[1]李招权,万莹铧,李军,等.外源性CCN1促进辐射损伤L929细胞的生长和迁移[J].第三军医大学学报,2010,32(11):1127-1130.
 Li Zhaoquan,Wan Yinghua,Li Jun,et al.Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells[J].J Third Mil Med Univ,2010,32(11):1127-1130.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第11期
页码:
1127-1130
栏目:
论著
出版日期:
2010-06-15

文章信息/Info

Title:
Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells
作者:
李招权万莹铧李军司维柯粟永萍杜业军潘静
第三军医大学:医学检验系临床血液学教研室,军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
Li Zhaoquan  Wan Yinghua  Li Jun Si Weike  Su Yongping Du Yejun Pan Jing
Department of Clinical Hematology, College of Laboratory Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Department of  Antiradiation Medicine, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China
关键词:
CCN1辐射损伤L929增殖迁移
Keywords:
CCN1 radiation injury L929 proliferation migration
分类号:
R818.05;R329.28;R394.2
文献标志码:
A
摘要:
目的    观察外源性CCN1是否促进辐射损伤L929细胞生长和迁移,以探讨CCN1在放创复合伤修复中的作用。     方法    以4 Gy γ射线辐射L929细胞作为辐射损伤细胞模型,细胞辐射后分别加入2 ml CCN1或RFP条件培养液,37℃,5%CO2培养,作为CCN1组和RFP组;空白条件培养液培养辐射损伤L929细胞作为空白组。各组进行MTT试验、平板克隆形成试验、细胞周期分析和划痕愈合试验。     结果    与RFP组和空白组相比,CCN1条件培养液处理后,与空白组辐射损伤L929细胞增殖明显增强(P<0.05),与RFP组和空白组相比,克隆形成率明显增高[CCN1组(34.4±3.6)%, RFP组(24.5±2.9)%,空白组(29.5±3.5)%](P<0.05),培养5 d时CCN1组S期细胞[(37.3±2.3)%]比RFP组[(25.2±1.9)%]和空白组[(26.9±1.3)%]增多(P<0.01),细胞迁移能力明显增强划痕愈合试验在1~5 d的愈合宽度/原宽度值与RFP组和空白组相比差异显著(P<0.01)。    结论    外源性CCN1可促进辐射损伤的成纤维细胞生长和迁移,提示CCN1是放创复合伤修复的促进因素之一。
Abstract:
Objective    To study the role of   Cysteine-rich 61 (Cyr61/CNN1) in repair of combined injury by observing whether exogenous CCN1 promotes the proliferation and migration of radiation-injured L929 cells.     Methods    A radiation model of L929 cells was induced by γ ray at a dose of 4 Gy. The irradiated L929 cells were cultured in a medium containing 2 ml adenovirus plasmids of CCN1 or RFP at 37 ℃ in an atmosphere containing 5%CO2, which served as a CCN1 group and a RFP group, respectively. Irradiated L929 cells cultured in a blank control medium served as a blank control group. Effects of CCN1 on proliferation and migration of radiationinjured L929 cells were detected by MTT assay, plate colony formation assay, cell cycle analysis, and scratch test of wound healing.     Results    The proliferation and colony formation rates of L929 cells cultured in a medium containing CCN1 were significantly higher than in RFP and control groups [colony formation rate: (34.4±3.6)% vs (24.5±2.9)% and (29.5±3.5)%, P<0.05]. Five hours after culture, the amount of L929 cells in the S phase was larger in CCN1 group than in RFP and control groups (37.3%±2.3% vs 252%±1.9% and 26.9%±1.3%, P<0.01). Scratch test of wound healing showed that the width/thickness ratio of migrated L929 cells in healed wounds was significantly higher in CCN1 group than in RFP and control groups (P<0.01).     Conclusion    Exogenous CCN1 promotes the proliferation and migration of radiation-injured L929 cells, suggesting that CCN1 is one of the stimulating factors for the repair of combined injury.

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更新日期/Last Update: 2010-06-04