[1]李新,李敏,涂柳,等.颈交感神经阻滞对重度烧伤大鼠肝脏的保护机制初探[J].第三军医大学学报,2010,32(17):1812-1215.
 Li Xin,Li Min,Tu Liu,et al.Protective effect of cervical sympathetic block on severely burned rat liver and its related mechanism[J].J Third Mil Med Univ,2010,32(17):1812-1215.
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颈交感神经阻滞对重度烧伤大鼠肝脏的保护机制初探(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第17期
页码:
1812-1215
栏目:
论著
出版日期:
2010-09-15

文章信息/Info

Title:
Protective effect of cervical sympathetic block on severely burned rat liver and its related mechanism
作者:
李新李敏涂柳艾国平王军平王峰超粟永萍
第三军医大学:西南医院全军泌尿外科研究所,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室;重庆医科大学基础医学院生理学教研室
Author(s):
Li XinLi MinTu LiuAi GuopingWang JunpingWang FengchaoSu Yongping
Department of Urology, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038; Department of Physiology, Chongqing Medical University, Chongqing, 400016, China
关键词:
交感神经阻滞烧伤肝脏保护机制
Keywords:
cervical sympathetic block burn injury liver protection
分类号:
R322.47; R644; R363
文献标志码:
A
摘要:
目的    观察颈交感神经阻滞(cervical sympathetic block ,CSB)对严重烧伤大鼠肝脏功能与结构的保护作用,并对其可能保护机制进行探讨。    方法      将动物按随机数目表随机分为对照组、严重烧伤组和CSB治疗组,制作重度烧伤模型(TBSA 20% Ⅲ度),CSB治疗组伤后以0.1%罗哌卡因行颈交感神经双侧阻滞。通过检测肝功能、肝组织抗氧化能力、Bcl-2表达以及肝组织病理改变判断CSB对肝脏的保护作用。通过检测监测血压变化及检测肝脏、下丘脑血流灌注量来推断其可能机制。    结果      CSB组大鼠伤后血浆白蛋白下降及谷丙转氨酶上升不明显,1、3、5、7 d时相点血浆白蛋白含量分别为(48.98±6.89)、(38.61±4.35)、(24.05±3.62)和(22.57±4.10) mmol/L,血浆ALT浓度分别为(48.15±7.37)、(58.21±5.43)、(62.07±8.16)、(56.87±5.88)IU/L,较烧伤组为好(P<0.01);CSB组肝组织总抗氧化能力分别为(3.16±0.17)、(2.88±0.15)、(2.71±0.16)、(2.61±0.58) U/mg,较烧伤组明显增强(P<0.01);CSB组大鼠伤后血压较为稳定,伤后1、3、5、7 d时相点分别为(90.58±5.69)、(82.69±3.81)、(70.61±9.51)、(82.05±2.36 )mmHg,与烧伤组相比差异显著(P<0.01);CSB组动物伤后脏器血流灌注较好,伤后即刻、1、3、5 d时相点肝血流量分别为(0.302±0041)、(0.284±0.024)、(0.16±0.023)、(0.256±0.03) ml/(min·g),下丘脑血流量分别为(1.013±0.113)、(0.721±0.110)、(0.944±0.438)、(0.765±0.316) ml/(min·g),均较烧伤组有明显升高(P<0.01);肝组织Bcl-2的表达明显增高;CSB组大鼠伤后肝组织病理改变较轻,主要表现为肝细胞浊肿,嗜酸性变,肝细胞脂肪变性。    结论      颈交感神经阻滞对重度烧伤大鼠肝脏具有保护作用,这种保护作用可能是通过调节下丘脑HPA轴和交感-肾上腺轴来进行的。
Abstract:
Objective  To study the protective effect of cervical sympathetic block (CSB) on liver function and structure of severely burned rats and its possible mechanism.   Methods  A rat model of severe burn (TBSA 20% Ⅲ) was induced by blocking bilateral cervical sympathetic nerves with 0.01% Ropivacaine. The rats were divided into control group, burn group and CSB group. Protective effect of CSB on liver function, antioxidation, Bcl-2 expression and pathologic change in liver tissue was detected. Its mechanism was studied by measuring blood pressure and blood perfusion volume in liver and hypothalamus.    Results  The levels of plasma-albumin in CSB group were 48.98±6.89, 38.61±4.35, 24.05±3.62 and 22.57±4.10 mmol/L respectively 1, 3, 5 and 7 d after burn.  The levels of ALT were higher in CSB group (48.15±7.37, 58.21±5.43, 62.07±8.16 and 56.87±5.88 IU/L respectively 1, 3, 5 and 7 d after burn)than in burn group,(P<0.01). The hepatic T-AOC values were higher in CSB group (3.16±0.17, 2.88±0.15, 2.71±0.16, and 2.61±0.58 respectively 1, 3, 5 and 7d after burn) than in burn group(P<0.01). The blood pressures were more stable in CSB group (90.58±5.69, 82.69±3.81, 70.61±9.51 and 82.05±2.36 mmHg respectively 1, 3, 5 and 7 d after burn) than in burn group (P<0.01). The hepatic and hypothalamic blood flow volumes were higher in CSB group [0.302±0.041, 0.284±0.024, 0.16±0.023, 0.256±0.03 ml/(min·g) and 1.013±0.113, 0.721±0.110, 0.944±0.438, 0.765±0.316 ml/(min·g) respectively burned instantly, 1, 3 and 5 d after burn] than in burn group(P<0.01).The pathologic changes of liver structure were milder while the expression level of Bcl-2 in liver was higher in CSB group than in burn group.   Conclusion  CSB can protect the liver of severely burned rats by regulating the hypothamus axis and sympathetic-kidney axis.

参考文献/References:

李新, 李敏,  涂柳, 等. 颈交感神经阻滞对重度烧伤大鼠肝脏的保护机制初探[J].第三军医大学学报,2010,32(17):1812-1815.

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更新日期/Last Update: 2010-09-08