2188.
[6]李志勤.饮食护理干预对老年糖尿病患者血糖和糖化血红蛋白水平的影响[J].第三军医大学学报,2012,34(20):2130.
[7]徐灵莉,邓本敏,唐玲,等.护理风险管理能有效提高糖尿病合并恶性肿瘤患者的化疗疗效[J].第三军医大学学报,2013,35(04):351.
 Xu Lingli,Deng Benmin,Tang Ling,et al.Risk management of nursing improves chemotherapy efficiency for diabetic patients with malignant tumor[J].J Third Mil Med Univ,2013,35(12):351.
[8]魏平.糖尿病治疗的新趋势及相关热点追踪[J].第三军医大学学报,2012,34(23):2435.
[9]李明秀,王建,樊荣,等.癌症合并糖尿病患者住院期间的营养治疗[J].第三军医大学学报,2007,29(21):2079.
[10]黄月红,陈运新,郑伟达,等.肝脏靶向表达大鼠白介素-10基因对猪血清诱导的大鼠肝纤维化的抑制[J].第三军医大学学报,2011,33(13):1353.
 Huang Yuehong,Chen Yunxin,Zheng Weida,et al.Targeting expression of rat interleukin-10 in liver suppresses pig serum-induced hepatic fibrosis in rats[J].J Third Mil Med Univ,2011,33(12):1353.

更新日期/Last Update: 2010-06-22