[1]郑必祥,彭代智,左海斌,等.巴马小型猪与人真皮的组织形态学和生物力学基本特征比较研究[J].第三军医大学学报,2010,32(08):754-758.
 Zheng Bixiang,Peng Daizhi,Zuo Haibin,et al.Comparative study on dermal histomorphologic and biomechanical properties between Bama miniature pig and human[J].J Third Mil Med Univ,2010,32(08):754-758.
点击复制

巴马小型猪与人真皮的组织形态学和生物力学基本特征比较研究(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第08期
页码:
754-758
栏目:
论著
出版日期:
2010-04-30

文章信息/Info

Title:
Comparative study on dermal histomorphologic and biomechanical properties between Bama miniature pig and human
作者:
郑必祥彭代智左海斌何斌王丽华何升东王勇周新刘敬
第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
Zheng Bixiang Peng Daizhi Zuo Haibin He Bin Wang Lihua He Shengdong Wang Yong Zhou Xin Liu Jin
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
真皮组织形态学基质生物力学
Keywords:
dermis histomorphology swine human matrix biomechanical properties
分类号:
R322.99;R318;R329.24
文献标志码:
A
摘要:
目的 通过比较研究巴马小型猪与人皮肤在真皮组织结构、胶原类型和生物力学性能方面的差异,寻找猪脱细胞真皮基质临床应用受限相关的组织结构及生物力学原因。 方法 收集巴马小型猪与人的背部全层皮肤样本,采用大体观察、HE染色、Masson三色染色、天狼猩红染色、VVG染色、扫描电镜及透射电镜等方法,显微照相后进行形态学观察和图像分析软件测量,同时利用材料试验机检测真皮生物力学性能。 结果 与人皮肤比较,光镜下可见巴马小型猪皮肤的真皮胶原纤维束更粗[(39.29±10.17)μm vs (17.21±5.20)μm] (P=0.000 3),而其间隙率更低[(19.01±1.55)% vs (32.36±1.28)%](P=0.029);真皮弹性纤维分布、Ⅰ型和Ⅲ型胶原含量及其比值也与人真皮有明显差异(P<0.05);扫描电镜显示巴马小型猪真皮的胶原纤维束排列紧密、束间孔隙小,透射电镜显示巴马小型猪真皮的胶原原纤维较细,横纹的周期更长;生物力学检测显示巴马小型猪真皮的最大应变[(0.52±0.05)mm/mm]低于人真皮[(0.80±0.06)mm/mm](P=0.003),而弹性模量[(69.65±12.16)MPa]和最大应力[(20.72±1.49)MPa]高于人真皮[分别为(44.23±4.63)、(11.85±0.19)MPa] (P=0.028,P=0.001)。 结论 巴马小型猪的真皮与人真皮差异较大,从组织形态学的角度提示猪脱细胞真皮基质不是修复人体真皮缺损的最佳生物材料。
Abstract:
Objective To compare the dermal tissue structure, content of difference types of collagen and biomechanical properties between Bama miniature pig and human skin, and to identify the histomorphologic reasons that limit the clinical application of porcine acellular dermal matrix.  Methods The dorsal fullthickness skin samples were collected from Bama miniature pig and human, and then observed grossly. The sections of these samples were stained by hematoxylin and eosin (HE) staining, Masson’s trichrome staining, sirius red staining and Verhoeff’s Van Gieson (VVG) staining, and were examined under light microscopy. These samples were also observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The relevant data of histological images were measured with image analysis software. In the meantime, these samples were detected by material testing machine for biomechanical properties.  Results Compared with human dermis, Bama miniature pig had much thicker dermal collagen bundles [(39.29±10.17)μm vs (17.21±5.20)μm, P=0.000 3], and had significant decrease in the gap rate between these bundles [(19.01±1.55]%  vs (32.36±1.28)%, P=0.029]. The dermal collagen bundles of Bama miniature pig were densely arranged and had smaller pores by SEM, the collagenous fibrils were thicker, and had longer cyclical transverse striation by TEM. The distribution of elastic fiber, the contents of type Ⅰ and Ⅲ collagen and their ratio also had significant differences between human and porcine dermis (P<0.05). The maximum strain of Bama miniature pig dermis (0.52±0.05)mm/mm was smaller than that of human dermis [(0.80±0.06)mm/mm, P=0.003], while the elastic modulus (69.65±12.16)MPa and maximum stress (20.72±1.49)MPa of Bama miniature pig dermis were stronger than that of human dermis (44.23±4.63) and (11.85±0.19)MPa (P=0.028, P=0.001).  Conclusion The dermis of pig and human are quite different. It is suggested that the acelluar dermal matrix from pig is not an optimal biomaterial for repairing and regenerating the human dermis. 

