[1]李佳霖,李媛,晁帆,等.法尼醇X受体对HepG2细胞蛋白C表达的影响及机制的初步研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1267-1270.
 Li Jialin,Li Yuan,Chao Fan,et al.Effects of farnesoid X receptor activation on human protein C expression in HepG2 cells and its possible mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1267-1270.
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法尼醇X受体对HepG2细胞蛋白C表达的影响及机制的初步研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第12期
页码:
1267-1270
栏目:
论著
出版日期:
2010-06-30

文章信息/Info

Title:
Effects of farnesoid X receptor activation on human protein C expression in HepG2 cells and its possible mechanism
作者:
李佳霖李媛晁帆龚薇许志臻何凤田
第三军医大学基础医学部生物化学与分子生物学教研室
Author(s):
Li Jialin Li Yuan Chao Fan Gong Wei Xu Zhizhen He Fengtian
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
关键词:
法尼醇X受体鹅脱氧胆酸蛋白质C肿瘤细胞培养的HepG2细胞
Keywords:
farnesoid X receptorchenodeoxycholic acidprotein C tumor cells cultured
分类号:
R394-33;R394.2;R575.1
文献标志码:
A
摘要:
目的    观察法尼醇X受体(farnesoid X receptor,FXR)的天然激动剂鹅脱氧胆酸(chenodeoxycholic acid,CDCA)对HepG2细胞蛋白C(protein C,PC)表达的影响,探讨其引起变化的可能机制。    方法    以不同浓度(25、50、75 μmol/L)FXR激动剂CDCA刺激HepG2细胞,RT-PCR分别检测各组细胞FXR特异性靶基因小异源二聚体伴侣分子 (small heterodimer partner,SHP)及PC mRNA表达变化,并用实时荧光定量PCR(real-time PCR)分析各组PC的表达量;ELISA检测各组细胞培养上清液中PC蛋白分泌量的变化;瞬时转染结合报告基因检测在人胚肝L02细胞中转入组成性活化的FXR表达质粒(VP-FXR),观察PC启动子活性的改变。    结果    经FXR配体CDCA刺激的HepG2细胞,随着CDCA浓度的增高,SHP、PC mRNA的表达逐渐增高,细胞培养上清液中PC蛋白表达也逐渐升高,呈剂量依赖性;在人胚肝L02细胞中,活化的FXR可增强PC启动子的活性约2.9倍。    结论    CDCA活化FXR引起HepG2细胞中PC mRNA和蛋白表达的升高,其调控机制与PC启动子的活化相关。
Abstract:
Objective    To investigate the effect of chenodeoxycholic acid (CDCA,the natural agonist of nuclear receptor,farnesoid X receptor, FXR ) on the expression of human protein C (PC) in HepG2 cells and the underlying related mechanism.      Methods    After HepG2 cells were treated with FXR agonist CDCA at the doses of 25, 50 or 75 μmol/L for 24 h, RT-PCR was used to detect the mRNA expressions of small heterodimer partner (SHP) and PC, and real-time PCR was employed for PC mRNA expression. ELISA was used to detect the concentration of PC in the supernatants of HepG2 cells. Transient transfection Luciferase report assay was performed to analyze the change of PC promoter activity after cotansfecting the plasmid VP-FXR expressing activated FXR into human embryo hepatocyte L02 cells.     Results    CDCA remarkably elevated SHP and PC mRNA expressions, and PC concentration in the supernatants in a dose-dependent fashion.  CDCA at 75 μmol/L increased the amount of PC secretion by (4.22±0.97) fold in comparison to those treated by DMSO. Activated FXR enhanced the activity of PC promoter up to about 2.90 fold comparing with control group in L02 cells.      Conclusion    CDCA can activate FXR, and result in the up-regulation of PC in HepG2 cells. The underlying mechanism may be involved in the activation of PC promoter.

相似文献/References:

[1]王永超,刘红,彭家和,等.法尼酯X受体上调肝细胞中成纤维细胞生长因子21的表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):912.
 Wang Yongchao,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor upregulates fibroblast growth factor 21 expression in cultured liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):912.
[2]刘小花,王永超,李良鹏,等.CDCA在C57BL/6小鼠中上调肝脏成纤维细胞生长因子21表达及其效应[J].陆军军医大学学报(原第三军医大学学报),2012,34(18):1831.
 Liu Xiaohua,Wang Yongchao,Li Liangpeng,et al.Chenodesoxycholic acid up-regulates fibroblast growth factor 21 and its effect on C57BL/6 mice[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(12):1831.

更新日期/Last Update: 2010-06-22