[1]张园园,胡国华,袁琨,等.SDF-1/CXCR4对喉癌细胞归巢的作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1316-1320.
　Zhang Yuanyuan,Hu Guohua,Yuan Kun,et al.Effect of SDF-1/CXCR4 axis to homing of laryngeal carcinoma cell Hep-2[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1316-1320.
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SDF-1/CXCR4对喉癌细胞归巢的作用(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第12期
页码:
1316-1320
栏目:
论著
出版日期:
2010-06-30

文章信息/Info

Title:
Effect of SDF-1/CXCR4 axis to homing of laryngeal carcinoma cell Hep-2
作者:
张园园; 胡国华; 袁琨; 但洋
武汉市中心医院耳鼻咽喉科;重庆医科大学附属第一医院耳鼻咽喉科;武汉市第三医院整形科
Author(s):
Zhang Yuanyuan;  Hu Guohua;  Yuan Kun;  Dan Yang
Department of Otolaryngology, Central Hospital of Wuhan, Wuhan, Hubei Province, 430030; Department of Otolaryngology, First Affiliated Hospital, Chongqing Medical University, 400016; Department of Plastic Surgery, Third Hospital of Wuhan, Wuhan, Hubei Province, 430060, China
关键词:
细胞系; 肿瘤; 喉肿瘤; 血管内皮生长因子-C; 受体; CXCR4; 趋化因子CXCL12
Keywords:
cell line;  tumor;  laryngeal neoplasms;  vascular endothelial growth factor C;  receptors;  CXCR4;  chemokine CXCL12
分类号:
R73-37;R739.65;R394-33
文献标志码:
A
摘要:
目的    在体外探讨血管内皮生长因子C（vascular endothelial growth factor C，VEGF-C）、趋化因子受体(Cys-X-Cys receptor 4，CXCR4)及其配体基质细胞衍生因子-1(stromal cell-derived factor 1，SDF-1)在喉癌Hep-2细胞株中的表达及其在喉癌细胞归巢中的作用。    方法    ①分别用RT-PCR及Western blot检测喉癌Hep-2细胞株中CXCR4 mRNA和蛋白的表达和重组人SDF-1与CXCR4特异性拮抗剂（AMD3100）作用前后喉癌Hep-2细胞株中VEGF-C mRNA和蛋白表达水平的变化；②用甲基噻唑基四唑（MTT比色法）测定不同浓度重组人SDF-1以及AMD3100作用前后Hep-2细胞增殖率的变化；③Transwell小室迁移及体外侵袭实验检测不同浓度重组人SDF-1以及AMD3100对Hep-2细胞趋化活性的影响。    结果    ①在mRNA以及蛋白水平，CXCR4及VEGF-C在Hep-2细胞株中均有高表达，在不同浓度重组人SDF-1（1、10、100 ng/ml）作用Hep-2细胞株24 h后，VEGF-C的表达明显上调，且有明显的剂量依赖性，并能被1 μg/ml的CXCR4的特异性拮抗剂AMD3100阻断；②与对照组(未处理的Hep-2细胞)和10 ng/ml SDF-1组比较，100 ng/ml SDF-1组有明显促进喉癌细胞增殖的作用（P<0.05），并能被1 μg/ml的AMD3100阻断；③与对照组和10 ng/ml SDF-1组比较，100 ng/ml SDF-1组同样有明显的促喉癌细胞迁移和侵袭的作用（P<0.05),同样也能被1 μg/ml的AMD3100所阻断。    结论    SDF-1/CXCR4生物轴与VEGF-C协同促进喉癌细胞归巢，可作为干预喉癌淋巴转移的新手段。
Abstract:
Objective    To investigate the in vitro expressions of chamotatic factor CXCL12 [also known as stromal cell-derived factor 1 (SDF-1)], the receptor of the factor, Cys-X-Cys receptor 4 (CXCR4) and vascular endothelial growth factor C (VEGF-C) in human laryngeal carcinoma cell line Hep-2 and their roles in the homing of laryngeal carcinoma.      Methods    The expressions of CXCR4 mRNA and protein in Hep-2 cells was detected by RT-PCR and Western blotting, the expression of VEGF-C also was evaluated before and after incubated with SDF-1 (1, 10, 100 ng/ml) or the antagonist of CXCR4 AMD3100. Methythiazolyltetrazolium (MTT) assay was used to analyze the effect of different concentrations of SDF-1 on the proliferation of Hep-2 cells. Transwell invasion chamber and matrigel were used to evaluate the effect of various concentrations of SDF-1 and AMD3100 on the migration and invasion of Hep-2 cells.     Results    1)The CXCR4 and VEGF-C were both overexpressed at mRNA and protein level in Hep-2 cells, and the expression of VEGF-C in Hep-2 cells was upregulated with a concentration-dependent model, which was inhibited by CXCR4 antagonist (P<0.05). 2）SDF-1(100 ng/ml) significantly enhanced the proliferation of Hep-2 cells compared with the control and 10 ng/ml SDF-1 groups (24 h: 0.585±0.051 vs 0.425±0.045 and 0.428±0.039, P<0.05; 48 h: 0.631±0.062 vs 0.497±0.067  and 0.532±0.039, P<0.01). This enhancing effect of SDF-1 was significantly inhibited by 1 μg/ml AMD3100. 3)The levels of migration and invasion of the Hep-2 cells treated with 100 ng/ml SDF-1 were significantly higher than those in the control and 10 ng/ml SDF-1 (migration: 286.7±24.7 vs 48.0±7.2 and 147.7±37.0; invasion: 40.5±4.5 vs 4.0±1.0 and 17.7±2.0, P<0.05), and was strongly inhibited by 1 μg/ml AMD3100.      Conclusion    SDF-1/CXCR4 can in coordination with VEGF-C promote homing of laryngeal carcinoma cell line Hep-2, and may be a new pathway to prevent laryngeal carcinoma lymph node metastasis. 

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更新日期/Last Update: 2010-06-22