[1]张政,罗勇军,彭惠民,等.早期糖尿病肾病相关微小RNA在小鼠肾脏的表达变化[J].第三军医大学学报,2010,32(13):1383-1386.
　Zhang Zheng,Luo Yongjun,Peng Huimin,et al.Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice[J].J Third Mil Med Univ,2010,32(13):1383-1386.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第13期
页码:
1383-1386
栏目:
论著
出版日期:
2010-07-15

文章信息/Info

Title:
Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice
作者:
张政; 罗勇军; 彭惠民; 何晓燕
重庆医科大学：细胞生物学及遗传学教研室,分子医学与肿瘤研究中心,实验教学中心;第三军医大学高原军事医学系病理生理学教研室
Author(s):
Zhang Zheng;  Luo Yongjun;  Peng Huimin;  He Xiaoyan
Department of Cell Biology and Genetics, College of Basic Medical Sciences, Center of Molecular Medicine and Cancer Research，4Experimental Teaching Center, Chongqing Medical University, Chongqing, 400016; Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine，Third Military Medical University，Chongqing, 400038, China
关键词:
糖尿病肾病; microRNA; microRNA芯片; 差异表达
Keywords:
diabetic nephropathy; microRNA; microRNA microarray; differential expression
分类号:
R394.2
文献标志码:
A
摘要:
目的    建立db/db小鼠早期糖尿病肾病（diabetic nephropathy，DN）微小RNA（microRNA，miRNA）的差异表达谱。    方法    采用12只9周龄高血糖且24 h尿白蛋白显著增高的db/db小鼠作为早期糖尿病肾病组，12只9周龄db/m小鼠作为正常对照组，用于miRNA芯片和实时荧光定量RT-PCR检测。Trizol法抽提肾脏总RNA，YM-100微离心滤器富集小RNA，芯片技术检测DN组和对照组miRNAs的差异表达谱。应用实时荧光定量RT-PCR验证部分差异miRNAs，运用生物信息学技术预测部分相关miRNAs的靶基因。     结果    芯片结果显示，66个miRNA在DN组和对照组存在差异表达（P<0.01），其中35个miRNAs在DN组呈高表达，31个miRNAs在DN组呈低表达，差异倍数从1.08~10.20。实时荧光定量RT-PCR进一步验证部分miRNA的表达，结果显示3个miRNAs在DN组表达上调，分别为：miR-196a、miR-98和miR-29c；4个表达在DN组下调，分别为miR-21、miR-451、miR-709和miR-187，差异显著（P<0.01）。    结论    部分miRNAs在DN和正常对照肾脏组织中存在差异表达，可能参与了DN发生的分子机制。
Abstract:
Objective    To establish the differential expression profile of early diabetic nephropathy (DN)- related microRNA (miRNA) in kidneys of db/db mice.     Methods    Twelve 9-week-old male db/db mice with hyperglycemia and significantly elevated urinary albumin excretion were used as an early DN group，and another twelve 9-week-old male db/m mice were used as a control group. Total mRNA was extracted from kidney tissues of mice and miRNA was concentrated with the YM-100 micro-centrifugation filter. Differential expression profile of miRNA in DN and control groups was detected by miRNA assay and RT-PCR, respectively. Target gene of related miRNA was detected with bioinformatics technology.     Results    miRNA assay showed that 66 miRNAs were differentially expressed in DN and control groups (P<0.01). Of the 66 miRNAs, 35 and 31 were highly and lowly expressed, respectively, in DN group, with a difference of 1.08 to 10.20 folds. RT-PCR showed that the expression of 3 miRNAs was up-regulated (miR-196a, miR-98 and miR-29c) and the expression of 4 miRNAs was down-regulated (miR-21, miR-451, miR-709 and miR-187) in DN group（P<0.01）.     Conclusion    Some miRNAs are differentially expressed in the kidney tissues of DN and normal mice, suggesting that miRNAs may be involved in the molecular mechanism of DN.

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更新日期/Last Update: 2010-07-09