[1]张园园,胡国华,袁琨,等.SDF-1/CXCR4对喉癌细胞归巢的作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1316-1320.
 Zhang Yuanyuan,Hu Guohua,Yuan Kun,et al.Effect of SDF-1/CXCR4 axis to homing of laryngeal carcinoma cell Hep-2[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1316-1320.
点击复制

SDF-1/CXCR4对喉癌细胞归巢的作用(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第12期
页码:
1316-1320
栏目:
论著
出版日期:
2010-06-30

文章信息/Info

Title:
Effect of SDF-1/CXCR4 axis to homing of laryngeal carcinoma cell Hep-2
作者:
张园园胡国华袁琨但洋
武汉市中心医院耳鼻咽喉科;重庆医科大学附属第一医院耳鼻咽喉科;武汉市第三医院整形科
Author(s):
Zhang Yuanyuan Hu Guohua Yuan Kun Dan Yang
Department of Otolaryngology, Central Hospital of Wuhan, Wuhan, Hubei Province, 430030; Department of Otolaryngology, First Affiliated Hospital, Chongqing Medical University, 400016; Department of Plastic Surgery, Third Hospital of Wuhan, Wuhan, Hubei Province, 430060, China
关键词:
细胞系肿瘤喉肿瘤血管内皮生长因子-C受体CXCR4趋化因子CXCL12
Keywords:
cell line tumor laryngeal neoplasms vascular endothelial growth factor C receptors CXCR4 chemokine CXCL12
分类号:
R73-37;R739.65;R394-33
文献标志码:
A
摘要:
目的    在体外探讨血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)、趋化因子受体(Cys-X-Cys receptor 4,CXCR4)及其配体基质细胞衍生因子-1(stromal cell-derived factor 1,SDF-1)在喉癌Hep-2细胞株中的表达及其在喉癌细胞归巢中的作用。    方法    ①分别用RT-PCR及Western blot检测喉癌Hep-2细胞株中CXCR4 mRNA和蛋白的表达和重组人SDF-1与CXCR4特异性拮抗剂(AMD3100)作用前后喉癌Hep-2细胞株中VEGF-C mRNA和蛋白表达水平的变化;②用甲基噻唑基四唑(MTT比色法)测定不同浓度重组人SDF-1以及AMD3100作用前后Hep-2细胞增殖率的变化;③Transwell小室迁移及体外侵袭实验检测不同浓度重组人SDF-1以及AMD3100对Hep-2细胞趋化活性的影响。    结果    ①在mRNA以及蛋白水平,CXCR4及VEGF-C在Hep-2细胞株中均有高表达,在不同浓度重组人SDF-1(1、10、100 ng/ml)作用Hep-2细胞株24 h后,VEGF-C的表达明显上调,且有明显的剂量依赖性,并能被1 μg/ml的CXCR4的特异性拮抗剂AMD3100阻断;②与对照组(未处理的Hep-2细胞)和10 ng/ml SDF-1组比较,100 ng/ml SDF-1组有明显促进喉癌细胞增殖的作用(P<0.05),并能被1 μg/ml的AMD3100阻断;③与对照组和10 ng/ml SDF-1组比较,100 ng/ml SDF-1组同样有明显的促喉癌细胞迁移和侵袭的作用(P<0.05),同样也能被1 μg/ml的AMD3100所阻断。    结论    SDF-1/CXCR4生物轴与VEGF-C协同促进喉癌细胞归巢,可作为干预喉癌淋巴转移的新手段。
Abstract:
Objective    To investigate the in vitro expressions of chamotatic factor CXCL12 [also known as stromal cell-derived factor 1 (SDF-1)], the receptor of the factor, Cys-X-Cys receptor 4 (CXCR4) and vascular endothelial growth factor C (VEGF-C) in human laryngeal carcinoma cell line Hep-2 and their roles in the homing of laryngeal carcinoma.      Methods    The expressions of CXCR4 mRNA and protein in Hep-2 cells was detected by RT-PCR and Western blotting, the expression of VEGF-C also was evaluated before and after incubated with SDF-1 (1, 10, 100 ng/ml) or the antagonist of CXCR4 AMD3100. Methythiazolyltetrazolium (MTT) assay was used to analyze the effect of different concentrations of SDF-1 on the proliferation of Hep-2 cells. Transwell invasion chamber and matrigel were used to evaluate the effect of various concentrations of SDF-1 and AMD3100 on the migration and invasion of Hep-2 cells.     Results    1)The CXCR4 and VEGF-C were both overexpressed at mRNA and protein level in Hep-2 cells, and the expression of VEGF-C in Hep-2 cells was upregulated with a concentration-dependent model, which was inhibited by CXCR4 antagonist (P<0.05). 2)SDF-1(100 ng/ml) significantly enhanced the proliferation of Hep-2 cells compared with the control and 10 ng/ml SDF-1 groups (24 h: 0.585±0.051 vs 0.425±0.045 and 0.428±0.039, P<0.05; 48 h: 0.631±0.062 vs 0.497±0.067  and 0.532±0.039, P<0.01). This enhancing effect of SDF-1 was significantly inhibited by 1 μg/ml AMD3100. 3)The levels of migration and invasion of the Hep-2 cells treated with 100 ng/ml SDF-1 were significantly higher than those in the control and 10 ng/ml SDF-1 (migration: 286.7±24.7 vs 48.0±7.2 and 147.7±37.0; invasion: 40.5±4.5 vs 4.0±1.0 and 17.7±2.0, P<0.05), and was strongly inhibited by 1 μg/ml AMD3100.      Conclusion    SDF-1/CXCR4 can in coordination with VEGF-C promote homing of laryngeal carcinoma cell line Hep-2, and may be a new pathway to prevent laryngeal carcinoma lymph node metastasis. 

