[1]曾柱,龙金华.肝癌细胞对树突状细胞白介素-12表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1305-1307.
 Zeng Zhu,Long Jinhua.Hepatocellular carcinoma cell line Bel7402 suppresses IL-12 secretion of dendritic cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1305-1307.
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肝癌细胞对树突状细胞白介素-12表达的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第12期
页码:
1305-1307
栏目:
论著
出版日期:
2010-06-30

文章信息/Info

Title:
Hepatocellular carcinoma cell line Bel7402 suppresses IL-12 secretion of dendritic cells
作者:
曾柱龙金华
贵阳医学院基础医学院生物技术教研室;北京大学基础医学院生物物理系血液流变学研究中心;贵州省肿瘤医院头颈肿瘤科
Author(s):
Zeng Zhu Long Jinhua
Department of Biotechnology, School of Basic Medical Sciences, Guiyang Medical College, Guiyang, Guizhou Province, 550004; Hemorheological Research Center, Faculty of Biophysics, School of Basic Medical Sciences, Peking University,Beijing, 100083; Department of Head and Neck Tumor, Tumor Hospital of Guizhou, Guiyang, Guizhou Province, 550004, China
关键词:
树突细胞肝细胞白细胞介素12转化生长因子β1血管内皮生长因子
Keywords:
dendritic cellscarcinomahepatocellularinterleukin-12transforming growth factor beta1vascular endothelial growth factors
分类号:
R392.11;R730.23;R735.7
文献标志码:
A
摘要:
目的    研究肝癌细胞(hepatocellular carcinoma cells,HCCs)微环境对树突状细胞(dendritic cells, DCs)的白介素-12(interleukin-12, IL-12)表达的影响,以进一步理解肿瘤的免疫逃逸机制。    方法    免疫磁珠法从人外周血分离CD14+单核细胞,加入rhGM-CSF和rhIL-4将单核细胞诱导分化为imDCs,利用rhTNF-α将imDCs诱导为mDCs,imDCs和mDCs与HCCs在Transwell中共培养48 h,ELISA法分别检测不同培养条件下上清液中IL-10、IL-12、转化生长因子β1(trasnformed growth factor-β1,TGF-β1)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达量。    结果    与HCCs共培养后,imDCs和mDCs的IL-12的表达量分别从(115.076±8.129)pg/ml和(258.346±3.609)pg/ml下调至(17.599±1.757)pg/ml和(6.787±1.123)pg/ml (P<0.05, P<0.01),而且培养上清中的TGF-β1浓度分别从(133.172±3.053)pg/ml和(174.446±3.972)pg/ml上调至(633.025±9.527)pg/ml和(486.806±10.515)pg/ml(P<0.05,P<0.01),VEGF的浓度分别从0 pg/ml上调至(830.892±7.564)pg/ml和(858.169±5.419)pg/ml(P<0.01)。    结论    HCCs可抑制DCs的IL-12的表达,这可能是HCCs损伤DCs免疫调节功能的方式之一。
Abstract:
Objective    To study the influence of microenvironment created by dissoluble cytokines derived from hepatocellular carcinoma cells (HCCs) on the expression of IL-12 of dendritic cells (DCs) at different differentiating stage.     Methods    The CD14+ monocytes, isolated with immune magnetic beads from fresh peripheral blood of healthy human, were cultured in RPMI 1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 d to develop into immature DCs (imDCs). Maturation was induced by addition of 20 ng/ml rhTNF-α to imDCs for another 3 days’ culture. Acquired imDCs and mDCs were respectively co-cultured with human HCC Bel7402 cells for 48 h in Transwell chamber. The concentrations of IL-10, IL-12, TGF-β1 and VEGF in culture supernatant were investigated by ELISA, imDCs and mDCs cultured alone served as control.     Results    After co-cultured with Bel7402 cells, IL-12 were decreased from (115.076±8.129) pg/ml to (17.599±1.757) pg/ml in imDCs, and from (258.346±3.609) pg/ml to (6.787±1.123) pg/ml in mDCs (P<0.05, P<0.01). However, TGF-β1 concentrations in culture supernatant were increased from (133.172±3.053) pg/ml to (633.025±9.527) pg/ml in imDCs, and from (174.446±3.972) pg/ml to (486.806±10.515) pg/ml in mDCs (P<0.05, P<0.01). VEGF concentrations was from 0 pg/ml to (830.892±7.564) pg/ml and (858.169±5.419) pg/ml in imDCs and mDCs respectively (P<0.01).     Conclusion    HCCs inhibits secretion of IL-12 from DCs, which may be one of the ways which HCCs impair the immune regulatory function of DCs. 

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更新日期/Last Update: 2010-06-22