[1]徐述雄,傅晓岚,孙兆林,等.IL-15诱导CD4+CD25- T细胞向CD4+CD25+调节性T细胞转化的机制探讨[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):759-762.
 Xu Shuxiong,Fu Xiaolan,Sun Zhaolin,et al.IL-15 converts CD4+CD25- T cells into CD4+CD25+ regulatory T cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):759-762.
点击复制

IL-15诱导CD4+CD25- T细胞向CD4+CD25+调节性T细胞转化的机制探讨(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第08期
页码:
759-762
栏目:
论著
出版日期:
2010-04-30

文章信息/Info

Title:
IL-15 converts CD4+CD25- T cells into CD4+CD25+ regulatory T cells
作者:
徐述雄傅晓岚孙兆林李晓伟陈丽萍刘宏吴雄飞
第三军医大学:西南医院肾科,全军免疫学研究所;贵州省人民医院泌尿外科
Author(s):
Xu Shuxiong Fu Xiaolan Sun Zhaolin Li Xiaowei Chen Liping Liu Hong Wu Xiongfei
Department of Kidney, Southwest Hospital, Institute of Immunology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038; Department of Urology, Guizhou Provincial People’s Hospital, Guiyang, Guizhou Province, 550002, China
关键词:
白介素-15调节性T细胞T细胞p-STAT5
Keywords:
IL-15regulatory T cellsT cellsp-STAT5
分类号:
R392-33;R392.11;R392.2
文献标志码:
A
摘要:
目的 初步探讨IL-15诱导CD4+ CD25-T细胞向CD4+ CD25+调节性T细胞(Tregs)转化的机制。 方法 采用免疫磁性细胞分离法分离人外周血中的CD4+CD25-T细胞,依据加入细胞因子不同分为4组:对照组、IL-15组、拮抗1组、拮抗2组,其中后3组均于培养的第1天加入IL-15,而拮抗1组和拮抗2组分别于培养第1、3天加入STAT5a封闭性肽。流式细胞仪检测细胞表型变化,3H-TdR掺入法检测IL-15诱导产生的Tregs抑制CD4+CD25-效应T细胞(Teff)增殖的免疫功能,Western blot法检测细胞内p-STAT5的变化。 结果 与对照组比较,IL-15能诱导CD4+CD25-T细胞中CD25和Foxp3表达上调,IL-15诱导生成的Tregs具有较天然Tregs稍弱的免疫抑制功能。Western blot检测结果显示,IL-15组p-STAT5表达较对照组明显上调。加入STAT5a封闭性肽后,拮抗1组和拮抗2组中CD4+CD25-T细胞中CD25和Foxp3表达下调,但拮抗2组[细胞表型分别为(47.9±6.0)%、(20.7±3.4)%]改变较拮抗1组[细胞表型分别为(30.7±8.5)%、(9.2±2.2)%]改变轻微。 结论 IL-15能诱导CD4+CD25-T细胞向Tregs转化,其机制可能是上调p-STAT5的表达。
Abstract:
Objective To investigate the effect and mechanism of IL-15 converting CD4+CD25- T cells into CD4+CD25+ regulatory T cells (Tregs).  Methods CD4+CD25- T cells were isolated from human peripheral blood mononuclear cells in 2 steps by magnetic cell sorting system. The purity rate of the sorted cells was measured by flow cytometry (FCM). CD4+CD25- T cells were divided into 4 groups: control group (without cytokines), IL-15 group (IL-15), antagonism I group (IL-15 and STAT5a blocking peptide was added at the first day) and Antagonism Ⅱ group (IL-15 was added at the first day, but STAT5a blocking peptide at the third day). FCM was used to detect the changes of CD4+CD25- T cells, the incorporation of [3H]thymidine was used to evaluate the suppressive function of the IL-15-induced Tregs (iTregs) on the Teff and Western blot analysis was used to observe the expression of p-STAT5.  Results Compared with CD4+CD25- T cells cultured without cytokines, IL-15 was able to promote CD25 and Foxp3 expression of CD4+CD25- T cells and IL-15induced CD4+CD25+ regulatory T cells exerted weak suppressor activity. Addition of IL-15 up-regulated the expression of p-STAT5 in CD4+CD25- T cells significantly. After STAT5a blocking peptide was added, IL-15-induced CD25 and Foxp3 expression of CD4+CD25-T cells was significantly down-regulated.  Conclusion IL-15 significantly converts naive CD4+ CD25-T cells into CD4+CD25+ regulatory T cells in vitro and up-regulated p-STAT5 may be involved in this process.

