[1]杨美,孙善全,余维华,等.低渗状态下星形胶质细胞AQP8的表达变化[J].第三军医大学学报,2010,32(11):1136-1140.
 Yang Mei,Sun Shanquan,Yu Weihua,et al.Expression of aquporin 8 in rat astrocytes under hypotonic stress[J].J Third Mil Med Univ,2010,32(11):1136-1140.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第11期
页码:
1136-1140
栏目:
论著
出版日期:
2010-06-15

文章信息/Info

Title:
Expression of aquporin 8 in rat astrocytes under hypotonic stress
作者:
杨美孙善全余维华刘辉卓飞邱国平
重庆医科大学:基础医学院人体解剖学教研室,神经科学研究中心
Author(s):
Yang Mei Sun Shanquan Yu Weihua Liu Hui Zhuo Fei Qiu Guoping
Department of Human Anatomy, College of Basic Medical Sciences, Chongqing Medical University, Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China
关键词:
水通道蛋白8星形胶质细胞渗透压
Keywords:
aquaporin8 astrocyte osmotic stress
分类号:
R322.81;R329.28;R338.1
文献标志码:
A
摘要:
目的    探讨低渗透压作用下,星形胶质细胞水通道蛋白8(aquaporin8,AQP8)的表达变化规律。    方法    原代培养大鼠星形胶质细胞,随机分为对照组(常规培养液)和低渗组(268、254、240 mmol/L),每组分别作用1、3、6、12、24 h,共5个时间点。通过MTT比色法检测细胞活力,免疫细胞化学、免疫荧光、Real time-PCR研究AQP8及其mRNA的表达变化。    结果    对照组星形胶质细胞的细胞形态、细胞活性、AQP8及其mRNA的表达在各时间点均无明显变化(P>0.05)。低渗组中,星形胶质细胞水肿,细胞活性下降;AQP8及其mRNA的表达水平随低渗程度的下调和低渗作用时间的延长而增强。低渗液(268、254、240 mmol/L)作用后,免疫细胞化学显示星形胶质细胞的AQP8表达从低渗作用1 h的(0.258 3±0.009 8, 0.280 0±0.010 9, 0.281 7±0.007 5) 到低渗作用24 h的(0.385 0±0.010 9, 0.406 7±0.007 0, 0.425 0±0.010 4) (P<0.05);Real time PCR显示:AQP8与beta-actin 的mRNA水平比从低渗作用1 h的(0.127 5±0.011 8,0.134 4±0.032 4, 0.167 5±0.007 8)上升到(3.278 0±0.121 2,5.410  4±0.104 1,8.351 0±0.288 0)。    结论    低渗作用下,AQP8介导的水分子运输可导致星形胶质细胞肿胀,继而引发细胞结构、功能的损伤; AQP8的表达上调可能是导致星形胶质细胞水肿形成和发展的重要分子机制之一。
Abstract:
Objective    To study the expression of aquaporin8 (AQP8) in rat astrocytes under hypotonic stress.     Methods    Primarily cultured rat astrocytes, randomly divided into control group (280 to 320 mmol/L) and 3 hypotonic groups at the concentration of 268, 254, and 240 mmol/L respectively, were exposed to hypotonic stress for 1,3,6,12,and 24 h, respectively. Viability of astrocytes was detected by MTT colorimetry. Expression of AQP8 at protein and  mRNA levels in astrocytes was detected by immunocytochemistry, immunofluorescence, and real time reverse transcription polymerase chain reaction (RT-PCR), respectively.     Results    No significant change was observed in morphology and viability of astrocytes as well as in expression of AQP8 and its mRNA at different time points in control group. Edema of astrocytes was observed, the viability of astrocytes was decreased, and the expression of AQP8 at protein and mRNA levels was in a negative concentration-dependent and positive time-dependent manner. Immunocytochemistry showed that the expression of AQP8 in astrocytes was 0.258 3±0.009 8, 0.280 0±0.010 9, 0.281 7±0.007 5 respectively after astrocytes were exposed to hypotonic stress at different concentrations for 1 h and were 0.385  0± 0.010 9, 0.406 7±0.007 0, 0.425 0±0.010 4, respectively, for 24 h  (P<0.05). RT-PCR showed that the expression of AQP8 in astrocytes was increased from 0.258 3±0.009 8, 0.280 0±0.010 9, 0.281 7±0.007 5 in 1 h after astrocytes were exposed to hypotonic stress at different concentrations to 0.385±0.010 9, 0.406 7±0.007 0, 0.425 0±0.010 4 in 24 h after exposure (P<0.05)compared to the expression of betaactin mRNA, which was increased from 0.127 5±0.011 8,0.134 4±0.032 4, 0.167 5±0.007 8 in 1 h after exposure to 3.278 0±0.121 2, 5.410 4±0.104 1, 8.351 0±0.288 0 respectively in 24 h after exposure (P<0.05).     Conclusion    AQP8-mediated water molecule transport can lead to edema, structure and function injury of astrocytes under hypotonic stress. Increased AQP8 expression may be one of the important mechanisms underlying  the formation and development of astrocyte edema. 

参考文献/References:

杨美,孙善全,余维华,等.低渗状态下星形胶质细胞AQP8的表达变化[J].第三军医大学学报,2010,32(11):1136-1140.

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更新日期/Last Update: 2010-06-04