[1]董金瑜,刘红,彭家和,等.法尼酯X受体可下调肝细胞中肝型脂肪酸结合蛋白的表达[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):458-461.
　Dong Jinyu,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor down-regulates expression of liver fatty acid-binding protein in liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(05):458-461.
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第05期
页码:
458-461
栏目:
论著
出版日期:
2010-03-15

文章信息/Info

Title:
Farnesoid X receptor down-regulates expression of liver fatty acid-binding protein in liver cells
作者:
董金瑜; 刘红; 彭家和; 张艳; 王强; 李良鹏; 王永超; 江渝
第三军医大学基础医学部生物化学与分子生物学教研室;第三军医大学新桥医院血液科
Author(s):
Dong Jinyu;  Liu Hong;  Peng Jiahe;  Zhang Yan;  Wang Qiang; Li Liangpeng;  Wang Yongchao;  Jiang Yu
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038; Department of Hematology, Xinqiao Hospital, Third Military Medical University,Chongqing, 400037, China
关键词:
法尼酯X受体; 肝型脂肪酸结合蛋白; 肝细胞
Keywords:
farnesoid X receptor;  liver fatty acid-binding protein;  ;  hepatocytes
分类号:
R333.4;R341;R394.2
文献标志码:
A
摘要:
目的  研究法尼酯X受体( farnesoid X receptor，FXR )对肝型脂肪酸结合蛋白（liver fatty acid-binding protein，L-FABP）表达的影响。  方法  用终浓度为50、100 μmol/L的鹅脱氧胆酸（chenodesoxycholic acid，CDCA）以及1、5 μmol/L 的GW4064分别处理人胚胎肝细胞LO2和人肝癌细胞株HepG2后，用逆转录-聚合酶链反应（RT-PCR）法检测FXR特异靶基因小异二聚体配体（small heterodimer partner，SHP）和L-FABP mRNA表达，再用Western blot法检测L-FABP 蛋白水平的变化。  结果  LO2和HepG2细胞经FXR特异性激动剂CDCA以及GW4064刺激后，细胞内的SHP mRNA 水平均明显升高，表明FXR在这两种细胞中是有功能活性的；而L-FABP mRNA和蛋白水平显著下降（P<0.05）。  结论  激活后的FXR可抑制LFABP的表达活性。
Abstract:
Objectives  To investigate the effect of farnesoid X receptor (FXR) on the expression of liver fatty acidbinding protein (L-FABP).   Methods  Human hepatoblastoma HepG2 cells and hepatocyte LO2 cells were treated with FXR agonists chenodesoxycholic acid (CDCA）at 50 and 100 μmol/L or GW4064 at 1 and 5 μmol/L respectively. The L-FABP and the specific gene of FXR-small heterodimer partner (SHP) were detected by reverse transcription polymerase chain reaction (RT-PCR). The L-FABP protein level was determined by Western blotting.   Results  After treated with CDCA or GW4064, SHP mRNA level in HepG2 and LO2 was increased obviously, which suggested FXR was functional in those cells. The mRNA and protein levels of L-FABP was decreased dramatically in those cells (P<0.05).   Conclusion  Farnesoid X receptor down-regulates the expression of L-FABP in hepatocytes.

相似文献/References:

[1]申丽丽,彭家和,刘红,等.法尼酯X受体对脂肪酸合酶表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2142.
　SHEN Li-li,PENG Jia-he,LIU Hong,et al.Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(05):2142.
[2]娄桂予,周度金.法尼酯X受体的研究进展[J].陆军军医大学学报(原第三军医大学学报),2005,27(20):2088.
[3]王永超,刘红,彭家和,等.法尼酯X受体上调肝细胞中成纤维细胞生长因子21的表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):912.
　Wang Yongchao,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor upregulates fibroblast growth factor 21 expression in cultured liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(05):912.

更新日期/Last Update: 2010-03-05