相似文献/References:

[1]吴奎,毕玉田,王耀丽,等.Foxp3基因转染对人CD4+ CD25-T细胞表型和功能的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):186.
 WU Kui,BI Yu-tian,WANG Yao-li,et al.Changes of phenotype and function of human CD4+CD25- T cells induced after Foxp3 transfection[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(08):186.
[2]张莹,姚咏明,董宁,等.高迁移率族蛋白B1对小鼠调节性T细胞Foxp3表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(21):1980.
 ZHANG Ying,YAO Yong-ming,DONG Ning,et al.Effect of high mobility group box-1 protein on Foxp3 expression in spleen regulatory T cells in mice[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(08):1980.
[3]魏秀丽,黄志,李欣,等.Foxp3基因及调节性T细胞在重症肌无力被动转移幼鼠发病中的作用机制[J].陆军军医大学学报(原第三军医大学学报),2010,32(04):353.
 Wei Xiuli,Huang Zhi,Li Xin,et al.Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):353.
[4]杜建新,蒋悍东,杜春华,等.PDGF、foxp3和MMP-9与哮喘气道重塑的关系研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(21):2329.
 Du Jianxin,Jiang Handong,Du Chunhua,et al.Relation between platelet-derived growth factor, foxp3, MMP-9 and airway remodeling due to asthma[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):2329.
[5]熊慧娟,周昌菊,杨益民,等.CD4+ CD25+ Foxp3+ 调节性T细胞在足月分娩发动中的变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(22):2438.
[6]冯林,刁庆春,郑峻松,等.MRL/lpr小鼠中CD4+CD25+调节性T细胞含量、增殖能力及抑制能力的研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(04):353.
 Feng Lin,Diao Qingchun,Zheng Junsong,et al.Population, proliferative ability and suppressive activity of CD4+CD25+ regulatory T cells in MRL/lpr mice[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(08):353.
[7]何跃,韩容,刘宏,等.Herpes virus entry mediator/CD160介导人调节性T细胞对CD4+效应性T细胞的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):1012.
 He Yue,Han Rong,Liu Hong,et al.Human regulatory T cells exert inhibitory function on CD4+ effector T cells through interaction between herpes virus entry mediator and CD160[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(08):1012.
[8]雷晓,余佩武,赵永亮,等.CD4+CD25+调节性T细胞在胃癌患者的表达及其临床意义[J].陆军军医大学学报(原第三军医大学学报),2006,28(05):397.
[9]姜锐,杨鹏,江亚萍.TNFR2基因敲除小鼠H22移植瘤生长特点和Treg数量与功能检测[J].陆军军医大学学报(原第三军医大学学报),2016,38(01):50.
 Jiang Rui,Yang Peng,Jiang Yaping.Tumor growth characteristics in H22 hepatocarcinoma cells transplanted tumor and number and function of Treg in TNFR2 knockout mice[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(08):50.
[10]曾冬竹,王自强,郑峻松,等.IL-2和IL-10对Treg细胞体外扩增影响的差异研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):290.
 ZENG Dong-zhu,WANG Zi-qiang,ZHENG Jun-song,et al.Effects of IL-2 and IL-10 on T regulatory cell proliferation in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):290.

更新日期/Last Update: 2010-04-28