参考文献/References:

郑必祥,彭代智,左海斌,等. 巴马小型猪与人真皮的组织形态学和生物力学基本特征比较研究[J].第三军医大学学报,2010,32(8):754-758.

相似文献/References:

[1]钟华红,陈静嫦,吴河坪,等.肉毒杆菌毒素A水凝胶对大鼠骨骼肌远期作用的形态学观察[J].第三军医大学学报,2008,30(10):960.
 ZHONG Hua-hong,CHEN Jing-chang,WU He-ping,et al.Long-term morphologic changes in skeletal muscles of SD rats after botulinum toxin A gel injection[J].J Third Mil Med Univ,2008,30(08):960.
[2]柯宁,赵敏,李臻.胶联羊膜的组织形态、细胞相容性及抗拉力特性研究[J].第三军医大学学报,2009,31(04):322.
 KE Ning,ZHAO Min,LI Zhen.Histomorphology, cellular biocompatibility and tensile force resistance property of fibrin-binding amniotic membrane in vitro[J].J Third Mil Med Univ,2009,31(08):322.
[3]陈博,彭代智,左海斌,等.渗透冲击联合超声和去污剂处理对新生牛真皮组织学和生物活性成分的影响[J].第三军医大学学报,2012,34(09):797.
 Chen Bo,Peng Daizhi,Zuo Haibin,et al.Effect of osmotic shock combined with sonication and detergent treatment on histology and bioactive components of newborn bovine dermis[J].J Third Mil Med Univ,2012,34(08):797.
[4]陈亮,李晓格,毋巨龙,等.自体真皮移植矫正下眼睑凹陷的临床应用[J].第三军医大学学报,2013,35(02):172.
 Chen Liang,Li Xiaoge,Wu Julong,et al.Clinical application of autologous dermal transplantation to correct lower eyelid depression: report of 13 eyes[J].J Third Mil Med Univ,2013,35(08):172.
[5]杨东运,李世荣,毋巨龙,等.人皮肤真皮在增生性瘢痕形成中作用的实验研究[J].第三军医大学学报,2004,26(23):0.[doi:10.16016/j.1000-5404.2004.23.021 ]
 YANG Dong-yun,LI Shi-rong,WU Ju-long,et al.[J].J Third Mil Med Univ,2004,26(08):0.[doi:10.16016/j.1000-5404.2004.23.021 ]
[6]伍津津,朱堂友,鲁元刚,等.表皮真皮分离方法的探索[J].第三军医大学学报,2004,26(24):0.[doi:10.16016/j.1000-5404.2004.24.027 ]
 WU Jin-jin,ZHU Tang-you,LU Yuan-gang,et al.[J].J Third Mil Med Univ,2004,26(08):0.[doi:10.16016/j.1000-5404.2004.24.027 ]
[7]杨燕妮综述,彭代智,伍素华,等.天然真皮支架抗原性研究进展[J].第三军医大学学报,2004,26(24):0.[doi:10.16016/j.1000-5404.2004.24.037 ]
[8]史春梦,程天民.大鼠真皮多能间充质干细胞的分离培养[J].第三军医大学学报,2001,23(09):0.[doi:10.16016/j.1000-5404.2001.09.031 ]
 SHI Chun meng,CHENG Tian min.[J].J Third Mil Med Univ,2001,23(08):0.[doi:10.16016/j.1000-5404.2001.09.031 ]

更新日期/Last Update: 2010-04-28