[1]罗春山,田晓滨,汪雷,等.汉防己甲素对大鼠急性脊髓损伤后bcl-2和bax表达的影响[J].第三军医大学学报,2010,32(12):1321-1324.
 Luo Chunshan,Tian Xiaobin,Wang Lei,et al.Influence of tetrandrine on Bcl-2 and Bax expressions in rats with spinal cord injury[J].J Third Mil Med Univ,2010,32(12):1321-1324.
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汉防己甲素对大鼠急性脊髓损伤后bcl-2和bax表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第12期
页码:
1321-1324
栏目:
论著
出版日期:
2010-06-30

文章信息/Info

Title:
Influence of tetrandrine on Bcl-2 and Bax expressions in rats with spinal cord injury
作者:
罗春山田晓滨汪雷李波彭智周焯家简月奎赵伟峰
贵州省人民医院骨科
Author(s):
Luo Chunshan Tian Xiaobin Wang Lei Li Bo Peng Zhi Zhou Zuojia Jian Yuekui Zhao Weifeng
Department of Orthopaedics, People’s Hospital of Guizhou Province, Guiyang, Guizhou Province, 550001, China
关键词:
粉防己碱急性脊髓损伤基因bcl-2bcl-2相关X蛋白质
Keywords:
Tetrandrine acute spinal cord injuries genes bcl-2 bcl-2-associated X protein
分类号:
R285.5;R651.2;R363.25
文献标志码:
A
摘要:
目的    观察汉防己甲素(tetrandrine,Tet)对大鼠急性脊髓损伤后bcl-2和bax表达的影响,探讨其对脊髓损伤的作用机制。    方法    成年SD大鼠100只,分为4组:假手术组10只,损伤对照组、甲基强的松龙(methylprednisolone, MP)治疗组、Tet治疗组各30只。胸8、9椎板切除后,损伤对照组,MP、Tet治疗组用加速压迫型Allen’s打击法制成脊髓损伤模型,后2组动物于制模前,伤后24、48 h尾静脉分别注射MP 90 mg/kg和1% Tet 22.5 mg/kg。各组大鼠于术后8 h,1、3、7、14 d行运动功能BBB评分,取损伤段脊髓行石蜡切片HE染色,观察脊髓组织的形态结构变化,免疫组织化学染色检测细胞凋亡因子bcl-2、bax表达。    结果    伤后7、14 d MP、Tet治疗组大鼠运动功能评分显著高于损伤对照组(P<0.05),各时间点MP、Tet治疗组评分无统计学意义(P>0.05);MP、Tet治疗组脊髓组织损害较损伤对照组轻,术后8 h至14 d动态观察,3~7 d损伤表现最为严重,达到损伤高峰期;假手术组中bax、bcl-2阳性细胞数较少,MP、Tet治疗组bax阳性细胞数少于损伤对照组(P<0.05),而bcl-2阳性细胞数多于损伤对照组(P<0.05)。    结论    Tet可通过增加bcl-2表达和降低bax表达来抑制急性脊髓损伤后神经细胞的凋亡,有益于脊髓组织的保护,促进运动功能的恢复。
Abstract:
Objective    To observe the influence of tetrandrine (Tet) on the expressions of Bcl-2 and Bax in rats with spinal cord injury.     Methods    A total of 100 SD rats were randomly divided into sham-operation group (group A, n=10), spinal cord injury group (group B, n=30), methylprednisolone group (MP) group (group C, n=30) and Tet group (group D, n=30). The modified Allen method was adopted to establish the models of T9 spinal cord injury in groups B, C and D. The animals of group D received 1% 22.5 mg/kg Tet through tail vein in 24 and 48 h after operation, those of group B were injected with normal saline at same volume as in group D, and the rats from group C were given a tail vein injection of  90 mg/kg MP before and 24 and 48 h after injury. BBB score was recorded at  8 h, and 1, 3,7 and 14 d after operation . Six rats from each group were killed immediately after BBB scoring, and the samples were taken from the spinal cord injury sites for HE staining. The expression levels of Bcl-2 and Bax proteins in the obtained samples were studied with immunohistochemical assay.     Results    The BBB score of groups C and D were significantly higher than that of group B at day 7 and 14 (P<0.05). There was no significant difference in the score between groups C and D at each time points (P>0.05). HE staining showed the spinal cord of groups C and D had fewer necrosis than that of group B (P<0.05). The injury was most severe in day 3 to 7. The number of Bcl-2 positive cells in groups C and D were larger than those of group B at each time points (P<0.05). However the number of Bax postive cells in groups C and D were less than those of group B at each time points (P<0.05).     Conclusion    Tet is able to protect neurons from apoptosis and promote the nerve function recovery by inhibiting the expression of Bax and promoting the expression of bcl-2.

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更新日期/Last Update: 2010-06-22