[1]杜璐,张金海,阮怀珍.炎性痛大鼠脊髓和背根节P2X3受体的表达变化[J].第三军医大学学报,2010,32(02):99-102.
 Du Lu,Zhang Jinhai,Ruan Huaizhen.Expression change of P2X3 receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats[J].J Third Mil Med Univ,2010,32(02):99-102.
点击复制

炎性痛大鼠脊髓和背根节P2X3受体的表达变化(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第02期
页码:
99-102
栏目:
论著
出版日期:
2010-01-30

文章信息/Info

Title:
Expression change of P2X3 receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats
作者:
杜璐张金海阮怀珍
第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所
Author(s):
Du Lu Zhang Jinhai Ruan Huaizhen
Department of Neurobiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
关键词:
P2X3受体背根神经节脊髓角叉菜胶疼痛
Keywords:
P2X3 receptor dorsal root ganglion spinal cord carrageenan pain
分类号:
R322.81;R329.25;R441.1
文献标志码:
A
摘要:
目的  观察足底皮下注射角叉菜胶致炎后大鼠行为学及脊髓背角和背根神经节(dorsal root ganglion, DRG)P2X3受体表达的变化。  方法  通过热辐射刺激法及von Frey纤维丝测定大鼠痛阈,应用免疫组化技术观察足底皮下注射角叉菜胶后不同时间其相应节段(L4~L6)脊髓及背根神经节P2X3受体表达的变化。  结果  足底皮下注射角叉菜胶后动物出现热痛敏和机械性痛敏,热痛阈和机械痛阈均下降,于12 h达最低,72 h后恢复至正常水平。免疫组化显示相应节段(L4~L6)脊髓背角P2X3受体免疫反应在注射角叉菜胶24 h后明显增强(P<0.01),72 h表达强度最高,168 h恢复至正常,并且相应节段背根神经节P2X3受体阳性神经元数12 h开始明显增加(P<0.01),持续至72 h,168 h恢复至正常。  结论  角叉菜胶所致炎性痛大鼠,脊髓背角和背根神经节P2X3受体活化,表达明显上调,表明P2X3受体在炎性痛的发生、发展中起重要作用。
Abstract:
Objective  To investigate the expression change of P2X3 receptor in spinal dorsal horn and dorsal root ganglion (DRG) in rats with experimental inflammatory pain. Methods  Totally 30 SD rats received a subcutaneous  injection of carrageenan in the right paw to establish inflammatory pain model, and then divided randomly and equally into 5 groups, that is 6, 12, 24, 72 and 168 h after injection. Another 6 rats undergoing a subcutaneous injection of normal saline in the right paw served as control. Pain threshold was assayed by thermal radiation and von Frey filament in 2, 4, 6, 12, 24, 72 and 168 h after injection. Expression of P2X3 receptor in spinal dorsal horn and DRG of every group was measured by immunohistochemical staining (IHC). Results  After injection of carrageenan, thermal hyperalgesia and mechanical allodynia appeared in the rats. The thermal withdrawl latency (TWL) and 50% paw withdrawl threshold (PWT) were decreased obviously from 2 h, descended to the lowest at 12 h, then returned to the normal level after 72 h. The expression of P2X3 receptor in spinal dorsal horn was increased obviously from 24 h,while that at DRG was increased obviously from 12 h. Both of the expressions lasted for 72 h, and returned to the normal level at 168 h after injection. Conclusion  Thermal hyperalgesia and mechanical allodynia appear in the rats after carrageenan injection, and the P2X3 receptor is activated and up-regulated in the DRG and spinal cord, suggesting that P2X3 receptor may play an important role in the incidence and development of inflammatory pain.

参考文献/References:

杜璐,张金海,阮怀珍.炎性痛大鼠脊髓和背根节P2X3受体的表达变化[J]. 第三军医大学学报,2010,32(2):99-102.

相似文献/References:

[1]刘晓红,曾俊伟,张金海,等.神经病理性痛大鼠背根神经节P2X3受体表达及电生理学特性的改变[J].第三军医大学学报,2008,30(04):288.
 LIU Xiao-hong,ZENG Jun-wei,ZHANG Jin-hai,et al.Dorsal root ganglion P2X3 receptor expression and electrophysiological characteristics in rats with neuropathic pain[J].J Third Mil Med Univ,2008,30(02):288.
[2]方强,杨景,李为兵,等.P2X3受体在逼尿肌不稳定大鼠膀胱组织的表达及其意义[J].第三军医大学学报,2007,29(13):1355.
[3]周占松,宋波,黄华,等.前列腺炎疼痛对背根神经节细胞兴奋性的影响及P2X3表达的变化[J].第三军医大学学报,2006,28(11):1206.
[4]刘晓红,曾俊伟,肖智,等.地塞米松快速抑制大鼠背根神经节细胞中ATP所致的钙升高[J].第三军医大学学报,2009,31(14):1329.
 LIU Xiao-hong,ZENG Jun-wei,XIAO Zhi,et al.Dexamethasone rapidly inhibits ATP-induced intracellular calcium concentration increase in rat dorsal root ganglion neurons[J].J Third Mil Med Univ,2009,31(02):1329.
[5]梁涛,李淑蓉,邓其跃,等.NOV在嗅鞘细胞中的表达及活性检测[J].第三军医大学学报,2005,27(07):596.
[6]黄华,阮怀珍,蔡文琴,等.大鼠坐骨神经损伤后ATP对背根节神经元兴奋性的影响[J].第三军医大学学报,2004,26(03):0.[doi:10.16016/j.1000-5404.2004.03.009 ]
 HUANG Hua,RUAN Huai zhen,CAI Wen qin,et al.[J].J Third Mil Med Univ,2004,26(02):0.[doi:10.16016/j.1000-5404.2004.03.009 ]
[7]肖桃元,晏良遂,程天民,等.前列腺素对周围神经再生的有害影响及其合成抑制剂消炎痛的作用[J].第三军医大学学报,1989,11(06):0.[doi:10.16016/j.1000-5404.1989.06.011 ]
[8]郑文玲,陈艳荣,欧娟娟,等.奥沙利铂致大鼠慢性外周神经毒性的背根神经节内神经递质类物质靶标定量检测研究[J].第三军医大学学报,2020,42(07):684.
 ZHENG Wenling,CHEN Yanrong,OU Juanjuan,et al.Quantitative detection of targeted neurotransmitters in dorsal root ganglia in rats with chronic peripheral neurotoxicity caused by oxaliplatin[J].J Third Mil Med Univ,2020,42(02):684.

更新日期/Last Update: 2010-01-21