相似文献/References:

[1]卞修武.对肿瘤研究前沿之肿瘤干细胞的几点认识[J].陆军军医大学学报(原第三军医大学学报),2008,30(11):995.
[2]林爽,李力,易萍.低氧对宫颈癌HeLa细胞黏附及侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(18):1776.
 LIN Shuang,LI Li,YI Ping.Effect of hypoxia on adhesion and invasion of human cervical cancer cell HeLa[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(12):1776.
[3]王东.肿瘤分子病理和分子靶向治疗进展[J].陆军军医大学学报(原第三军医大学学报),2006,28(02):91.
[4]王群波,蒋电明,李智,等.纳米羟基磷灰石/聚酰胺66复合人工椎体治疗胸腰椎椎体肿瘤的效果[J].陆军军医大学学报(原第三军医大学学报),2006,28(03):263.
[5]李明,周永,糜漫天.染料木黄酮对人乳腺癌细胞株MDA-MB-453、MCF-7、MCF-7/HER2增殖能力的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1618.
 LI Ming,ZHOU Yong,MI Man-tian.Effect of genistein on proliferation of human breast cancer cell lines MDA-MB-453, MCF-7 and MCF-7/HER2[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1618.
[6]仲召阳,王东,李增鹏,等.多肿瘤标志物蛋白质芯片检测对肿瘤诊断的临床意义[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1658.
 ZHONG Zhao-yang,WANG Dong,LI Zeng-peng,et al.C-12 multiple tumor marker protein chip detection system in diagnosis of tumor[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1658.
[7]余佩武,赵永亮.树突状细胞抗肿瘤作用研究进展[J].陆军军医大学学报(原第三军医大学学报),2006,28(05):381.
[8]刘宿,蒋耀光,葛衡江,等.肺癌食管癌开胸手术患者术中凝血功能改变及其临床意义[J].陆军军医大学学报(原第三军医大学学报),2007,29(09):779.
 LIU Su,JIANG Yao-guang,GE Heng-jiang,et al.Changes and clinical significance of coagulation function of patients with lung cancer or esophagus cancer in thoracotomy[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(12):779.
[9]张国桥,凌贤龙,陈正堂.线粒体DNA缺失肝癌细胞内活性氧、线粒体膜电位变化实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):711.
 ZHANG Guo-qiao,LING Xian-long,CHEN Zheng-tang.Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(12):711.
[10]王静,刘亮,李金梅,等.青蒿琥酯抗人食管癌作用与调控CDC25A表达的关系[J].陆军军医大学学报(原第三军医大学学报),2007,29(05):428.
 WANG Jing,LIU Liang,LI Jin-mei,et al.Inhibitory effect of artesunate on human esophageal carcinoma is associated with CDC25A modulation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(12):428.
[11]辇伟奇,敖绪军,陈芳琳,等.CXCR4阳性Lewis肺癌细胞的高致瘤性和高转移潜能[J].陆军军医大学学报(原第三军医大学学报),2010,32(04):315.
 Nian Weiqi,Ao Xujun,Chen Fanglin,et al.Highly tumorigenic and metastatic potential of CXCR4 positive cells from Lewis lung carcinoma cell line[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):315.
[12]毛丽伟,廖国清,王红梅,等.放射控导的wt-p53蛋白正反馈基因环路对肺腺癌细胞周期、凋亡及放射敏感性的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):462.
 Mao Liwei,Liao Guoqing,Wang Hongmei,et al.Effect of irradiation-induced wt-p53 positive circuit on cell life cycle, apoptosis and irradiation sensitivity of lung adenocarcinoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):462.
[13]杨镇洲,张云嵩,李梦侠,等.血卟啉衍生物介导的光动力疗法对人肺腺癌细胞杀伤效应的实验研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):494.
[14]刘海忠,于超,杨竹,等.土贝母皂苷甲增强人卵巢癌A2780/DDP细胞对顺铂的敏感性[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):695.
 Liu Haizhong,Yu Chao,Yang Zhu,et al.Tubeimoside 1 enhances chemosensitivity of drug-resistant ovarian cancer A2780/DDP cells to cisplatin[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):695.
[15]史良会,张才全.γ-氨基丁酸及其A型受体在胃癌SGC-7901细胞的表达及对细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(20):2132.
 Shi Lianghui,Zhang Caiquan.Expression of GABA and GABRP in gastric cancer SGC-7901 cells and its effect on cell migration ability[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):2132.
[16]徐高峰,王茂德,谢万福,等.沉默缺氧诱导因子对人胶质瘤SHG44细胞糖酵解及细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(20):2148.
 Xu Gaofeng,Wang Maode,Xie Wanfu,et al.Reversal of glycolytic phenotype by HIF-1α siRNA inhibits SHG44 cell growth in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):2148.
[17]潘琪,周强,高志棣,等.去甲斑蝥素体外抑制NCI-H446细胞迁移、增殖及促进凋亡[J].陆军军医大学学报(原第三军医大学学报),2011,33(20):2211.
[18]陈杰,庞江琳,覃燕梅,等.半边旗有效成分5F对人小细胞肺癌NCIH446细胞毒性、侵袭能力、运动能力及MMP-9、uPA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(04):361.
[19]陈振波,赵俪梅,罗艳梅,等.共培养体系中卵巢癌细胞对内皮祖细胞成管及MMP-2和MMP-9表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):956.
 Chen Zhenbo,Zhao Limei,Luo Yanmei,et al.Effect of ovarian cancer cells on MMP-2 and MMP-9 expression and tube formation of endothelial progenitor cells in co-culture system[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(12):956.

更新日期/Last Update: 2010-06-22